Feed fat composition
A technology of feed and oil, applied in the field of nutritional regulation of aquatic animals, to achieve the effects of promoting healthy fish farming, reducing mortality, and increasing phagocytic activity
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preparation example Construction
[0027] In the present invention, the source of the fish oil and puffer fish liver oil is not particularly limited, and conventional commercially available products can be used. In the present invention, the preparation method of the puffer fish liver oil preferably includes: collecting fresh puffer fish liver tissue, placing it in a freeze dryer, and freezing and extracting at -40°C for 48 hours, when the oil yield is 65% and the oil is yellow , collect fugu liver oil. In the present invention, the collected puffer fish liver oil is preferably placed in refrigerated storage at 4°C. In the present invention, the fugu liver oil contains C16:0 and C18:1, the contents of which are 21.049% and 29.111% respectively, which help to promote the infiltration of macrophages, enhance the respiratory burst and phagocytic activity of macrophages, and Make macrophages produce a large amount of inflammatory factors IL-1β and TNF-α, and enhance the stress regulation ability of fish after stre...
Embodiment approach
[0034] A set of feed oil addition and its implementation method for improving the respiratory burst and phagocytic activity of head kidney macrophages after Streptococcus tilapia infection include the following steps:
[0035] Step 1, preparation of basal ration and feed: 200 g of Gifu tilapia were selected, and 4 groups of rations and feed (protein level of 29%) without added oil feed were prepared. Select 12 experimental ponds of 1 mu, place 2000 tilapias in each experimental pond, and set 3 parallel groups in each experimental group;
[0036] Step 2, the composition and ratio of oil and fat feed.
[0037] The first group: the fat composition includes: fish oil, puffer fish liver oil and soybean oil, by weight, every 1g of fat feed contains 0.2g of fish oil, 0.3g of puffer fish liver oil, and 0.5g of soybean oil;
[0038] The second group: oil composition includes: fish oil. In parts by weight, every 1g of feed oil contains 1g of fish oil;
[0039] The third group: oil co...
Embodiment 2
[0054] A set of feed oil addition and its implementation method for improving the respiratory burst and phagocytic activity of head kidney macrophages after Streptococcus tilapia infection include the following steps:
[0055] Step 1. Before the arrival of the high temperature season, six 2-mu tilapia culture ponds in Gaozhou, Maoming, Guangdong were selected for one-month immunity promotion. The size of the experimental fish is about 400g. It has been cultured for 90 days, and the stocking density is 2500 fish / mu.
[0056] The 2nd step, experimental group 1: continue to throw something and feed commercial fish feed of tilapia, protein content 28%, fat is 6%, wherein, comprise fish oil 0.5g and soybean oil 0.5g in every 1g feed fat; Experimental group 2: remove Except for oil and fat, the composition of other feeds remained unchanged, and 1 g of feed fat contained fish oil, puffer fish liver oil and soybean oil. In parts by weight, every 1g of feed fat contains 0.2g of fish o...
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