Systems, kits, and methods for detecting and quantifying proteomic activity using
DNA-encoded probes are provided, where the proteomic activity may be enzymatic activity or ligand binding affinity. Such systems and methods
encode quantitative proteomic activity information into
DNA sequence populations and utilize
DNA-linked substrates or ligands as activity probes. The systems, kits, and methods that are directed to detecting ligand affinity further include crosslinking steps to ensure the integrity of the DNA-linked ligands during purification and washing.
Signal detection involves the chemical manipulation of a probe
population downstream of sample
exposure and application of purifying, selective pressure for desired products. Selection-induced changes in DNA abundance between the initial
pool and the purified
pool indicate sample activity.