33 results about "Respiratory burst" patented technology

The invention relates to a prenylated flavonoid, and applications thereof in preparing drugs used for treating inflammatory diseases. The prenylated flavonoid is a novel compound separated from artocarpus heterophyllus of Artocarpus, Moraceae, and is named as artoheteroid C. The structure of artoheteroid C is disclosed in the invention, possesses high inhibition activity on mouse neutrophil respiratory burst, can be used for preparing drugs used for treating inflammatory diseases, and can be used for clinical treatment of oxidative damages, such as rheumatoid arthritis, compensatory anti-inflammatory response syndrome, and systemic inflammatory response syndrome, caused by neutrophil excessive activation; and the IC50 value is 7.5<mu>M.

Provided is technology of external model construction of pseudosciaena crocea head kidney macrophage. The fish head kidney macrophage is horizontally and centrifugally separated by utilizing Percoll with 31% / 45% gradient and is cultured in an L15 compound culture medium under the condition that the temperature is 22 DEG C. Each liter of the culture medium contains 50ml fetal calf serum, 0.1 million UI penicillin / streptomycin, 0.05 million UI heparin, 10mmol 14-ethoxyl-piperazine ethanesulfonic acid (HEPES) and 0.5g glucose. The pH of the culture medium is 7.4. The method and the application have the advantages that (1) the proportion of the pseudosciaena crocea head kidney macrophage which is centrifugally separated by utilizing Percoll with 31% / 45% gradient in the separation cell is as high as 42%; (2) the purity of the adherence pseudosciaena crocea head kidney macrophage after liquid culture is as high as 90%; (3) the livability of the pseudosciaena crocea head kidney macrophage after one week culture is higher than 58%; and (4) a function checking experiment proves that LPS can remarkably affect the swallow function and breath eruption activity of the cultured pseudosciaena crocea head kidney macrophage.

The invention discloses a cigarette filter tip, comprising metallothionein. The cigarette filter tip has the advantages of eliminating the gas-phase free radicals in the cigarette flue gas, guarding against damage of cell ingredient and occurring and developing of diseases caused by the gas-phase free radicals in the cigarette flue gas, reducing the aggregation and activation of macrophage in the lung, and guarding against severe damage caused by respiratory burst and releasing active oxygen free radicals.

The invention relates to a preparation method and application of a prawn non-specific immunity reinforcing agent oligopeptide. The preparation method comprises the following steps of: mincing pacific alaskapollack dogmeat by a meat mincer and then placing in an enzyme reactor to heat and sterilize; triturating and then heating to 90 DEG C to preserve the temperature; sterilizing for 20 minutes in a constant temperature water bath; adding water and protease preparations to mix; carrying out enzymolysis under the condition of optimized enzymolysis process parameters; then heating enzymolysis liquid so that protease is inactivated; roughly filtering fishbones and centrifugalizing the enzymolysis liquid to obtain a soluble protein oligopeptide; ultrafiltering, purifying, freezing and drying and then obtaining protein oligopeptide powder. The protease preparations comprise Alcalase and Flavourzyme, and the enzymolysis process parameters comprise the enzymolysis temperature of 55 DEG C, the initial pH value of 8.5, the material and water ratio of 1:1 and the enzymolysis time of 3 hours. A macromolecule substance is intercepted by using a method for combining 8,000rpm high-speed centrifugalization and 100kDa ultrafiltration membrane ultrafiltrating purification, and 30 percent of protein oligopeptide with the molecular weight of smaller than 100Da is obtained. The oligopeptide powder replaces 5 percent of fish meal in prawn feed and can obviously improve the blood corpuscle density, the phenoloxidase activity, the respiratory burst activity, the SOD activity, the NOS activity and the specific growing rate of a prawn.

The present invention relates to a method of treating an inflammatory disorder in a subject with an effective amount of compound having the general formula (I) as described in the present application, under conditions effective to treat the inflammatory disorder. The present invention also relates to a method of inhibiting respiratory burst in neutrophils without or inhibiting degranulation in or bacterial killing by the neutrophils by contacting neutrophils with the compounds described above.

The invention provides a method for fish mononuclear / phagocyte separation, cultivation and functional verification. Fish phagocytic cells are successfully obtained through optimizing cultivation parameters and conditions; functions of the fish phagocytic cells are obtained by utilizing the phagocytic cells for phagocytizing exogenous chicken erythrocyte and detecting in-vitro respiratory burst. The method provided by the invention realizes in-vitro separation, conversion, continuous cultivation and the like of the fishphagocytic cells, requires few samples, is short in operation time, simple in flow and high in accuracy, and can be widely applied in the research of fish immune-related functions on a cellular level.

The invention provides a vitamin premix for nibea albiflora richardson juvenile fish, and a preparation method of the vitamin premix. The vitamin premix which is required by the nibea albiflora richardson juvenile fish during growth is developed according to the characteristics of nibea albiflora richardson, and is matched with the feed for use. Compared with the prior art, practical culture experiment using the feed which is prepared according to a formula of the disclosed vitamin premix for larimichthys crocea, and the vitamin premix for nibea albiflora richardson juvenile fish, which is provided by the invention, prove that the vitamin premix provided by the invention has the beneficial effects that the growth performance and the survival rate of the nibea albiflora richardson, and the feed utilization capacity can be significantly improved, and the blood and respiratory burst capability of the nibea albiflora richardson is improved. According to practical measurement results, the survival rate of the nibea albiflora richardson juvenile fish is as high as 100%; the weight gain rate is as high as 315%; the feed coefficient is 1.54; and the blood NBT vitality is 0.39.

The invention provides a Chinese herbal medicine immunopotentiator for groupers. The Chinese herbal medicine immunopotentiator comprises the following components in parts by weight: 20-30 parts of spatholobus stem, 15-25 parts of herba ecliptae, 10-20 parts of cortex phellodendri, 3-13 parts of coptis chinensis, 3-13 parts of fructus rubi, 3-13 parts of atractylodes macrocephala, 3-13 parts of peppermint and 3-13 parts of hemerocallis citrina baroni. The Chinese herbal medicine immunopotentiator is prepared from various green Chinese herbal medicines, small in toxic / side effect, unlikely to induct pathogenic bacteria resistance to drugs, low in pollution and price, and abundant in raw material resources; interaction of Chinese herbal medicines of spatholobus stem, herba ecliptae, cortex phellodendri, coptis chinensis, fructus rubi, atractylodes macrocephala, peppermint and hemerocallis citrina baroni which contain active ingredients is realized, so that not only is respiratory burst of leucocytes of the groupers improved, but also the leucocyte phagocytosis activity of the groupers is improved, the high average feed coefficient of the groupers is effectively reduced, the low survival rate of the groupers is increased, and the effect of immunopotentiator is excellent.

Provided is technology of external model construction of pseudosciaena crocea head kidney macrophage. The fish head kidney macrophage is horizontally and centrifugally separated by utilizing Percoll with 31% / 45% gradient and is cultured in an L15 compound culture medium under the condition that the temperature is 22 DEG C. Each liter of the culture medium contains 50ml fetal calf serum, 0.1 million UI penicillin / streptomycin, 0.05 million UI heparin, 10mmol 14-ethoxyl-piperazine ethanesulfonic acid (HEPES) and 0.5g glucose. The pH of the culture medium is 7.4. The method and the application have the advantages that (1) the proportion of the pseudosciaena crocea head kidney macrophage which is centrifugally separated by utilizing Percoll with 31% / 45% gradient in the separation cell is as high as 42%; (2) the purity of the adherence pseudosciaena crocea head kidney macrophage after liquid culture is as high as 90%; (3) the livability of the pseudosciaena crocea head kidney macrophage after one week culture is higher than 58%; and (4) a function checking experiment proves that LPS can remarkably affect the swallow function and breath eruption activity of the cultured pseudosciaena crocea head kidney macrophage.

The invention relates to the technical field of feed processing, in particular to a formula and preparing method of an ecological feed for stereoscopic breeding in a sea cucumber, prawn and nettlefishpond. The feed is prepared from 5-25 parts of defatted fish meal, 2-12 parts of scallop skirt, 10-30 parts of corn straw powder, 10-40 parts of bean pulp, 1-10 parts of shrimp head meal, 10-30 partsof wheat meal, 1-10 parts of oyster shell powder, 1-10 parts of a probiotics powder leavening agent, 1-5 parts of a vitamin mixture, 1-5 parts of a mineral mixture, 1-3 parts of monocalcium phosphateand 10-40 parts of water. The feed prepared through the formula and preparing method is high in nutrient transmission efficiency; the feed can be used for rich water, the digestion and absorption rateof the feed is increased compared with a traditional feed, the feed has a substrate improving function, and the respiratory burst, phenol oxidase, superoxide dismutase and catalase in prawn immune indexes are improved.

The invention relates to an application of a cell wall skeleton as a penaeus vannamei feed additive. According to the application, 0.8-1.2g of the cell wall skeleton is added to each kilogram of a penaeus vannamei basal feed, wherein the cell wall skeleton is obtained in the following manner of enabling separated and purified nocardiopsis lucentensis to be subjected to enlarged culture, performingultrasonic wave crushing on fermentation liquor, performing centrifuging, performing enzymolysis with trypsin, performing defatting, and performing freeze drying so as to obtain a white cell wall skeleton. According to the application disclosed by the invention, the activity of superoxide dismutase, nitricoxide synthase, phenoloxidase, lysozyme and glutathione peroxidase in hemolymph and hepatopancreas of penaeus vannamei can be notably increased, the concentration of malondialdehyde can be notably reduced, the respiratory burst activity of the hemolymph of the penaeus vannamei can be notablystrengthened, the immunity protection ratio of the penaeus vannamei can be obviously increased, and the breeding survival rate of the penaeus vannamei can be obviously increased.

To confirm the safety of a fermented papaya preparation in type 2 diabetic patients while determining its in vivo effect on respiratory burst in human peripheral-blood mononuclear cells to find further health advantages for the type 2 diabetic patients. A respiratory burst activity promoter for improving the production of reactive oxygen species (ROS) induced by NADPH oxidase and promoting respiratory burst activity in type 2 diabetic patients, comprising a fermented papaya preparation as an active ingredient.

The present invention relates to a method of treating an inflammatory disorder in a subject with an effective amount of compound having the general formula (I) as described in the present application, under conditions effective to treat the inflammatory disorder. The present invention also relates to a method of inhibiting respiratory burst in neutrophils without inhibiting degranulation in or bacterial killing by the neutrophils by contacting neutrophils with the compounds described above.

A compound Chinese herbal medicine preparation for improving tilapia immunity is obtained by mixing the following components, by weight, 40% of houttuynia cordata, 5% of radix scutellariae, 5% of isatis root, 5% of fructus forsythiae, 10% of honeysuckle, 5% of liquorice, 5% of rheum officinale, 10% of astragalus, 5% of angelica sinensis and 10% of hawthorn. The compound Chinese herbal medicine preparation can remarkably improve activity of superoxide dismutase (SOD) in tilapia serum, the content of nitric oxide (NO), the activity of serum lysoyme (LSZ), phagocytic percentage, phagocytic indexand respiratory burst, greatly reduces the content of serum malondialdehyde (MDA) of tilapia, and effectively improves the tilapia immunity. Feed containing the compound Chinese herbal medicine preparation is further disclosed. The production process is simple, the compound Chinese herbal medicine preparation can be added into commodity feed directly and can not affect taste of the feed, Chinese herbal medicine raw materials used in the preparation are low in price, wide and stable in sources, and the preparation can be produced in industrialization mode.

The invention relates to isopentenyl stilbene and application thereof in preparing medicine for treating inflammatory diseases. The isopentenyl stilbene is a new compound separated from Artocarpus styracifolius Pierre which is a plant of moraceae artocarpus, the compound is named as styracifolius phenol B, and the structure is shown in the description. The compound has strong inhibitory activity on respiratory burst of rat PMNs, the IC50 of the compound is 0.17+ / -0.03 muM, and the compound can be further used for preparing the medicine for treating the inflammatory diseases, and used for clinical treatment of various kinds of oxidative damages such as rheumatoid arthritis, compensatory anti-inflammatory response syndrome, and systemic inflammatory response syndrome caused by excessive activation of the PMNs.

The invention relates to isopentenyl stilbene and application thereof to preparation of drugs for treating inflammatory diseases. The isopentenyl stilbene is a new compound separated from artocarpus styracifolius Pierre which is a moraceae artocarpus plant, is named (+ / -)-artocarpus styracifolius phenol D, and has a structure as shown in the following description. The compound has strong inhibitory activity on rat polymorphonuclear neutrophils (PMNs) respiratory burst, the IC50 is 1.5+ / -0.22 muM, and the compound can be further used for preparing the drugs for treating the inflammatory diseases, and is used in clinical treatment of various oxidative damage, such as rheumatoid arthritis, compensatory anti-inflammatory response syndrome (CARS), and systemic inflammatory response syndrome (SIRS), caused by the excessive activation of PMNs.

The invention relates to an application of a cell wall skeleton as a penaeus vannamei feed additive. According to the application, 0.8-1.2g of the cell wall skeleton is added to each kilogram of a penaeus vannamei basal feed, wherein the cell wall skeleton is obtained in the following manner of enabling separated and purified nocardiopsis lucentensis to be subjected to enlarged culture, performingultrasonic wave crushing on fermentation liquor, performing centrifuging, performing enzymolysis with trypsin, performing defatting, and performing freeze drying so as to obtain a white cell wall skeleton. According to the application disclosed by the invention, the activity of superoxide dismutase, nitricoxide synthase, phenoloxidase, lysozyme and glutathione peroxidase in hemolymph and hepatopancreas of penaeus vannamei can be notably increased, the concentration of malondialdehyde can be notably reduced, the respiratory burst activity of the hemolymph of the penaeus vannamei can be notablystrengthened, the immunity protection ratio of the penaeus vannamei can be obviously increased, and the breeding survival rate of the penaeus vannamei can be obviously increased.

This invention relates to two stilbene derivatives and application thereof to preparation of drugs for treating inflammatory diseases. According to the invention, two stilbene compounds are separatedfrom artocarpus styracifolius Pierre which is a moraceae artocarpus plant; the two stilbene compounds are respectively (+ / -)-artocarpus styracifolius phenol E and (+ / -)-hypargystilbene B. An activitytest result shows that the two compounds provided by the invention have strong inhibitory activity on rat polymorphonuclear neutrophils (PMNs) respiratory burst, and the median inhibitory concentrations are respectively 0.45+ / -0.13 and 5.57+ / -0.24 muM; the two compounds can be further used for preparing the drugs for treating the inflammatory diseases.

The invention belongs to the technical field of fish feeds, and particularly relates to a feed capable of improving immune function and oxidation resistance of grass carps. The fish feed is prepared from the following raw materials of black rice, corn germ meal, oat, a nutrient supplement, fresh cushaw stem leaves, fresh comfrey, konjac gum and creeping oxalis juice. The fish feed is easy to digest and absorb, and can effectively regulate intestinal tract flora balanced, promote immune organs of the grass carps to grow mature, stimulate respiratory burst activity, and improve immunity and oxidation resistance of organisms, so that the disease resistance can be improved, the growth can be promoted. The part of specificity function and non-specificity function of the grass carps can be improved, the local or whole defense function of the organisms can be improved, the nutrient metabolism level can be increased, free radicals can be effectively eliminated, lipid peroxidation is avoided, damage of free radicals on the organisms can be alleviated, the diseases of the grass carps in the breeding process are reduced, the quick and strong growth of the grass carps is effectively promoted,the daily gain is increased, and the economic benefits of breeding households are increased.

The invention relates to isopentenyl toluylene and application thereof in preparing medicine for treating inflammatory diseases. The isopentenyl toluylene is a new compound separated from Artocarpus styracifolius Pierre which is a plant of moraceae artocarpus, the compound is named as (+ / -)-styracifolius phenol A, and the structure is shown in the description. The compound has strong inhibitory activity on respiratory burst of rat PMNs, the IC50 of the compound is 2.62+ / -0.35 muM, and the compound can be further used for preparing the medicine for treating the inflammatory diseases, and used for clinical treatment of various kinds of oxidative damages such as rheumatoid arthritis, compensatory anti-inflammatory response syndrome, and systemic inflammatory response syndrome caused by excessive activation of the PMNs.

The invention relates to a preparation method and application of a prawn non-specific immunity reinforcing agent oligopeptide. The preparation method comprises the following steps of: mincing pacific alaskapollack dogmeat by a meat mincer and then placing in an enzyme reactor to heat and sterilize; triturating and then heating to 90 DEG C to preserve the temperature; sterilizing for 20 minutes in a constant temperature water bath; adding water and protease preparations to mix; carrying out enzymolysis under the condition of optimized enzymolysis process parameters; then heating enzymolysis liquid so that protease is inactivated; roughly filtering fishbones and centrifugalizing the enzymolysis liquid to obtain a soluble protein oligopeptide; ultrafiltering, purifying, freezing and drying and then obtaining protein oligopeptide powder. The protease preparations comprise Alcalase and Flavourzyme, and the enzymolysis process parameters comprise the enzymolysis temperature of 55 DEG C, the initial pH value of 8.5, the material and water ratio of 1:1 and the enzymolysis time of 3 hours. A macromolecule substance is intercepted by using a method for combining 8,000rpm high-speed centrifugalization and 100kDa ultrafiltration membrane ultrafiltrating purification, and 30 percent of protein oligopeptide with the molecular weight of smaller than 100Da is obtained, the further filteration is carried out by 1kDa / 5kDa ultrafiltration membrane to obtain an ultrafiltrate, and the oligopeptide powder is obtained by freezing and drying the ultrafiltrate. The oligopeptide powder replaces 5 percent of fish meal in prawn feed and can obviously improve the blood corpuscle density, the phenoloxidase activity, the respiratory burst activity, the SOD activity, the NOS activity and the specific growing rate of a prawn.

A method for estimating the number of phagocytes in a body fluid of an animal comprises stimulating the NADPH oxidase activity of the phagocytes; and quantifying the reductive deoxygenation of the chemiluminescent substrate by chemiluminescence of the emission of the chemiluminescent substrate using an instrument capable of measuring light. The NADPH oxidase activity of the phagocytes is preferably stimulated by immunity or a chemical substance capable of activating the respiratory burst of the phagocytes. The NADPH oxidase activity of the phagocytes is preferably stimulated by a stimulus in solution or coated onto the surface in contact with the phagocytes. The stimulant is preferably a phorbol myristic acid acetate (PMA). The animal is preferably a human, and the body fluid is preferably blood and / or spinal fluid. The phagocyte is preferably a neutrophil, and the chemiluminescent substrate is preferably a lucigenin (N, N '-dimethyl-9, 9'-bis acridinium dinitrate). A treatment method based on the estimation method is also provided.

The invention discloses a kit for detecting a neutrophile granulocyte respiratory burst function. The kit comprises the following components: a PMA solution, a DHR123 solution, a catalase solution, an equilibrium liquid, washing liquid, a red blood cell lysis buffer and a diluent. The kit provided by the invention can be used for detecting the ability of neutrophile granulocyte on producing active oxygen, and is particularly applicable to clinically screening patients with chronic granulomatous diseases; can also be used for detecting a neutrophile granulocyte respiratory burst function change under other various physiological and pathological conditions, and evaluating the influence of a medicine on the neutrophile granulocyte respiratory burst function. The kit not only can be used for detecting a human sample, but also can be used for other species such as mice, rats, rabbits, dogs, cows and pigs. The kit is mainly based on a DHR123 flow cytometer analytic method, requires less blood volume, and has the advantages of simple operation, fast detection speed, low cost, high popularization performance, good repeatability, accurate and clear detection result, high specificity and easiness for analysis.

This invention relates to two stilbene derivatives and application thereof to preparation of drugs for treating inflammatory diseases. According to the invention, two stilbene compounds are separatedfrom artocarpus styracifolius Pierre which is a moraceae artocarpus plant; the two stilbene compounds are respectively (+ / -)-artocarpus styracifolius phenol E and (+ / -)-hypargystilbene B. An activitytest result shows that the two compounds provided by the invention have strong inhibitory activity on rat polymorphonuclear neutrophils (PMNs) respiratory burst, and the median inhibitory concentrations are respectively 0.45+ / -0.13 and 5.57+ / -0.24 muM; the two compounds can be further used for preparing the drugs for treating the inflammatory diseases.

The invention provides a feed fat composition, and belongs to the technical field of nutrient adjustment and control of aquatic animals. The feed fat composition comprises a basic ration feed and a fat feed, wherein the mass ratio of the basic ration feed to the fat feed is 100 to (6-8), each gram of the feed contains 0.2-0.3g of fish oil, 0.2-0.4g of ballonfish liver oil and 0.4-0.5g of vegetableoil. According to the feed fat composition provided by the invention, the respiratory burst and the phagocytic activity of macrophages of a fish body can be effectively improved, streptococcus is swallowed, the mortality rate after streptococcal infection is reduced, and healthy aquaculture of fish is promoted.

The invention provides agilawood bath foam with antibacterial and anti-inflammatory functions. The agilawood bath foam comprises the following components in parts by weight: 10-18 parts of a first component ketone compound extracting solution, 22-35 parts of a second component lignan compound extracting solution, 2-5 parts of a third component triterpenoid and 20-30 parts of a fourth component butyl avocado oleate. The agilawood bubble bath liquid has the effect of inhibiting neutrophile granulocyte respiratory burst to a certain extent, allergy and some cardiovascular and cerebrovascular diseases can be prevented, and it is found in the test process that the effect can be achieved only by combining the first component, the second component and the third component.

The invention relates to isopentenyl toluylene and application thereof in preparing medicine for treating inflammatory diseases. The isopentenyl toluylene is a new compound separated from Artocarpus styracifolius Pierre which is a plant of moraceae artocarpus, the compound is named as (+ / -)-styracifolius phenol A, and the structure is shown in the description. The compound has strong inhibitory activity on respiratory burst of rat PMNs, the IC50 of the compound is 2.62+ / -0.35 muM, and the compound can be further used for preparing the medicine for treating the inflammatory diseases, and used for clinical treatment of various kinds of oxidative damages such as rheumatoid arthritis, compensatory anti-inflammatory response syndrome, and systemic inflammatory response syndrome caused by excessive activation of the PMNs.

The invention provides a screening method of plant-derived immunoenhancement type feed additive for eels. The screening method comprises steps as follows: preparing a plant-derived extract liquid, andthen, preparing an eel plasma leukocyte solution by a discontinuous density gradient centrifugation method; determining respiratory burst activity and phagocytosis vitality of plasma leukocytes by anELIASA and a flow cytometer respectively, and the activation effect of the plant-derived extract on plasma leukocytes of eels is evaluated in combination of a computation formula. The screening method can complete cell vitality effect tests at high, medium and low concentrations of multiple plant-derived liquids simultaneously at one time, and provides efficient screening technical reference forapplication of the plant-derived extract in aquaculture and fish drug development.

The invention relates to a prenylated flavonoid, and applications thereof in preparing drugs used for treating inflammatory diseases. The prenylated flavonoid is a novel compound separated from artocarpus heterophyllus of Artocarpus, Moraceae, and is named as artoheteroid C. The structure of artoheteroid C is disclosed in the invention, possesses high inhibition activity on mouse neutrophil respiratory burst, can be used for preparing drugs used for treating inflammatory diseases, and can be used for clinical treatment of oxidative damages, such as rheumatoid arthritis, compensatory anti-inflammatory response syndrome, and systemic inflammatory response syndrome, caused by neutrophil excessive activation; and the IC50 value is 7.5<mu>M.