33 results about "Respiratory burst" patented technology

The present invention relates to a method of treating an inflammatory disorder in a subject with an effective amount of compound having the general formula (I) as described in the present application, under conditions effective to treat the inflammatory disorder. The present invention also relates to a method of inhibiting respiratory burst in neutrophils without inhibiting degranulation in or bacterial killing by the neutrophils by contacting neutrophils with the compounds described above.

A compound Chinese herbal medicine preparation for improving tilapia immunity is obtained by mixing the following components, by weight, 40% of houttuynia cordata, 5% of radix scutellariae, 5% of isatis root, 5% of fructus forsythiae, 10% of honeysuckle, 5% of liquorice, 5% of rheum officinale, 10% of astragalus, 5% of angelica sinensis and 10% of hawthorn. The compound Chinese herbal medicine preparation can remarkably improve activity of superoxide dismutase (SOD) in tilapia serum, the content of nitric oxide (NO), the activity of serum lysoyme (LSZ), phagocytic percentage, phagocytic indexand respiratory burst, greatly reduces the content of serum malondialdehyde (MDA) of tilapia, and effectively improves the tilapia immunity. Feed containing the compound Chinese herbal medicine preparation is further disclosed. The production process is simple, the compound Chinese herbal medicine preparation can be added into commodity feed directly and can not affect taste of the feed, Chinese herbal medicine raw materials used in the preparation are low in price, wide and stable in sources, and the preparation can be produced in industrialization mode.

The invention relates to an application of a cell wall skeleton as a penaeus vannamei feed additive. According to the application, 0.8-1.2g of the cell wall skeleton is added to each kilogram of a penaeus vannamei basal feed, wherein the cell wall skeleton is obtained in the following manner of enabling separated and purified nocardiopsis lucentensis to be subjected to enlarged culture, performingultrasonic wave crushing on fermentation liquor, performing centrifuging, performing enzymolysis with trypsin, performing defatting, and performing freeze drying so as to obtain a white cell wall skeleton. According to the application disclosed by the invention, the activity of superoxide dismutase, nitricoxide synthase, phenoloxidase, lysozyme and glutathione peroxidase in hemolymph and hepatopancreas of penaeus vannamei can be notably increased, the concentration of malondialdehyde can be notably reduced, the respiratory burst activity of the hemolymph of the penaeus vannamei can be notablystrengthened, the immunity protection ratio of the penaeus vannamei can be obviously increased, and the breeding survival rate of the penaeus vannamei can be obviously increased.

The invention relates to a stilbene derivative and application thereof to preparation of drugs for treating inflammatory diseases. The stilbene derivative is a new compound separated from artocarpus styracifolius Pierre which is a moraceae artocarpus plant, is named (+ / -)-artocarpus styracifolius phenol C, and has a structure as shown in the following description. The compound has strong inhibitory activity on rat polymorphonuclear neutrophils (PMNs) respiratory burst, the IC50 is 4.24+ / -0.58 muM, and the compound can be further used for preparing the drugs for treating the inflammatory diseases, and is used in clinical treatment of various oxidative damage, such as rheumatoid arthritis, compensatory anti-inflammatory response syndrome (CARS) and systemic inflammatory response syndrome (SIRS), caused by the excessive activation of PMNs.

The invention relates to a preparation method and application of a prawn non-specific immunity reinforcing agent oligopeptide. The preparation method comprises the following steps of: mincing pacific alaskapollack dogmeat by a meat mincer and then placing in an enzyme reactor to heat and sterilize; triturating and then heating to 90 DEG C to preserve the temperature; sterilizing for 20 minutes in a constant temperature water bath; adding water and protease preparations to mix; carrying out enzymolysis under the condition of optimized enzymolysis process parameters; then heating enzymolysis liquid so that protease is inactivated; roughly filtering fishbones and centrifugalizing the enzymolysis liquid to obtain a soluble protein oligopeptide; ultrafiltering, purifying, freezing and drying and then obtaining protein oligopeptide powder. The protease preparations comprise Alcalase and Flavourzyme, and the enzymolysis process parameters comprise the enzymolysis temperature of 55 DEG C, the initial pH value of 8.5, the material and water ratio of 1:1 and the enzymolysis time of 3 hours. A macromolecule substance is intercepted by using a method for combining 8,000rpm high-speed centrifugalization and 100kDa ultrafiltration membrane ultrafiltrating purification, and 30 percent of protein oligopeptide with the molecular weight of smaller than 100Da is obtained, the further filteration is carried out by 1kDa / 5kDa ultrafiltration membrane to obtain an ultrafiltrate, and the oligopeptide powder is obtained by freezing and drying the ultrafiltrate. The oligopeptide powder replaces 5 percent of fish meal in prawn feed and can obviously improve the blood corpuscle density, the phenoloxidase activity, the respiratory burst activity, the SOD activity, the NOS activity and the specific growing rate of a prawn.

The invention relates to a prenylated flavonoid, and applications thereof in preparing drugs used for treating inflammatory diseases. The prenylated flavonoid is a novel compound separated from artocarpus heterophyllus of Artocarpus, Moraceae, and is named as artoheteroid C. The structure of artoheteroid C is disclosed in the invention, possesses high inhibition activity on mouse neutrophil respiratory burst, can be used for preparing drugs used for treating inflammatory diseases, and can be used for clinical treatment of oxidative damages, such as rheumatoid arthritis, compensatory anti-inflammatory response syndrome, and systemic inflammatory response syndrome, caused by neutrophil excessive activation; and the IC50 value is 7.5<mu>M.