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A Silver Ion Mismatched Universal Interval Ultrafast Amplification Colorimetric Sensor

A colorimetric sensor and silver ion technology, applied in the field of heavy metal detection, can solve the problems of complex pretreatment, unsuitable for rapid detection, and long detection time.

Active Publication Date: 2021-08-20
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods have the advantages of high sensitivity, wide detection range, and are suitable for the analysis of various samples. However, these methods also have complex pretreatment, require large-scale instruments and professional personnel to operate, high maintenance costs, long detection time, and are not suitable for on-site rapid detection. and other shortcomings

Method used

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  • A Silver Ion Mismatched Universal Interval Ultrafast Amplification Colorimetric Sensor
  • A Silver Ion Mismatched Universal Interval Ultrafast Amplification Colorimetric Sensor
  • A Silver Ion Mismatched Universal Interval Ultrafast Amplification Colorimetric Sensor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Embodiment 1 qualitative experiment

[0062] In this embodiment, ultrapure water artificially added with different concentrations of silver ions is used as the sample to be tested to illustrate the use of the sensor of the present invention and the method of the present invention.

[0063] 1. Construction of sPCR device

[0064] The temperature change of the sPCR device is realized through a 95°C high-temperature water bath and a 58°C medium-temperature water bath. A Light Cycler capillary (20 uL, 04 929 292001, Roche) was used as the sPCR sample chamber. Through rapid centrifugation, the samples will be collected at one end of each capillary; after the centrifugation, the capillary with the sample is fixed on a special plastic support.

[0065] 2. sPCR reaction

[0066] The sPCR reaction system is shown in the table below:

[0067] Table 1

[0068] Reactive components Final concentration template 0.01μM Ex Taq DNA Polymerase 1.5U / mL ...

Embodiment 2

[0079] Embodiment 2 quantitative detection

[0080] In this embodiment, on the basis of the qualitative detection described in Embodiment 1, the quantitative detection of silver ions in the sample to be tested is realized by using different concentrations of silver ion solutions to prepare a standard curve.

[0081] With respect to embodiment 1, this embodiment has increased the step of preparing standard curve, specifically as follows:

[0082] Utilize the silver ion solution of known concentration, prepare the sPCR reaction system (in described reaction system, remove the silver ion final concentration and embodiment 1 difference of silver ion final concentration of 5nM, 30nM, 90nM, 120nM, 160nM, 200nM and 250nM respectively Except that all the other are the same as in Example 1), the sPCR product can form a G quadruplex under suitable conditions, and catalyze the ABTS color development, and the standard curve is as follows image 3 shown.

[0083] The regression equation ...

Embodiment 3

[0085] Embodiment 3 specificity experiment

[0086] This example is used to verify the specificity of the sensor and method of the present invention.

[0087] In this example, by adding 100nM Ag + , and 100µM Pb 2+ 、Cr 3+ , Zn 2+ 、Cd 2+ Join respectively in the reaction system, carry out specificity experiment according to the method described in embodiment 1, experimental result is as follows Figure 4 As shown, it is shown that the sensor and method of the present invention have higher specificity for silver ions.

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Abstract

The invention belongs to the field of heavy metal detection and specifically discloses a silver ion mismatch type universal partition ultrafast amplification colorimetric sensor. The present invention cleverly designs primers and templates (shown in SEQ ID NO.1‑3), so that templates can be amplified ultrafast in the presence of silver ions, and the amplified products can form G quadruplexes in a suitable environment . The peroxidase-like activity of the G quadruplex is further used for color development, which solves the difficult problem of visual detection of traditional PCR products and realizes the rapid and visual detection of silver ions. Moreover, the sensor and method provided by the present invention have the characteristics of high specificity and high sensitivity to silver ions, and the detection results are more objective and accurate.

Description

technical field [0001] The invention belongs to the field of heavy metal detection, and in particular relates to a silver ion mismatch type universal partition ultrafast amplification colorimetric sensor. Background technique [0002] Silver is a chemical element, chemical symbol Ag, atomic number 47, a silver-white transition metal. Silver exists in a small amount in free form in nature, mainly in silver-containing compound ores. Silver has stable chemical properties, low activity, high price, good thermal and electrical conductivity, is not easy to be corroded by chemicals, soft and malleable. Its reflective rate is extremely high, which can reach more than 99%. The most important compound of silver is silver nitrate. An aqueous solution of silver nitrate is often used as eye drops in medicine, because silver ions can strongly kill germs. At the same time, silver ions are also highly toxic, and are easily enriched by organisms in water, thereby inhibiting the activity ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/686
CPCC12Q1/686C12Q2521/337C12Q2525/186C12Q2565/607
Inventor 许文涛罗云波黄昆仑杜再慧田晶晶
Owner CHINA AGRI UNIV
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