172 results about "Conjugated protein" patented technology

The invention encompasses micelle assemblies, compositions having micelle assemblies, and methods for preparing micelle assemblies and compositions thereof. The invention also encompasses a prolamine protein conjugated to a polymer, such as a polyethylene glycol (PEG) chain, which conjugates can be used to prepare micelle assemblies. The invention further encompasses methods of encapsulating molecules using the conjugates of the invention. The micelle assemblies can be used for a variety of applications, such as treating cancer, targeting tumors, reducing the toxicity of a drug in vivo, increasing the efficacy of an encapsulated agent in vivo, protecting an encapsulated agent against degradation, and enhancing the water solubility of a drug or other agent.

Novel site-specific mono-conjugates of Granulocyte Colony Stimulating Factor (G-CSF) are hereby described, with analogues and derivatives thereof, which stimulate proliferation and differentiation of progenitor cells to mature neutrophiles. These conjugates have been obtained using transglutaminase to covalently and site-specifically bind a hydrophilic, non-immunogenic polymer to a single glutamine residue of the human G-CSF native sequence and analogues thereof. These novel site-specific mono-conjugated derivatives are recommended for therapeutic use since they are stable in solution and exhibit significant biological activity in vitro and a longer bloodstream half-life, as compared to the non-conjugated protein, with a consequent prolonged pharmacological activity.

This invention provides Sso7-polymerase conjugates that exhibit improved activity in a polymerase reaction.

Methods and compositions for using N-hydroxysuccinimide and N-hydroxysulfosuccinimide to covalently couple protein(s) to the surface of red blood cells universally, rapidly and conveniently are provided. In one embodiment, the compositions promote immune tolerance in a subject. One embodiment provides autologous or allogenic red blood cells having whole protein(s) of interests conjugated to proteins on or within the plasma membrane of the red blood cells, wherein the conjugated proteins display at least one antigen to which immune tolerance is desired. The proteins are conjugated to the RBCs using carbodiimide chemistry. In a preferred embodiment, the whole proteins are conjugated using EDC in combination with NHS or sulfo-NHS.

A combination of “bottom up” and “top down” MS analysis of posttranslational modifications in complex proteins is described. The method comprises digestion of the protein with an enzyme that forms larger peptide fragments than trypsin (>3000 D), performing HPLC with the fragments and applying a new data acquisition strategy using on-line coupling with e.g. LTQ-FTMS, a hybrid mass spectrometer that couples a linear ion trap with a Fourier transform ion cyclotron resonance (FTICR) cell. The method is applied to analysis of posttranslational modifications of protein isoforms.

The present invention provides a novel antibody-RNase conjugate which is a single chain protein, providing both the specificity of its antibody portion and the RNase activity of its RNase portion, resulting in an antigen specific effectiveness against cells when applied in vivo or in vitro, wherein the RNase portion is effectively cytotoxic in at least a fraction of cells presenting the antigen, e.g. after internalization by endocytosis. In detail, the present invention provides scAb-RNase conjugate having the principal structure of scFvFc-RNase. This structure could also be shown to allow the effective production of antigen-specific and cytotoxic conjugate protein in cell culture, the conjugate having a high activity with respect to antigen specificity and cyto toxicity.

A protein utilizing an anti-gold antibody and a gold-binding side which is a part of the anti-gold antibody is constructed. This protein is capable of specifically binding to gold. This protein or a complex protein containing such a protein can be used for the detection of a target substance.

Disclosed herein are methods of protein quantification and normalization using haloalkylated tryptophan fluorescence. Complex protein samples, i.e., samples that each contain 1,000 or more distinct proteins, from diverse sources that do not have common protein profiles are treated with a halo-substituted organic compound (i.e. haloalkane) that reacts with tryptophan residues to form fluorescent products. Irradiation of the samples with ultraviolet light and the detection and quantification of the resultant fluorescent emissions from all proteins in each sample are then used to obtain comparative values for total protein content among the various samples. The values thus obtained are found to be valid indications of comparative total protein content, despite the fact that the tryptophan levels vary widely among the various proteins in any single sample and the samples, due to the diversity of their origins, tend to differ among themselves in the identities and relative amounts of the proteins that they contain. Protein samples are also normalized to correct for differences in sample dilution, sample loading, and protein transfer inconsistencies, by using stain-free detection of total protein in each of the samples, or detection of subsamples within each sample.

The present invention discloses one kind of retinol conjugated protein and its preparation process and specific antibody. The present invention expresses retinol conjugated protein effectively for the first time, and the expressed retinol conjugated protein has property near to that of natural retinol conjugated protein (RBP4). Immunizing animal with the expressed retinol conjugated protein can obtain antibody capable of recognizing natural retinol conjugated protein in human body sensitively. The present invention fills in a gap in RBP4 antiserum.

The invention provides a functional food set meal capable of losing weight scientifically and healthily. The functional food set meal comprises a conjugated protein meal replacement solid combination, a momordica grosvenori dietary fiber composite solid combination, a chia seed solid combination, a chitosan oligosaccharide solid combination, a fruit, vegetable and grain solid combination, a probiotics solid combination and a wheat seedling dietary fiber solid combination. Compared with the prior art, the functional food set meal is a nutritional food set meal which is beneficial to losing weight and is studied and developed based on the scientific weight losing theory and method, and according to the design and development theory of the set meal, on the premise of guaranteeing health, intake and consumption of energy are reasonably controlled, calorie supply and demand balance is set up, a biont has no redundant energy accumulation, meanwhile, internal fat is mobilized to be decomposed, energy is released to meet the need of the biont, and therefore the aim of losing weight is achieved. The functional food set meal has the advantages of adopting body health as the premise, balancing nutrition, reasonably controlling the energy and consumption, setting up energy negative balance, protecting the liver, adjusting the liver metabolism, mobilizing fat decomposition to release energy and the like.

The invention discloses compound protein powder for resisting age-related sarcopenia, and preparation methods thereof. The compound protein powder comprises, by weight, 60-80 parts of whey protein, 10-30 parts of collagen peptide, 1-5 parts of amino acids, 0.1-0.25 part of vitamin, 0.005-0.02 part of carotene, 5-20 parts of MCT and 0.01-0.05 part of creatine. The compound protein powder may also comprise 3-20 parts of lecithin. The preparation methods comprise a dry method and a wet method. The collagen peptide, the whey protein and the amino acids are mixed; MCT and vitamin are mixed; vitamin is mixed with carotene, and creatine; and then the above materials are mixed together. The compound protein powder can guarantee absorption of human body protein and promote growth of muscle, without aggravating age-related fat accumulation, and can play a good delay effect for age-related sarcopenia. The preparation process is stable; quality is controllable; and the compound protein powder can be produced in large scale.

The invention provides a test kit for measuring the content of retinol conjugated protein in serum of a human body and a preparation method of the test kit. The volume ratio of a reagent R1 and a reagent R2 is 3:1. The R1 comprises 0.01-4%w / w of emulsifiers, 0.1-2%w / w of sodium chloride, 0.05-2%w / w of sensitizers, 0.02%w / w of preservative and 30-100mmol / L of buffer solutions. The R2 comprises 0.1-0.5%w / w of latex particle combined with a retinol conjugated protein antibody, 0.1-1.0%w / w of bovine serum albumin, 0.02-2%w / w of Tween 20, 0.02-2%w / w of stabilizers 2, 0.02-2%w / w of protein protective agents, 0.02%w / w of preservative and 20-50mmol / L of buffer solutions. The test kit has wide linearity range, high sensitivity and stability and good clinical application prospect.

A pharmaceutical preparation comprising one of the botulinum neurotoxins from Clostridium botulinum of types A, B, C, D, E, F or G or a mixture of two or more of these neurotoxins, wherein the neurotoxin or the mixture of neurotoxins is free of the complexing proteins which naturally form the botulinum neurotoxin complexes together with the neurotoxins.

The present invention relates to highly conjugated proteins and methods for making such proteins. In particular, the present invention relates to methods for linking additional sites to a protein for conjugation with activated polyethylene glycol (PEG) linkers, without denaturing the protein. The invention also relates to highly conjugated proteins with decreased immunogenicity and increased circulating half-life.

A conjugate protein comprising an IL-2 and a soluble TGF-beta type II receptor type B and its use in cancer immunotherapy are described.

The invention discloses a method of detecting whether an interaction exists between nucleic acid protein and target protein based on biochip. The method comprises the following steps: (1)adding a plurality of groups of nucleic acid acquisition probes into a biological sample system containing the target protein to form a target-protein-nucleic-acid-conjugated-protein-nucleic-acid-acquisition-probe compound, and the nucleic acid acquisition probes containing at least one section of sequence capable of integrating with the nucleic acid conjugated protein; (2) separating the target-protein-nucleic-acid-conjugated-protein-nucleic-acid-acquisition-probe compound through molecules capable of specifically integrating with the target protein, and then recovering the nucleic acid acquisition probes; (3) carrying out hybridization of the nucleic acid acquisition probes recovered in the step 2 and a plurality of single chain immobilized probes which are fixed on the substrate of the biochip and are corresponding to the nucleic acid acquisition probes, and the nucleic acid sequence of the immobilized probes complementing the corresponding nucleic acid acquisition probes or one chain of the nucleic acid acquisition probes; (4) detecting the hybridization results to see whether the interaction exists between the nucleic acid conjugated protein and the target protein.

The invention aims to provide a bacillus licheniformis and application thereof. The preservation number of a bacterial strain of the bacillus (Bacillus lincheniformis) LV005 is CCTCC M 2013262. The bacillus licheniformis LV005 disclosed by the invention is screened from an alimentary canal of a prawn, and has the advantages of improving the composition and the number of dominant microbial populations in the alimentary canal of the prawn, forming and maintaining good microecological balance of the alimentary canal, and resisting the adhesion and the proliferation of viruses in the alimentary canal through nutrition competition, space competition, bacteriocin secretion and the like. The bacterial strain can activate the expression of prophenoloxidase, superoxide dismutase, lysozyme, heat shock protein, lipopolysaccharide-glucan conjugated protein, signal transduction, transcriptional activation factors and the like of the prawn with relevant pathogenic infection resistant molecules, and retard the proliferation rate of the viruses in the body of the prawn, so that the bacterial strain has various biological functions of improving the activity of the protease, the lipase and the amylase of the prawn, promoting the digestion capacity of the prawn and the digestion for feed nutrients, improving the stress response resistant capacity of the prawn and the like.

A pharmaceutical preparation comprising one of the botulinum neurotoxins from Clostridium botulinum of types A, B, C, D, E, F or G or a mixture of two or more of these neurotoxins, wherein the neurotoxin or the mixture of neurotoxins is free of the complexing proteins which naturally form the botulinum neurotoxin complexes together with the neurotoxins.

The invention relates to a nutritious compound protein powder, which eliminates the defect that the present food which is used to supplement protein can increase the risk of cardiovascular disease because of the increase of fat and cholesterol. The compound protein powder includes 8-12 shares (by weight) human collagen protein, 8-12 shares (by weight) bovine coloctrum, 250-350 shares (by weight) soybean protein and 550-650 shares (by weight) whey protein.

The invention discloses a preparation method of a composite protein foaming agent. The method comprises: preparing a mixed protein solution; performing microwave and supersonic wave cooperative treatment to the mixed protein solution at the pH value of 1.5-3.0 to obtain a micro-granulated composite protein solution; performing high-pressure microjet to the micro-granulated composite protein solution to obtain a homogeneous composite protein solution; and drying the homogeneous composite protein solution to obtain the protein foaming agent. Through a microwave and supersonic wave cooperative effect, heterogenous protein is induced to undergo aggregation and recombination, thereby forming specific composite protein aggregate particles. Further, the particle size of the composite protein aggregate particles is regulated with the assistance of high-pressure microjet. The adaptability to the environmental condition of the composite protein foaming agent is improved.

The invention relates to 5-Br-PADCAP even phase automatic analysis method and liquid stabilizer of serum copper, zinc, iron; it can be widely used in medicine and biochemistry technique filed, which is characterized in that: by serum copper, zinc, iron and quick decomposition of conjugated protein and application of hematocyanin stabilization technology with protein dissociation agent, the copper, zinc, iron in serum is cleavaged from the conjugated protein quickly on condition of even phase, and chromogenic reaction is happened with water soluble pyridylazo dye 5-Br-PADCAP under condition that surface activator, content measurement of copper, zinc, iron in sample can be realized on automatic analyzer in measurement mode of two-point end method. The liquid measurement agent with the method is stable, convenient, and capability of anti-interference is strong, without pollution to tube of automatic analyzer, it fits for batch automatic detection of copper, zinc, iron especially.

The invention discloses a retinol conjugated protein detection kit. The kit comprises a reagent R1 and a reagent R2, wherein the reagent R1 is a buffer solution, the reagent R2 is the mixture of retinol conjugated protein antibody sensitized polystyrene latex particles and the buffer solution. The retinol conjugated protein detection kit has the advantages that the kit is simple and quick to detect, high in sensitivity, good in accuracy, good in anti-interference capacity and low in production cost; a lipoprotein a (retinol conjugated protein) detection method adopted by the kit is a latex enhanced immuno-turbidimetry and enables the lipoprotein a (retinol conjugated protein) detection to be more economical, convenient and quicker, and the retinol conjugated protein detection kit is applicable to automatic biochemical analyzers of most of hospitals and especially can achieve quick quantitative detection for emergency treatment.

The present invention provides compositions and methods for assessing profiles of catalytically active enzymes in compositions containing a plurality of proteins. In preferred embodiments, the enzyme is a hydrolase, most preferably a cysteine protease. The methods described herein use activity based probes (“ABPs”) that have an affinity moiety for directing the binding of the ABP to one or more catalytically active target enzymes, a reactive group for forming a covalent bond at an active site of the target enzyme(s), and a TAG (e.g., a detectable label, preferably a fluorophore). One or more ABPs may be combined with a protein-containing sample under conditions for binding and reaction of the ABP(s) with target enzyme(s) that are present in the sample. The resulting products may then be used to assess the active enzyme profile of the sample, and can be correlated to the presence, amount, or activity of one or more target enzyme(s) present in the original complex protein mixture.

The invention aims to provide a bacillus pumilus bacterial strain of which the preservation number is CCTCC M2013263. The bacillus pumilus disclosed by the invention can improve the composition and the number of dominant microbial populations in an alimentary canal of a prawn to form and maintain good microecological balance of the alimentary canal, and withstand the adhesion and the proliferation of viruses in the alimentary canal through competition for nutrition, competition for space or secretion of bacteriocin and the like. The bacillus pumilus can activate the expression of prophenoloxidase, superoxide dismutase, lysozyme, heat shock protein, lipopolysaccharide-glucan conjugated protein, signal transduction and transcriptional activation factors and the like of the prawn with relevant pathogenic infection resistant molecules, and retard the proliferation rate of the viruses in the body of the prawn. The bacillus pumilus disclosed by the invention can be added to raw materials of a prawn feed, and can also be directly applied to the prawn breeding water environment.

The invention provides a colloidal gold immunocolorimetry kit for detecting retinol conjugated protein (RBP) and a preparation method of the kit and belongs to the field of in-vitro diagnostic reagents. The kit is used for judging whether the situation of individual acute kidney injury occurs or not. According to the method, colloidal gold particles are adopted as a carrier, a retinol conjugated protein (RBP for short) antibody and the colloidal gold particles are coupled, RBP protein in a sample makes contact with the coupled gold particles in a corresponding detection device, an antigen-antibody-gold compound is formed, the light absorbance of the gold particles under detection wavelength is changed, the change amount of the light absorbance is positively correlated with the concentration of RBP protein, and then the purpose of detecting RBP protein in the sample is achieved. The kit has high analysis sensitivity.