181 results about "Protein identification" patented technology

The invention relates to the technical field of electro-analytical chemistry and protein identification transducers, and discloses a synthesizing method of functionalized ionic liquid and a preparation method and application of a molecular imprinting electrochemical transducer composed of the functionalized ionic liquid, carboxylation multiwalled carbon nanotubes and glassy carbon electrodes. The preparation method of the molecular imprinting electrochemical transducer comprises the steps that the polymerizable amino-functionalized ionic liquid is used as a functional monomer, BSA is used as template protein, N, N'-methylene bisacrylamide is used as a cross-linking agent, an oxidation-reduction system composed of ammonium persulfate and TEMED is used as an initiator, after polymerization, a molecularly imprinted polymer film is formed on the surfaces of the glassy carbon electrodes decorating the carboxylation multiwalled carbon nanotubes, and then the template protein is eluted to obtain the molecular imprinting electrochemical transducer which can identify template protein in a specific mode. The molecular imprinting electrochemical transducer has the advantages of being simple in preparation, low in material cost, high in selectivity, good in biocompatibility, and capable of being used for identifying and detecting protein in aqueous solution.

A simple and quick protocol for chemical treatment, enzymatic or chemical digestion, and subsequent identification of proteins on protein chip arrays is disclosed, together with kits therefor. The chemical treatment comprises denaturation, reduction and alkylation while enzymatic digestion encompasses deglycosylation, dephosphorylation, and digestion by various proteases. Digestion is also accomplished by various chemicals that are known to induce proteolysis. All reactions are carried out sequentially on chip. Subsequent peptide mass fingerprinting or product ion searches allow the identification of specific peptides which can be correlated to proteins. The method of the present invention can be applied to the analysis of biological samples such as urine and plasma to identify biomarkers in diseased states. The methods of the present invention allow complete on chip treatment, which can be used for rapid protein identification and structural characterization of heavily posttranslationally modified proteins.

An algorithm-based system and method for tandem mass spectrometry data acquisition in which multiple precursor ion attributes, such as mass, intensity, mass-to-charge ratio and charge state, as well as results from previously performed mass spectrometry scans, are used to determine the likelihood of identification for each precursor ion. This information is then used to prioritize subsequent tandem mass spectrometry events, such as which precursor ions are to be fragmented and undergo further mass spectrometry analysis. By interrogating precursor ions in order of probability of successful identification, an increase in identified proteins and peptides is achieved.

The invention relates to a large-scale distributed parallel acceleration method and system for protein identification. The method comprises the following steps of: by a parallel processing method, theoretically digesting a protein sequence to obtain peptide sequences, and sorting and redundantly processing the peptide sequences so as to create a peptide index file block; by the parallel processing method, sorting mass spectra spectrums, and evenly dividing the sorted mass spectra spectrums into a plurality of spectrum data blocks; and evenly distributing the spectrum data blocks to a plurality of host processes, and each host process sorting the distributed spectrum data blocks and sequentially assigning to idle slave processes for peptide spectrum matching identification; and summarizing identification results by the parallel processing method, inferring the corresponding protein sequences by the peptide sequences obtained by identification, and generating output files. When a processor has hundreds or even thousands of cores, the satisfactory acceleration efficiency can be obtained when the protein identification is carried out.

Methods and compositions relating to Alzheimer's disease are provided. Specifically, proteins that are differentially expressed in the Alzheimer's disease state relative to their expression in the normal state are provided. Proteins associated with Alzheimer's disease are identified and described. Methods of diagnosis of Alzheimer's disease using the differentially expressed proteins are also provided, as are methods for the identification and therapeutic use of compounds for the prevention and treatment of Alzheimer's disease.

The invention discloses a wild ginseng protein extracting method suitable for dielectrophoresis. The method comprises the following steps of: taking wild ginseng as an experimental material, fully grinding the wild ginseng in liquid nitrogen and transferring the obtained product to a centrifuge tube; adding acetone solution precooled at the temperature of -20 DEG C into the centrifuge tube, washing sample powder for 3 times, collecting sediments by centrifugation and drying the obtained product at room temperature for 20min to obtain the crude extract of the wild ginseng protein; dissolving the crude extract of the wild ginseng protein into lysis buffer, and performing ultrasonic cell disintegration and extracting the supernate by centrifugation to obtain the high-quality solution of the wild ginseng protein. The wild ginseng protein extracting method for the dielectrophoresis is simple and fast, can obtain an electrophoresis pattern having a high repeatability and resolution, is favorable for subsequent protein identification by mass spectrographic analysis and provides a technical support for the proteomic research of the wild ginseng.

The invention discloses a polypeptide mixture isoelectric focusing separation method for proteomics analysis. The method comprises the following steps: performing restriction enzyme digestion on a total protein mixture of a biological sample completely to obtain relatively short peptide fragment mixtures; re-dissolving the peptide fragment mixtures by using a peptide fragment isoelectric focusing buffer solution; performing isoelectric focusing electrophoresis in a loading manner by adopting a loading cup, so as to realize the effective separation of a complex peptide fragment mixture; performing desalination on a taken polypeptide mixture pre-separated from each groove and performing mass spectrometry analysis so as to obtain more polypeptide signals, higher peptide fragment covering rate and higher protein identification number compared with those obtained when a conventional method is adopted. The method is precise, efficient and low in cost, can be used in multidimensional liquid chromatography-mass spectrum identification technology systems of total proteins of the various biological samples, can replace a conventional ion exchange chromatography pre-separation method so as to realize the efficient pre-separation of the peptide fragment mixtures, and avoids the lost of peptide fragments, and therefore, proteome expression profile information with the high covering rate can be established.

The invention relates to a method and kit of particularity recognition and mass spectrum sequencing of protein N-end peptide section. The method and kit can be used for particularity recognition of protein N-end peptide section, increase determinacy and reliability of identification for protein N-end peptide section, and be adapted for protein identification and N-end analysis in biology, medicine and medicament research and application field.

The invention provides a simple and quick protocol for chemical treatment, enzymatic or chemical digestion, and subsequent identification of proteins or polypeptides on a protein chip such as protein chip arrays. The chemical treatment includes denaturation, reduction and alkylation while the enzymatic digestion includes deglycosylation, dephosphorylation, and digestion by various proteases. The proteins or polypeptides can also be digested by using various chemicals that are known to induce proteolysis. All reactions are carried out sequentially on chip. Subsequent peptide mass fingerprinting or product ion searches allow the identification of specific peptides, which can be correlated to proteins. The methods of the present invention can be used to analyze biological samples such as urine and plasma and to identify biomarkers in diseased states. The methods of the present invention allow complete on chip treatment, which can be used for rapid protein identification and structural characterization of heavily posttranslationally modified proteins.