95 results about "Hemolymph" patented technology

The invention discloses an artificial medium of trichogramma. The artificial medium comprises the following ingredients in mass fraction: 19-40% of insect pupae hemolymph, 17-38% of egg yolk, 10-33% of milk, 4-19% of a Nissl salt solution, 0.8-8% of trehalose, 2-15% of a ganoderan solution with a mass fraction of 5% and 2-15% of a chitosan oligosaccharide solution with a mass fraction of 3.6%. By adopting the artificial medium of trichogramma, the breeding effect of trichogramma is obviously better than that of a traditional artificial medium through the trehalose, the ganoderan solution and the chitosan oligosaccharide solution via effective composition, and the content of insects can be reduced, so that the breeding cost of trichogramma is reduced. Through the artificial medium of trichogramma, trichogramma can be bred by subculturing, no degradation phenomenon exists, and the breeding effect is stable. By adopting the artificial medium of trichogramma, various kinds of trichogramma such as trichogramma dendrolimi, trichogramma confusum, Asian corn borer trichogramma, European corn borer trichogramma and geographical populations thereof can be bred, and good effects are realized.

The invention relates to the field of biotechnology, and particularly relates to the bioactivity of the insect, insect hemolymph and assembly active substance thereof in activating a prophenoloxidase activation system or/and Toll signal transmission path system and an application of the insect, insect hemolymph and assembly active substance thereof in microorganism and related molecular mode detection thereof, preparation of the assembly active substance of prophenoloxidase activation system and separation and purification of antibacterial peptide. The invention also provides an insect hemolymph collection method and the storage method and conditions of the hemolymph; under the conditions, the stability of the insect hemolymph is better, and the functions of the insect hemolymph can be better played.

Each 100 ml of blood anticoagulant contains trisodium citrate 3.31-3.32g, citric acid 0.01-0.05g, glucose 2.45-3.37g and the rest is water. The tests show that after the external blood is undergone the process of anticoagulation treatment it can prolong the stroage life of lymphocyte to 48-72 hr. from original 4-6 hr. Said invention also provides its application method.

The invention discloses application of glycine or a glycine metabolism regulator, i.e., 1-aminocyclopropane-1-carboxylic acid (ACPC), in improving domestic silkworm silk output, as well as a method for improving the domestic silkworm silk output by supplying exogenous glycine or ACPC to domestic silkworms in a larval period. According to the invention, by researching change in a hemolymph metabolite spectrum of different varieties of domestic silkworms having a large difference in silk output based on GC-MS metabonomics technology, strong correlation is proved to exist between the glycine (42.8% of silk fibroin), as an important component, of domestic silkworm silk fibroin, and a cocoon layer ratio (correlation coefficient is -0.892); the domestic silkworm silk output is significantly improved by intervening metabolism of the silkworms in the larval period through in vitro administration of the glycine or the glycine ACPC; therefore, the invention not only widens application of the glycine or the ACPC in the field of biology, but also provides a new method for improving the silk output in silkworm industry.

The invention discloses a pteromalus puparum venom Kazal-type serine protease Inhibitor PpSPI20 protein. The protein comprises an amino acid sequence shown as SEQ ID NO: 2. The invention further discloses genes coding the pteromalus puparum venom Kazal-type serine protease Inhibitor PpSPI20 protein. The genes comprise a nucleotide sequence at sites 70-246 in SEQ ID NO:1. The inhibitor disclosed bythe invention can be used for inhibiting activation of hemolymph PPO (Poly Phenylene Oxide) of imported cabbageworms or papilio xuthus. The invention further discloses a method for improving prevention ability of brassicaceous vegetables on lepidoptera pests. The method comprises the following steps: transforming brassicaceous vegetable cells by using the genes comprising the nucleotide sequenceshown as SEQ ID NO:1, and cultivating the transformed brassicaceous vegetable cells into plants.

The invention provides an artificial intelligence identification method for a peripheral hemolymph micronucleus cell image, and the method comprises the steps: collecting a to-be-analyzed sample through a collection device, and obtaining a cell image; carrying out segmentation processing on the obtained cell image to obtain a foreground to-be-detected area; identifying, classifying and counting the cells on the foreground to-be-detected area; storing the cell type information on the foreground to-be-detected area and the position information of the foreground to-be-detected area on the cell image to form traceable information; and forming an image-text report according to the traceable information, and storing the image-text report as an artificial intelligence system learning training material. According to the artificial intelligence identification method for the peripheral hemolymph micronucleus cell image provided by the invention, automatic identification and classification of specific images of lymphoblasts, micronucleus cells and nude nuclei of peripheral blood can be realized. Compared with an existing microscope artificial microscopic examination method, the statistical accuracy is higher, and the detection speed is remarkably increased.

The invention relates to a preparation method of silkworm decellularization hemolymph liquid. The preparation method comprises a series of steps of disinfecting the body surfaces of silkworms, preparing the hemolymph liquid of the silkworms, carrying out antioxidant treatment and cell extraction on the liquid, removing bacteria from the liquid and the like. The method takes the hemolymph liquid of the silkworms as materials to prepare a nutrient substance applied to the cell culture of insects. Vitamin C is used for inhibiting the activity of tyrosine oxidase in the hemolymph liquid, so that the hemolymph liquid does not get black by oxidation when being contacted with the outside, the composition and the content of main nutrient substances in the hemolymph liquid are not changed, and the cell culture is not influenced. Compared with serum, the hemolymph liquid of the silkworms is more suitable for the cell culture of the insects, and is low in price.

The invention discloses a method for constructing transgenic Nosema bombycis, characterized by injecting a transgenic vector wrapped by liposome in body cavity of the Bombyx mori infected with Nosema bombycis, after 2-4 days, adding silkworm hemolymph in Bombyx mori BmN culture cells to cultivate; when microsporidian presents green fluorescent light, using an insect medium to extremely dilute and adding into a cell culture plate, selecting a culture pore where only one culture cell presents green fluorescent light, adding normal culture cells to culture for 2-4 days to obtain infected culture cells; repeating for at least three times, taking the obtained infected culture cells, conducting homogenate and crushing the cells, conducting differential centrifugation, purifying microsporidian, infecting the Bombyx mori, after the Bombyx mori falls ill, purifying the infected tissues to obtain the transgenic Nosema bombycis. According to the invention, exogenous genes can be integrated into the Nosema bombycis genome, the modification of Nosema bombycis by gene engineering is realized, the adjustment of expression of the microsporidian genes is realized, and a method for microsporidian gene function research is provided.

The invention belongs to the technical field of plant poison toxicity evaluation, and provides a metabonomics-based research method for a toxicity effect of jujube flower nectar on honeybees, aiming at the current situation that the toxicity effect of the jujube flower nectar on the honeybees cannot be explained in the existing research. The research method comprise the steps of: collecting honeybees with jujube flower diseases and taking healthy honeybees as a contrast; collecting hemolymph of the two groups of honeybees; combining a UPLC-Q-TOF MS technology with multi-dimensional statisticalanalysis to carry out full spectrum analysis on the sample to obtain primary mass spectrum data and secondary mass spectrum data; adopting xCMS software to carry out peak extraction and metabolite identification on the data, screening metabolite targets and metabolic profiles related to honeybee jujube flower diseases from metabolite, tracking and screening of pathogenicity of jujube flower nectar on honeybees under the guidance of metabolite target analysis and metabolic profile analysis, and illustrating a toxic action mechanism of the jujube flower nectar on honeybees. The research methodhas the advantages of being low in cost, easy to operate, rapid in detection, high in sensitivity, strong in selectivity, good in repeatability, comprehensive in obtained information and the like.

The invention belongs to the technical field of freshwater aquaculture, and relates to a method for analyzing aeromonas hydrophila infected macrobrachium nipponense hemocyte differential expression protein based on a proteomics quantitative technology. The method comprises the following steps of (1) extracting the hemolymph of macrobrachium nipponense infected with aeromonas hydrophila, separatingthe blood cells, and cracking the cells to extract protein; (2) carrying out trypsin enzymolysis on the protein obtained in the step (2), and carrying out iTRAQ labeling on an enzymolysis peptide fragment; (3) carrying out purification, liquid chromatographic separation and mass spectrometric analysis on the peptide fragment marked in the step (3); and (4) carrying out protein identification, quantitative analysis and bioinformatics analysis on the mass spectrum analysis result obtained in the step (3), and analyzing to obtain differentially expressed protein. The invention is based on the rapid proteomics research of the infected macrobrachium nipponense by iTRAQ, and provides a reference for further researching the effect of immune-related proteins.

The invention discloses a pteromalus puparum venom Kazal-type serine protease inhibitor protein PpSPI24 with an amino acid sequence as shown in SEQ ID NO:2. The invention further discloses a gene forcoding the chrysalis nasonia venom Kazal-type serine protease inhibitor protein PpSPI24, and the gene has nucleotide sequences of sites 70-231 in SEQ ID NO:1. The inhibitor can be used for inhibitingactivation of imported cabbageworm or papilio xuthus linnaeus hemolymph PPO. The invention further discloses a method of improving prevention force, on lepidoptera pests, of brassicaceous vegetables,and the method comprises the following steps: converting cells of the brassicaceous vegetables by the gene with the nucleotide sequences as shown in SEQ ID NO:1; and cultivating the converted cells ofthe brassicaceous vegetables into plants.

A method for simply and rapidly detecting oyster ploidy is disclosed. The method includes firstly crumbing shell edges, of two shells of an oyster, close to the adductor muscle with sharp-nose pliersto form a gap having a length of 2-3 mm; drawing hemolymph by inserting a disposable syringe through the gap into a hemolymph sinus of the adductor muscle; after the hemolymph is immobilized with absolute alcohol, performing centrifugation and removing a liquid supernatant; adding PBS and suspending the mixture again; performing RNA enzyme reaction and PI staining in order; and performing filtration with a cloth sieve having a size of 300 meshes; and then detecting the DNA content with a flow cytometer to determine the ploidy. The method performing ploidy detection through drawing the hemolymph in the hemolymph sinus of the adductor muscle of the oyster is non-lethal, simple, rapid, not limited by oyster living environment, and the like, and is of great significance for oyster polyploidy breeding research, especially ploidy detection of live oyster polyploidy and subsequent deep research.

The invention discloses a preparation method of an apis cerana antimicrobial peptide AccRoyalisin polyclonal antibody and the use thereof. Apis cerana royal jelly antimicrobial peptide AccRoyalisin recombined and expressed by escherichia coli is taken as an antigen, and a big rabbit is immunized to prepare a polyclonal antibody, the valence of the antibody is larger than 40,000 times, the polyclonal antibody is taken as a primary antibody, recombined and expressed GST-AccRoyalisin is taken as a standard protein antibody to perform doubling dilution, the absorbance of GST-AccRoyalisin protein samples with different concentrations is detected through an Elisa method by a microplate reader, and the standard curve between the absorbance and the MRJPI protein concentration is established. Different bee species or different bee swarms of hemolymph and lyophilized royal jelly powder serve as antibodies after being diluted, and the absorbance of the obtained sample is detected through adopting the Elisa method, and is introduced into the established standard curve to calculate the content of Royalisin of the obtained sample. The invention provides the new use for the Royalisin antibody, and provides a novel quick, qualitative and quantitative detection method for evaluating premunition of the bee species and bee swarms in the bee-keeping field, breeding the antiviral bee swarms and evaluating the antiseptic and antibiosis performance of royal jelly.

The invention relates to the technical field of biological control, in particular to application of nativo to control over filial generations of brown planthopper. Native water dispersible granule (with 75 % mass percent of active ingredients including 25 % mass percent of trifloxystrobin and 50% mass percent of tebuconazole) is utilized by the invention for control over filial generations of brown planthopper.The most salient feature of the invention is described in that nativo, as bactericide, helps to prevent of inhibit yeast-like endosymbiote from entering ovarian tissues from hemolymph of female adults of brown planthopper such that a genital system of brown planthopper is affected and the egg laying amount is reduced; and the hatching rate is greatly decreased because produced eggs lack symbiotic bacteria so that generation of the filial generations of brown planthopper is effectively controlled.

The invention discloses a method for a direct polymerase chain reaction (PCR) adopting litopenaeus vannamei hemolymph. The method comprises the steps of 1, anticoagulant preparation; 2, hemolymph collection and pretreatment; 3, PCR treatment. The method has the advantages of being easy to operate, low in cost, safe and free of toxins, the complex step of extracting DNA is omitted, harm to parent prawns is avoided, and the modern biological requirements are met.