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32 results about "Prophenoloxidase" patented technology
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Prophenoloxidase (proPO) is a modified form of the complement response found in some invertebrates, including insects, crabs and worms. A major innate defense system in invertebrates is the melanization of pathogens and damaged tissues. This important process is controlled by the enzyme phenoloxidase (PO). The conversion of prophenoloxidase to the active form of the enzyme can be brought about by minuscule amounts of molecules such as lipopolysaccharide, peptidoglycan and beta-1,3-glucans from microorganisms.
Tobacco polyphenol oxidase gene NtPPO1 and site-directed mutagenesis method and application thereof
InactiveCN107653256AReduce expressionReduce PPO enzyme activityHydrolasesOxidoreductasesNicotiana tabacumPolyphenol oxidase
The invention discloses a tobacco polyphenol oxidase gene NtPPO1 and a site-directed mutagenesis method and application thereof. The nucleotide sequence of the tobacco polyphenol oxidase gene NtPPO1 is shown in SEQ ID:No.1, the coded amino acid sequence is shown in SEQ ID:No.2. The invention further discloses a clone method of the tobacco polyphenol oxidase gene NtPPO1. The clone method comprisesthe specific steps that firstly, cDNAs of tobacco leaves are synthesized; secondly, PCR amplification of the NtPPO1 gene is conducted; thirdly, a CRISPR / Cas9 carrier of the NtPPO1 gene is constructed;fourthly, sequencing detection of the NtPPO1 gene mutation is conducted. The NtPPO1 gene has a wide application prospect in preventing tobacco browning. The tobacco polyphenol oxidase gene and the encoded protein thereof provide the gene and technology support for crops especially for anti-browning breeding of tobaccos.
Owner:YUNNAN ACAD OF TOBACCO AGRI SCI
Bacillus licheniformis and application thereof
InactiveCN104388333AEnhance non-specific immune functionPromote digestionBacteriaAnimal feeding stuffBiotechnologyNutrition
The invention aims to provide a bacillus licheniformis and application thereof. The preservation number of a bacterial strain of the bacillus (Bacillus lincheniformis) LV005 is CCTCC M 2013262. The bacillus licheniformis LV005 disclosed by the invention is screened from an alimentary canal of a prawn, and has the advantages of improving the composition and the number of dominant microbial populations in the alimentary canal of the prawn, forming and maintaining good microecological balance of the alimentary canal, and resisting the adhesion and the proliferation of viruses in the alimentary canal through nutrition competition, space competition, bacteriocin secretion and the like. The bacterial strain can activate the expression of prophenoloxidase, superoxide dismutase, lysozyme, heat shock protein, lipopolysaccharide-glucan conjugated protein, signal transduction, transcriptional activation factors and the like of the prawn with relevant pathogenic infection resistant molecules, and retard the proliferation rate of the viruses in the body of the prawn, so that the bacterial strain has various biological functions of improving the activity of the protease, the lipase and the amylase of the prawn, promoting the digestion capacity of the prawn and the digestion for feed nutrients, improving the stress response resistant capacity of the prawn and the like.
Owner:YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
Insect, insect hemolymph and assembly active substance thereof and application
The invention relates to the field of biotechnology, and particularly relates to the bioactivity of the insect, insect hemolymph and assembly active substance thereof in activating a prophenoloxidase activation system or / and Toll signal transmission path system and an application of the insect, insect hemolymph and assembly active substance thereof in microorganism and related molecular mode detection thereof, preparation of the assembly active substance of prophenoloxidase activation system and separation and purification of antibacterial peptide. The invention also provides an insect hemolymph collection method and the storage method and conditions of the hemolymph; under the conditions, the stability of the insect hemolymph is better, and the functions of the insect hemolymph can be better played.
Owner:SHENYANG PHARMA UNIVERSITY
Bacillus pumilus bacterial strain application thereof
ActiveCN104388337ASimple compositionOptimize quantityBacteriaMicroorganism based processesConjugated proteinBacterial strain
The invention aims to provide a bacillus pumilus bacterial strain of which the preservation number is CCTCC M2013263. The bacillus pumilus disclosed by the invention can improve the composition and the number of dominant microbial populations in an alimentary canal of a prawn to form and maintain good microecological balance of the alimentary canal, and withstand the adhesion and the proliferation of viruses in the alimentary canal through competition for nutrition, competition for space or secretion of bacteriocin and the like. The bacillus pumilus can activate the expression of prophenoloxidase, superoxide dismutase, lysozyme, heat shock protein, lipopolysaccharide-glucan conjugated protein, signal transduction and transcriptional activation factors and the like of the prawn with relevant pathogenic infection resistant molecules, and retard the proliferation rate of the viruses in the body of the prawn. The bacillus pumilus disclosed by the invention can be added to raw materials of a prawn feed, and can also be directly applied to the prawn breeding water environment.
Owner:湖北宜水环保科技有限公司
Bacillus subtilis strain and application thereof
InactiveCN104312961AEnhance non-specific immune functionImprove anti-stress response abilityBacteriaAnimal feeding stuffConjugated proteinExcretion process
The invention aims to provide bacillus subtilis with a collection number of CCTCC M2013264. The bacillus subtilis preparation provided by the invention has a variety of biological functions of improving the composition and number of advantageous floras in alimentary canals of prawns, forming and maintaining good micro-ecological balance in the alimentary canals, resisting adherence and propagation of viruses in the alimentary canals by virtue of nutrient competition, spatial competition or bacteriocin excretion and the like, being able to enhance nonspecific immunity of the prawns to reduce the chance of virus infection, activating expressions of prophenoloxidase, hyperoxide dismutase, lysozyme, heat shock protein, lipopolysaccharide-glucosan conjugated protein, signal conduction and transcription activator and the like of the prawns and antipathogen infection related molecules to slow down the propagation rate of viruses in bodies of the prawns, improving activity of protease, lipase and amylase of the prawns, promoting digestive ability of the prawns and digestion of feed nutrients, improving stress reaction resistance of the prawns, and the like.
Owner:YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
Serpin-3 and functions, preparation method and application thereof
ActiveCN106478812AIncrease productionPeptide/protein ingredientsAntipyreticNucleotideAntimicrobial peptides
The invention relates to the technical field of bio-medicine and relates to a structure of serpin-3, an obtaining method and new physiological functions of the serpin-3 and application in the field of bio-medicine. The amino acid and nucleotide sequence of complete sequences of the serpin-3 is shown in SEQ ID NO:1. The amino acid and nucleotide sequence of mature peptide of the serpin-3 is shown in SEQ ID NO:2. The invention further provides an analogue or active fragments of the recombinant serpin-3. The natural and recombinant serpin-3 and the analogue and part of fragments of the serpin-3 have an inhibition effect on serine protease, antimicrobial peptide synthesis and a system activating prophenoloxidase in vitro and vivo. The obtaining method of the natural and recombinant serpin-3 and the part of fragments or analogue of the serpin-3 is conventional, simple and high in yield.
Owner:SHENYANG PHARMA UNIVERSITY
PtgC1qR gene of C1q receptor of blue crab as well as coding protein and application thereof
ActiveCN108558994AClear vigorEnhanced inhibitory effectAntibacterial agentsCell receptors/surface-antigens/surface-determinantsCDNA libraryAntibacterial activity
The invention belongs to the technical field of molecular biology and particularly relates to a PtgC1qR gene of a C1q receptor of a blue crab as well as coding protein and application thereof. The PtgC1qR gene of the C1q receptor of the blue crab is as shown in a base sequence in a sequence table SEQ ID NO. 1. A cDNA library and a RACE technology are utilized for amplifying the blue crab to obtainthe cDNA of the PtgC1qR gene, and recombinant PtgC1qR protein has obvious phenol oxidase inhibition activity, antibacterial activity and microbial binding activity. The invention lays a foundation for disease control and gene-assisted selective breeding of the blue crab and has potential application value in the aspects of development of aquatic drugs, production of feed additives and the like.
Owner:INST OF OCEANOLOGY - CHINESE ACAD OF SCI
DNA numerator of hairpin RNA for expressing inhibit wheat kernel polyphenol oxidase and uses thereof
InactiveCN101508990ADoes not affect vegetative growthDoes not affect stress resistanceMicroorganismsVector-based foreign material introductionTriticeaeNucleotide
The invention discloses a DNA molecule expressing a hairpin RNA which inhibits polyphenol oxidase, and application thereof. The DNA molecule expressing the hairpin RNA which inhibits the polyphenol oxidase consists of an A fragment, a B fragment and a C fragment which are connected in sequence, wherein a nucleotide sequence of the A fragment is sequence 1 in a sequence table; the C fragment and the A fragment are reversely compensated; and a nucleotide sequence of the B fragment is sequence 2 in the sequence table. The DNA expressing the hairpin RNA which inhibits the polyphenol oxidase is started by a strong promoter of HMW glutelin subunit 1D*5 expressed by specific of wheat grain, expression of ppo gene of grain filling in transgenic wheat is obviously inhibited, PPO isoenzyme activity is reduced, and whiteness of wheat flour can be improved on the premise that vegetative growth and resistance of wheat are not affected.
Owner:CHINA AGRI UNIV
Crabs immune reinforcing agent high flux screening method
InactiveCN101643773AObvious immune boosting effectImprove throughputMaterial analysis by observing effect on chemical indicatorMicrobiological testing/measurementHigh fluxScreening method
The invention relates to an extracorporeal fast screening method of the crabs immune reinforcing agent, in particular to a crabs immune reinforcing agent high flux screening method, comprising the following steps: mixing the immune reinforcing agent to be tested and crabs phenol oxidase original crude extract; activating the activity of the prophenoloxidase in the crabs original crude extract; converting the prophenoloxidase into active phenol oxidase; changing the color of the phenol oxidase catalyzing substrate L-dihydroxyphenylalanine from achromatic color into a color product-chinone; according to the coloration degree of the chinone, computing the activity enhancement rate of the immune reinforcing agent to be tested to the phenol oxidase to be taken as evaluation basis of the extracorporeal screening crabs immune reinforcing agent. By testing the activity of the phenol oxides with a microplate reader, the method automates the activity test of the phenol oxidase, greatly improvesthe screening efficiency of the crabs immune reinforcing agent, and realizes the high flux screening of the immune reinforcing agent. The method can provide fast and effective route for the extracorporeal screening of the crabs immune reinforcing agent, and is applicable to massive screening and prescreening of the activity of the crabs immune reinforcing agent.
Owner:ZHEJIANG INST OF FRESH WATER FISHERIES
Prawn QM protein for regulating phenol oxidizing enzymes (PO) activity by interactively acting with hemocyanin
Since it was proposed to be a tumor suppressor, QM protein has attracted intensive studies in plants, animals and fungi. So far, however, the function of the QM protein remains unknown. In this investigation, it is found that the shrimp QM gene (designated as PjQM) is significantly up-regulated in virus-resistant shrimp, thereby suggesting that the PjQM is involved in shrimp immunity. The GST pull-down assays show that the PjQM protein interactes with shrimp hemocyanin and myosin, thus indicating that the PjQM protein might participate in prophenoloxidation (proPO) activation system of shrimp immunity. As revealed by the RNAi assays, it is demonstrated for the first time that the QM protein could regulate the activity of phenol oxidase (PO) through interaction with hemocyanin, a key enzyme in the proPO activation system of invertebrate antiviral immunity.
Owner:THIRD INST OF OCEANOGRAPHY STATE OCEANIC ADMINISTATION
Plutella xylostella serine protease inhibitor Serpin7 gene and application thereof
ActiveCN112094848AInhibit phenoloxidase PO activityIncreased phenoloxidase PO activityBiocideProtease inhibitorsAntiendomysial antibodiesNucleotide
The invention discloses a plutella xylostella serine protease inhibitor Serpin7 gene and an application thereof. A nucleotide sequence of the plutella xylostella serine protease inhibitor Serpin7 geneis shown as SEQ ID NO:1, and an amino acid sequence of the plutella xylostella serine protease inhibitor Serpin7 is shown as SEQ ID NO:2. After the recombinant protein Serpin7 is added for eating, the activity of plutella xylostella phenol oxidase PO can be strongly inhibited; after a polyclonal antibody anti-Serpin7 of Serpin7 is added for eating, the activity of phenol oxidase PO can be remarkably improved; and after RNAi silences the expression of the Serpin7 gene, the activity of plutella xylostella phenol oxidase PO is improved, and plutella xylostella larvae generate the strong melanization reaction, so that most plutella xylostella cannot normally pupate, and those which can pupate cannot successfully emerge. The result shows that the plutella xylostella Serpin7 is a negative regulatory factor of innate immunity in insects, can be used as a novel molecular target for biological prevention and treatment, is used for preventing and controlling cruciferous vegetable pests, and hasgood biological prevention and treatment potential and application prospect.
Owner:SOUTH CHINA AGRI UNIV
Hevea brasiliensis polyphenol oxidase, its coding gene and application
The invention discloses a hevea brasiliensis polyphenol oxidase. The gene is intronless, has a cDNA sequence with a length of about 1869 bp, a sequence of SEQ ID NO: 1 shown in a sequence table, and is named HbPPO2. The HbPPO2 codes 592 amino acids, has a sequence of SEQ ID NO: 2 shown in the sequence table, and can be acidic protein a molecular weight of 67 kDa, an isoelectric point of 6.66, and an electrical charge of -0.4. The enzyme at least exists in buds, leaves, seeds, calluses and suspension cells, and the protein level is induced at least by pathogen infection and mechanical damage. The enzyme or its derivative protein at least can be used in food and industrial fields to bleach or oxidize colored substances. Utilizing the antisense expression of part or all of the sequences of the gene can at least inhibit the browning phenomenon in hevea brasiliensis tissue culture. The functional derivative gene over-expression obtained through the gene at least can improve the disease and pest resistance of hevea brasiliensis.
Owner:RUBBER RES INST CHINESE ACADEMY OF TROPICAL AGRI SCI
Method for regulating aquaculture water quality in shrimp and shellfish polyculture mode
ActiveCN110759476AIncrease release resistanceImprove hydrophilicityWater contaminantsWaste water treatment from animal husbandryPolycultureFatty acid
The invention provides a method for regulating aquaculture water quality in a shrimp and shellfish polyculture mode, and belongs to the technical field of aquaculture. The method comprises the following steps: establishing a bacteria-algae synergistic system capable of regulating water quality, and providing microencapsulated aquaculture baits capable of slowing down deterioration of water quality, wherein the bacteria-algae synergistic system comprises an algae system attached to a porous carrier and a biofloc system with an added carbon source skeleton, the microencapsulated culture baits include a porous pellet core to which lipids are attached under vacuum, and a biodegradable polymer coating film covering the pellet core, and the thickness of the coating film is 1 nanometer or more and 1 micron or less. According to the method provided by the invention, a zero-water exchange effect is achieved by combining the synergistic effect of baits and the bacteria-algae system, and the hydrophobicity of the baits can be improved, so that the baits are not disintegrated, the carbon source cost of the biofloc system is reduced, the volume concentration of bioflocs and the concentration oftotal suspended solids are improved, and the content of Omega-3 fatty acid in an organism and the component content and activity of immune molecules such as phenol oxidase and hemolysin are improved.
Owner:ZHOUSHAN OCEAN UNIV SCI & TECH RES INST CO LTD
Materials and methods for diagnosis of peri-implant bone and joint infections using prophenoloxidase pathway
InactiveUS20170045532A1Sensitive highThe process is convenient and fastMicrobiological testing/measurementDisease diagnosisPeri implant boneJoint infections
Diagnostic assays and related systems, test samples and methods are described for detection of infection following implantation of a prosthetic material or device on and / or into bones and joints. A combination of at least two biomarkers is used, one specific to the pathogenic microbe and the other specific to the destruction of peri-implant tissues. The biomarkers are detectable in the peripheral blood, urine, fluid or tissue samples drawn from a peri-implant environment. They can also be detected with a blood or urine test that is highly sensitive for detecting specific structural microbial molecules and peri-implant tissue components.
Owner:PARVIZI JAVAD +1
Preparation method and application of soluble housefly MdproPO1 recombination protein
The invention relates to a preparation method and application of soluble housefly MdproPO1 recombination protein. The preparation method includes the steps of firstly, building a mdproPO1 / pET-30a recombination expression vector; screening the expression strain mdproPO1 / pET-30a / Rosetta of the high-expression MdproPO1 recombination protein; thirdly, performing fermental cultivation and induced expression of the expression strain; fourthly, performing denaturation and renaturation of MdproPO1 inclusion bodies. The preparation method has the advantages that housefly prophenoloxidase (MdproPO1) produced by an Escherichia coli expression system is high in expression quantity, a large amount of MdproPO1 inclusion bodies can be obtained through prokaryote recycling and crushing, and the soluble MdproPO1 recombination protein with enzymatic activity can be obtained through the denaturation and renaturation of the MdproPO1 inclusion bodies; the MdproPO1 recombination protein can be developed into fly spray, an oxidizing agent, a blackening agent and an immunizing agent and is applicable to multiple fields such as sanitation, healthcare, biology and chemical engineering.
Owner:UNIV OF JINAN
Composite micro-ecological preparation and application thereof
PendingCN111066951AImprove immunityIncrease vitalityLactobacillusAnimal feeding stuffBiotechnologyDismutase
The invention provides a composite micro-ecological preparation. The composite micro-ecological preparation is composed of a lactobacillus johnsonii microcapsule, ginseng extract and Chinese angelicaextract. The invention also provides an application of the above composite micro-ecological preparation in preparation of feed for improving an immune level of farmed animals. The composite micro-ecological preparation provided by the invention can improve the immunity level of the farmed animals, and improve the activity of alkaline phosphatase (AKP enzyme), superoxide dismutase (SOD enzyme) andphenol oxidase (PO enzyme) in serum and hepatopancreas of the farmed animals, thereby having great significance for improving the breeding level of the farmed animals in China.
Owner:福建御研农业科技有限公司
A kind of preparation method and application of soluble housefly mdpropo1 recombinant protein
The invention relates to a preparation method and application of soluble housefly MdproPO1 recombination protein. The preparation method includes the steps of firstly, building a mdproPO1 / pET-30a recombination expression vector; screening the expression strain mdproPO1 / pET-30a / Rosetta of the high-expression MdproPO1 recombination protein; thirdly, performing fermental cultivation and induced expression of the expression strain; fourthly, performing denaturation and renaturation of MdproPO1 inclusion bodies. The preparation method has the advantages that housefly prophenoloxidase (MdproPO1) produced by an Escherichia coli expression system is high in expression quantity, a large amount of MdproPO1 inclusion bodies can be obtained through prokaryote recycling and crushing, and the soluble MdproPO1 recombination protein with enzymatic activity can be obtained through the denaturation and renaturation of the MdproPO1 inclusion bodies; the MdproPO1 recombination protein can be developed into fly spray, an oxidizing agent, a blackening agent and an immunizing agent and is applicable to multiple fields such as sanitation, healthcare, biology and chemical engineering.
Owner:UNIV OF JINAN
Pteromalus puparum venom Kazal serine proteinase inhibitor polypeptide and its application
The invention discloses a Pteromalus puparum venom Kazal serine proteinase inhibitor polypeptide which has an amino acid sequence represented by SEQ ID NO:2. The invention simultaneously discloses gene coding the Pteromalus puparum venom Kazal serine proteinase inhibitor polypeptide, wherein the gene has a nucleotide sequence represented by a 145-297 nucleotide sequence in SEQ ID NO:1, or has a nucleotide sequence having at least 70% homology with the 145-297 nucleotide sequence in the SEQ ID NO:1, or has a nucleotide sequence which can be hybridized with the 145-297 nucleotide sequence in the SEQ ID NO:1 at 40-55DEG C. The polypeptide of the invention is used for preparing Pteromalus puparum venom Kazal serine proteinase inhibitors, and the inhibitors can be used for inhibiting the activation of Pieris rapae or Papilio xuthus hemolymph PPO (prophenoloxidase).
Owner:ZHEJIANG UNIV
Method for regulating water quality in shrimp and shellfish polyculture
ActiveCN110759476BAvoid wastingIncrease contentWater contaminantsWaste water treatment from animal husbandryFatty acidPorous particle
Owner:ZHOUSHAN OCEAN UNIV SCI & TECH RES INST CO LTD
A high-temperature polyphenol oxidase and its application in the treatment of phenolic wastewater
ActiveCN113462701BHigh catalytic efficiencyImmobilised enzymesBacteriaOxidative enzymePolyphenol oxidase
Owner:玉凰生态科技股份有限公司
A technical method for identifying whether Bactrocera dorsalis is irradiated or not
InactiveCN104521926BRapid identificationSimple methodMicrobiological testing/measurementInsect catchers and killersBactrocera dorsalisAcetylcholine esterase
The invention discloses a method for irradiating oriental fruit flies. The method comprises the step that 60 Co gamma rays of dosage ranging from 40 Gy to 160 Gy are used for irradiating oriental fruit eggs, or first instar larvae and second instar larvae; or 60 Co gamma rays of dosage larger than 160 Gy are used for irradiating third instar larvae of oriental fruit flies. The invention further discloses a method for identifying whether oriental fruit flies are processed by irradiation reaching the standard. Live oriental fruit flies on detected plants or fruits are collected to detect the enzymatic activities of phenol oxidase, superoxide dismutase or / and acetylcholine esterase; if the enzymatic activity of the phenol oxidase is smaller than 80% of that of a contrast, the enzymatic activity of the superoxide dismutase is smaller than 85% of that of a contrast, or / and the enzymatic activity of the acetylcholine esterase is smaller than that of a contrast, it is determined that larvae are irradiated. By measuring the enzymatic activities of the phenol oxidase, superoxide dismutase (SOD) or / and acetylcholine esterase (AChE), whether oriental fruit flies are irradiated or not can be determined, and the larval period of the irradiated oriental fruit flies can be determined. The method is quite sensitive and reliable, and only one oriental fruit fly is needed during identification on the basis of the characteristic that the imported fruit intercept insect quantity is small.
Owner:INSPECTION & QUARANTINE TECH CENT OF GUANGDONG ENTRY EXIT INSPECTION & QUARANTINE BUREAU
A nematode preparation for controlling flower slugs and its preparation method and application
The invention discloses a nematode preparation for preventing and controlling flower slugs, a preparation method and application thereof, and belongs to the technical field of biological pesticides. The nematode preparation of the present invention includes preparation A and preparation B containing different active ingredients, the active ingredient of preparation A includes L-dopa, a substrate inhibitor of parasitic rod nematode and polyphenol oxidase; the active ingredient of preparation B includes polyphenol oxidase Quercetin, a structural inhibitor of phenoloxidase. When applying the nematode preparation of the present invention to prevent and control flower slugs, preparation A is applied to the rhizosphere soil surface of flowers in the evening; preparation B is sprayed on the buds and leaves of flowers in the afternoon of the same day. In the present invention, for the first time, the slug bio-control factor parasitic rod nematode and the two functional inhibitors of slug polyphenol oxidase are used in combination, and the field quick-acting performance of the parasitic rod nematode is greatly improved by using the slug feeding attraction, and through the preparation A and formulation B selected different application locations, application times and methods, which accelerated nematode infestation and lethal slugs.
Owner:INST OF PLANT PROTECTION SICHUAN ACAD OF AGRI SCI
Kit for specifically detecting peptidoglycan
InactiveCN105779567AGuaranteed to be free from interferenceHigh sensitivityMicrobiological testing/measurementHuman bodyInterference factor
The invention discloses a kit for specifically detecting peptidoglycan.The kit comprises prophenoloxidase, a chromogenic solution and peptidoglycan standards; in order to avoid influences of other interference factors such as glucan, the kit further comprises an alkali metal hydroxide treating agent or a biological buffer treating agent and a neutralization solution treating agent composed of alkali metal hydroxides and biological buffers; in order to store reaction results for a long time, the kit further comprises an acidic stopping solution.The kit can specifically detect the peptidoglycan in sterile body fluid of a human body, and interference of the interference factors such as the glucan is avoided; the sensitivity and specificity are both high, the coincidence rate of an identification result with blood culture strains is high, and clinical test demands can be met; detection can be conducted through an ordinary microplate reader in a laboratory, and the detection cost is reduced; after system reacting is stopped, the test results can be stored for a long time, repeated measurement can be conducted on the test results by means of the microplate reader again and again; operation is easy, long-time experience accumulation is not needed, and popularization is easy.
Owner:AUTOBIO DIAGNOSTICS CO LTD
Application of a cell wall skeleton as a feed additive for Penaeus vannamei
ActiveCN108094683BImprove immunityImprove the survival rate of breedingBacteriaAnimal feeding stuffFreeze-dryingCell Wall Skeleton
The invention relates to an application of a cell wall skeleton as a penaeus vannamei feed additive. According to the application, 0.8-1.2g of the cell wall skeleton is added to each kilogram of a penaeus vannamei basal feed, wherein the cell wall skeleton is obtained in the following manner of enabling separated and purified nocardiopsis lucentensis to be subjected to enlarged culture, performingultrasonic wave crushing on fermentation liquor, performing centrifuging, performing enzymolysis with trypsin, performing defatting, and performing freeze drying so as to obtain a white cell wall skeleton. According to the application disclosed by the invention, the activity of superoxide dismutase, nitricoxide synthase, phenoloxidase, lysozyme and glutathione peroxidase in hemolymph and hepatopancreas of penaeus vannamei can be notably increased, the concentration of malondialdehyde can be notably reduced, the respiratory burst activity of the hemolymph of the penaeus vannamei can be notablystrengthened, the immunity protection ratio of the penaeus vannamei can be obviously increased, and the breeding survival rate of the penaeus vannamei can be obviously increased.
Owner:TIANJIN UNIV OF SCI & TECH
A kind of serine protease inhibitor serpin7 gene of diamondback moth and its application
ActiveCN112094848BInhibit phenoloxidase PO activityIncreased phenoloxidase PO activityBiocideProtease inhibitorsBrassicaceaeCabbage moth
The invention discloses a Serpin7 gene of a diamondback moth serine protease inhibitor and an application thereof. The nucleotide sequence of the Serpin7 gene of the diamondback moth serine protease inhibitor is shown in SEQ ID NO: 1, and the amino acid sequence thereof is shown in SEQ ID NO: 2 shown. Feeding the recombinant protein Serpin7 can strongly inhibit the PO activity of the diamondback moth phenol oxidase; feeding the Serpin7 polyclonal antibody anti-Serpin7 can significantly increase the phenol oxidase PO activity; after RNAi silences the Serpin7 gene expression, the diamondback moth phenol oxidase PO activity increases High, the larvae of the diamondback moth produced a strong blackening reaction, which caused most of the diamondback moths to fail to pupate normally, and those who could pupate could not successfully emerge. It is indicated that Serpin7 of diamondback moth is a negative regulator of innate immunity in insects, and can be used as a molecular target for new biological control for the control of cruciferous vegetable pests, and has good biological control potential and application prospects.
Owner:SOUTH CHINA AGRI UNIV
C1q receptor ptgc1qr gene of Portunus trituberculatus and its encoded protein and application
ActiveCN108558994BClear vigorEnhanced inhibitory effectAntibacterial agentsCell receptors/surface-antigens/surface-determinantsBiotechnologyCDNA library
The invention belongs to the technical field of molecular biology, and specifically relates to a C1q receptor PtgC1qR gene of Portunus trituberculatus and its encoded protein and application. The C1q receptor PtgC1qR gene of Portunus trituberculatus is shown in the base sequence of SEQ ID No.1 in the sequence table. The cDNA of PtgC1qR gene was amplified from Portunus trituberculatus by cDNA library and RACE technology, and the recombinant PtgC1qR protein had significant phenoloxidase inhibitory activity, antibacterial activity and microbial binding activity. The invention lays a foundation for disease prevention and control of portunus trituberculatus and gene-assisted selective breeding, and has potential application value in the development of aquatic medicines and the production of feed additives.
Owner:INST OF OCEANOLOGY - CHINESE ACAD OF SCI
Penaeus orientalis feed capable of enhancing immunity, and preparation method and applications thereof
PendingCN111317076AImprove immunityNot easy to moldAnimal feeding stuffAccessory food factorsBiotechnologyVibrio parahaemolyticus
The invention discloses penaeus orientalis feed capable of enhancing immunity, and a preparation method and applications thereof. The feed consists of potassium chloride, zinc glycinate, aquatic plants, tubificidae, lactic acid bacteria, selenium-enriched yeast, a feed inducing agent, vitamins, a lindley eupatorium herb extract, a herba violae extract, mannan oligosaccharide, fatty acid, a commonandrographis herb extract, folic acid, an acanthopanax root extract, phenoloxidase PO, a devil's rush herb extract and glucoamylase. The feed is not easy to mould and go bad, has no caking and peculiar smell, no residues and no side effects, has good stability in water, and cannot be dispersed for 3-5 h; the outer membranes of vibrio cells can be destroyed; the pH of the intestinal tracts of penaeus orientalis is 5-7, and vibrio parahemolyticus can be killed, so that the survival rate of the penaeus orientalis can be enhanced by 15-25%, and body lengths can be increased by 30-50%; reproductivecapacity can be enhanced, and digestion rates can be increased; fast growth of the penaeus orientalis can be achieved, the lengths of the penaeus orientalis can reach 11-12 cm, and the weights of thepenaeus orientalis can be 10.5-12.5 g; and therefore, the feed is easy to industrialize and has wide application prospects.
Owner:YANCHENG INST OF TECH
Specific probe for detecting expression of prophenoloxidase gene of Eriocheir sinensis and application of specific probe
PendingCN114410805AReliable test resultsSimple and fast operationMicrobiological testing/measurementDNA/RNA fragmentationHigh fluxEriocheir japonica sinensis
The invention discloses a specific probe for detecting Eriocheir sinensis phenol oxidase progene expression and application thereof. According to the method, a probe sequence is designed in a conserved region of a gene, a flow cytometry is introduced to carry out high-throughput and high-precision automatic measurement by utilizing a principle that a fluorescently-labeled probe is specifically combined with mRNA of a target gene, a single cell sample point can be accurately identified, and intracellular fluorescence intensity can be quantitatively analyzed, so that quantitative information of the mRNA expression level of the target gene is obtained. The accuracy, reliability and repeatability of results based on tens of thousands of sampling points are far better than those of conventional methods such as manual observation counting. The method does not need an antibody, is low in cost, is high in flow-based high-flux automatic analysis efficiency, is good in reliability, and has great application potential.
Owner:TIANJIN NORMAL UNIVERSITY
A compound microbial preparation and its application in post-spraying of aquatic feed
ActiveCN107279469BGood water solubilityLittle loss of activityAnimal feeding stuffBiotechnologyBacillus licheniformis
The invention relates to the technical field of feed additives, in particular to a compound microbial preparation and its application in post-spraying of aquatic feed. The compound microbial preparation includes Enterococcus faecalis, Clostridium butyricum, Bacillus licheniformis and a compound protective agent, which are added to aquatic feed through a post-spraying process, which can effectively reduce the activity loss of microorganisms in the process of feed processing and feeding, thereby making the The survival rate and weight gain rate of turbot were significantly improved, and the weight gain rate increased by 19.8%, while the feed factor decreased by 14.3%. The activities of phenoloxidase and lysozyme in the blood of turbot in the experimental group increased respectively. 76.5% and 64.4%, which is beneficial to reduce the cost of feed, improve the immunity of aquatic animals, increase the breeding benefits, and the effect is remarkable.
Owner:青岛玛斯特生物技术有限公司 +1
Method for preparing aflatoxin B1 degradation preparation by mixed fermentation
ActiveCN110172482ADetoxificationEfficient detoxificationMicroorganismsMicroorganism based processesPectinaseAdjuvant
The invention discloses a technology of producing an aflatoxin B1 (AFB1) degradation preparation by mixed fermentation and application. Microorganisms used by the AFB1 degradation preparation preparedby the invention comprise aspergillus oryzae CGMCC 3.800, extracellular enzyme secreted by saccharomyces cerevisiae ATCC 204508, an amboceptor, an adjuvant and the like. The extracellular enzyme of the preparation comprises working enzyme (glucose oxidase, polyphenol oxidase) capable of degrading AFB1, assisting enzyme (pectinase, xylanase, cellulase) for improving degradation efficiency, and also comprises micromolecule amboceptor for transferring electron, adjuvant and the like. The working enzyme is oxidase, a catalytic process involves electron transfer and free radical generation, the micromolecule amboceptor and adjuvant can carry the electron into the interior of tissue of grain and oil, feed and related products, so as to degrade the AFB1 infected into the interior of tissue, namely multi-path and multi-step catalysis, thus realizing thorough detoxification of the AFB1. The preparation is convenient to use, small in dosage, high in biological security coefficient, and has gooddegradating effect on the AFB1 in mildewed grain and oil, feed and related products.
Owner:合肥停弦渡生物科技有限公司
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