The invention provides a method for accurately identifying
disease resistance of newly-planted sugarcane white leaf. The method comprises:
cutting sugarcane stalks infected with sugarcane white leaf
phytoplasma, performing juicing, adding 10 times amount of
sterile water to prepare an inoculating liquid;
cutting the sugarcane stalks which are a sugarcane material to be tested into stalk sections with buds, soaking the sections in running
tap water for 48h, performing hot
water treatment at 50 DEG C for 2h, and performing soaking with an insecticidal and sterilizing agent for 10min; performingcoating
inoculation with a
plastic film, and performing
moisture preservation at 25 DEG C for 24h; planting an inoculating material in a plastic
barrel, and placing the
barrel in a 20-30 DEG C insecticidal
greenhouse for culture; and investigating infected
plant rate, and performing
disease resistance evaluation according to 1-5 grade standards. The method of the invention firstly establishes a set of accurate and efficient method for identifying
disease resistance of newly-planted sugarcane white leaf, and provides
technical support for sugarcane white leaf resistance breeding. The
pathogen inoculating liquid is sprayed onto the sugarcane seed and the
coating inoculation with a
plastic film is performed, so that a disease
habitat condition is provided, and the result is objective, true and reliable. The
inoculation is quick and simple, the
operability is strong, and the work efficiency is high. After the inoculation, the sugarcane white leaf has obvious incidence, high sensitivity andgood reproducibility.