Application of anti-PDCD4 (Programmed Cell Death 4) antibody in preparation of detection reagent for predicting personalized medicine sensitivity of paclitaxel or derivative drug of paclitaxel
A detection kit, paclitaxel technology, applied in the direction of biological material analysis, measuring devices, instruments, etc., can solve the problems of unclear specific inhibitory targets, and achieve simple and easy detection methods, good reproducibility of results, and significant economic benefits and the effect of social benefits
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Embodiment 1
[0042] Embodiment 1: the relationship between the expression level of tumor suppressor PDCD4 and paclitaxel drug sensitivity
[0043] Cell lines: Human ovarian cancer cell line UACC-1598 was donated by Dr. Guan Xinyuan, Department of Clinical Oncology, University of Hong Kong; human cervical cancer cell line HeLa and human osteosarcoma cell line U2-OS were purchased from Shanghai Cell Bank, Chinese Academy of Sciences; human colon cancer cell line HT -29 was purchased from the American Type Culture Collection (ATCC). at 37°C, 5% CO 2 Under these conditions, UACC-1598 and HeLa cells were cultured in RPMI-1640 medium containing 10% fetal bovine serum; HT-29 cells were cultured in DMEM high-glucose medium containing 15% fetal bovine serum; U2-OS cells were cultured in Cultured in MDEM medium containing 10% fetal bovine serum.
[0044] The main biochemical reagents required:
[0045] Opti-MEM medium (Sigma company)
[0046] Polyethylenimine transfection reagent (Polysciences) ...
Embodiment 2
[0100] Example 2: Detection of PDCD4 protein expression level in clinical lung cancer tissue by immunoblotting method and its guiding significance to paclitaxel individualized drug use
[0101] 1. Lung cancer tissue acquisition: Frozen samples of lung cancer and paracancerous tissues from the Department of Thoracic Surgery, Affiliated Hospital of Harbin Medical University in 2010 were selected, including 77 lung cancer tissues and paracancerous tissues, including 56 males and 21 females. All the collected tissues were diagnosed by experienced pathologists, and no lymphatic metastasis or metastasis of adjacent tissues and organs was found. The age of lung cancer patients in this group ranged from 36 to 75 years old, with an average age of 60 years; there were 26 cases of adenocarcinoma, 42 cases of squamous cell carcinoma and 9 cases of others. Seventy-seven patients with lung cancer received paclitaxel combined with adjuvant chemotherapy. The tissue samples used in this exper...
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