126 results about "Pancreatic enzymes" patented technology

The present invention refers to new microspheres including pancreatic enzymes, pharmaceutical compositions containing them, and a process to obtain them. The process here described doesn't involve the use of solvents and proves to be remarkably shorter and efficient than the producing methods of the prior arts. The microspheres obtained, including one or more pancreatic enzymes, one or more hydrophilic low-melting polymers and eventual excipients, have an high enzymatic activity, bio-availability and stability.

A method for treating a Parkinson's patient with digestive / pancreatic enzymes involves administering an effective amount of digestive / pancreatic enzymes to an individual having the disorder in order to improve a symptom of the disorder. In addition, a method is provided for determining whether an individual has, or may develop, Parkinson's disease or related dysautonomic disorders and for determining whether an individual will benefit from the administration of pancreatic / digestive enzymes to treat the dysautonomic disorder.

A therapeutic agent for the treatment of toxemia, preeclampsia and eclampsia and the method for preparing the therapeutic agents is disclosed. The therapeutic agent is a stable pharmaceutical preparation containing, but not limited to, digestive / pancreatic enzymes. The therapeutic agent may be manufactured by a variety of encapsulation technologies. Delivery of the therapeutic agent may be made orally, through injection, by adherence of a medicated patch or other method. Further, a method of using of a biomarker, the presence of chymotrypsin in the maternal GI tract to determine the likelihood of developing preeclampsia, pregnancy induced hypertension, and eclampsia / toxemia is disclosed.

A stable preparation of digestive / pancreatic enzymes which can be readily formed into a dosage formulation is provided as a treatment of pancreatic insufficiency in persons having cystic fibrosis. The dosage formulation can be administered either by an oral preparation including, but not limited to, a microcapsule, mini-capsule, time released capsule, sprinkle or other methodology. A further object of this invention is to provide a stabilized preparation of a combination medicant which resists degradation by light, heat, humidity or association with commonly used excipients.

A method for treating a Parkinson's patient with digestive / pancreatic enzymes involves administering an effective amount of digestive / pancreatic enzymes to an individual having the disorder in order to improve a symptom of the disorder. In addition, a method is provided for determining whether an individual has, or may develop, Parkinson's disease or related dysautonomic disorders and for determining whether an individual will benefit from the administration of pancreatic / digestive enzymes to treat the dysautonomic disorder.

A therapeutic agent for the treatment of the symptoms of addiction and the method for preparing the therapeutic agent is disclosed. The therapeutic agent is a stable pharmaceutical preparation containing, but not limited to, digestive / pancreatic enzymes. The therapeutic agent may be manufactured by a variety of encapsulation technologies. Delivery of the therapeutic agent may be made orally, through injection, by adherence of a medicated patch or other method. Further, a method of using of a biomarker, the presence of chymotrypsin in the gastrointestinal tract to determine the presence of symptoms of addiction, and the likelihood of relapsing into addiction is disclosed.

The invention provides a chicken bone protein zymolyte and a chicken essence substrate prepared by the chicken bone protein zymolyte, belonging to the technical field of poultry processing. The invention solves the problems of high cost for producing a chicken essence substrate, limited raw material sources, unavailable effective utilization generally by discarding leftovers, such as chicken bone, and the like, and the like in the prior art. In the invention, a novel chicken essence substrate is developed by adopting the cheap leftovers, namely chicken bone as a raw material and utilizing a biological enzymolysis technology and a hot reaction spice research technology; the chicken essence substrate is prepared by the following steps of: adopting the chicken bone zymolyte obtained after the chicken bone is subjected to double enzymolysis by pancreatic enzyme and Flavourzyme composite flavor protease as a main material, mixing the main material with hydrolyzed vegetable protein (HVP), a yeast extract, glucose, xylose, thiamine, chicken oil and propylene glycol according to a certain proportion; and carrying out Maillard reaction in a high-pressure reactor to prepare the chicken essence substrate with vivid chicken fragrance. The chicken essence substrate has vivid chicken fragrance, rich and strong chicken fragrance and long aftertaste.

A therapeutic agent for the treatment of the symptoms of addiction and the method for preparing the therapeutic agent is disclosed. The therapeutic agent is a stable pharmaceutical preparation containing, but not limited to, digestive / pancreatic enzymes. The therapeutic agent may be manufactured by a variety of encapsulation technologies. Delivery of the therapeutic agent may be made orally, through injection, by adherence of a medicated patch or other method. Further, a method of using of a biomarker, the presence of chymotrypsin in the gastrointestinal tract to determine the presence of symptoms of addiction, and the likelihood of relapsing into addiction is disclosed.

A therapeutic agent for the treatment of toxemia, preeclampsia and eclampsia and the method for preparing the therapeutic agents is disclosed. The therapeutic agent is a stable pharmaceutical preparation containing, but not limited to, digestive / pancreatic enzymes. The therapeutic agent may be manufactured by a variety of encapsulation technologies. Delivery of the therapeutic agent may be made orally, through injection, by adherence of a medicated patch or other method. Further, a method of using of a biomarker, the presence of chymotrypsin in the maternal GI tract to determine the likelihood of developing preeclampsia, pregnancy induced hypertension, and eclampsia / toxemia is disclosed.

Compositions of the present invention, comprising the combination of enterically coated and uncoated pancreatic enzyme-containing beads are useful for treating or preventing pancreatitis pain, and optionally disorders associated with digestive enzyme deficiencies.

The invention relates to a method for preparing meat flavor by using vegetable proteins, which comprises the following steps of: (1) carrying out pressure cooking on soybean meal for 20-40 min at a pressure of 0.05 MPa so as to carry out curing processing; (2) hydrolyzing the obtained object for 24 h at a temperature of 50 DEG C by using 1-2% of compound pancreatic enzyme; (3) adding 6% of glucose, 0.8% of L-cysteine and 0.4% of DL-methionine, and carrying out reaction on the obtained product for 60min at a temperature of 115 DEG C; and (4) carrying out filtration and concentration on the obtained product. Vegetable proteins can be soybean meal, corn proteins and peanut cakes, and the like. The composite pancreatic enzyme is composed of 60% of pancreatic enzymes and 40% of papain. According to the invention, meat flavor which produces intense and coordinated aromas can be prepared; and an intensive processing way of vegetable proteins is broadened.

The invention provides a test method for induced differentiating human embryo stem cells to retina pigment epithelial cells, which comprises the following steps that: A) 0.25% pancreatic enzyme and 0.1mg / mL collagenase iv are mixed into phosphate buffered saline (PBS) solution containing 1mM calcium chloride (CaCl2) and 20% KSR, the solution is used for digesting embryo stem (ES) clones, so the ES becomes an ES cell group with 5 to 10 cells; B) the ES clone is incubated in a culture dish which is wrapped by gelatin in a short time so as to remove the feeder layer cells; and C) the ES cell group is cultured in a continuous differential culture medium with the concentration of 6.7*102 cell groups per liters in a non-adhesive bacteria culture dish. The test method has the advantages such as simple and convenient method, good repeatability, high successful rate, good omnisexuality, rich and economical source and the like.

The present invention relates to a small particle size composition comprising pancreatin containing digestive enzymes for use in patients in need, including pediatric, geriatric, and adult patients, particularly those patients with dysphagia or wherein enteral administration using such composition would be suitable. In addition, the invention is directed to the composition as particles, such as micropellets or microgranules having a high potency, high useable yield and at least 10%-90% of 400-800 μm. Furthermore, the composition optionally has an improved enteric coating and concomitant improved stability and enzyme activity compared to conventional prepared enterically coated pancreatic enzyme particles.

The invention relates to a method for preparing human amniotic membrane epithelial cells from human placenta amnion and application thereof, in particular to the method for preparing human amniotic membrane epithelial cells from the placenta amnion. The method comprises the following steps that the surface of a placenta is flushed with a basic balanced salt solution repeatedly and the placenta is sterilized; a foetal membrane outer layer amnion is torn and taken into a glass dish by surgical forceps, the grimy blood on the surface of the foetal membrane outer layer amnion is flushed with the basic balanced salt solution, and then the foetal membrane outer layer amnion is sheared into small blocks; the small amnion blocks are evenly attached to a culture dish, the epithelial layer of the amnion faces downwards, and after drying, a complete medium is added for culture; fluid infusion is conducted two days later, and then culture is continued; when epithelial cells are seen to crawl out, a tissue is removed, and medium change is conducted to continue culture; after multiple typical epithelial cell masses occur, the local fusion rate reaches 90%, and continuous cell culture is conducted; and harvesting is conducted, specifically, digestion is conducted by pancreatic enzymes, counting and freezing storage are conducted, and the human amniotic membrane epithelial cells are obtained. The invention further relates to the prepared human amniotic membrane epithelial cells and the application thereof. The method has the advantages showing in the specifications.

The invention discloses a method for preparing gene recombinant human superoxide dismutase liposome enteric-coated capsules, which prepares Cu-Zn superoxide dismutase liposome enteric-coated capsules by embedding human Cu-Zn superoxide dismutase (specific activity: 18000U / mg) obtained by the fermentation of engineering bacteria into a liposome. The liposome serving as embedding material adopts the mucosa to absorb the phosphatide and cholesterol of an enhancer, the vitamin E of an antioxidant is added into the liposome, and the enteric-coated capsule which can prevent liposome pharmaceuticals from decomposition and destruction by gastric juice is adopted to avoid the destruction of the medicine, so the gene recombinant human superoxide dismutase liposome enteric-coated capsule can strengthen the absorption of the human Cu-Zn superoxide dismutase, improve the blood concentrations and pesticide effect, remove the free radical from human body better, and delay the process of aging. The encapsulation ratio of the human Cu-Zn superoxide dismutase (Cu-Zn SOD) liposome is 80.54 + / - 2.26 percent, and the tolerances of the enteric-coated capsule under the simulated condition of gastric acid, pepsin and pancreatic enzyme in vitro are respectively 95.2 percent, 91.6 percent and 86.2 percent.

The invention discloses a preparation method of insulin glargine and an analogue thereof. The preparation method includes the following steps: (1) a gene engineering method is used for preparing a precursor of the insulin glargine and the analogue of the insulin glargine with a chain B and an end C containing a plurality of basic amino acids; (2) an amino acid side chain protective agent is used for distinguishing arginine or lysine through pancreatic enzyme specificity, and the insulin glargine and the analogue of the insulin glargine are provided with protecting groups and obtained under the effect of the protective agent and pancreatic enzyme; or the specificity is used for acting on clostripain of the arginine (Arg) or endoproteinase lysine (Lys) C of the Lys directly without protection; (3) carboxypeptidase is added optionally to remove unprotected basic amino acids at the tail end of the C; and (4) the glargine and the analogue of the insulin glargine are obtained through deprotection. The preparation method is simple and convenient, high in yield, wide in application range and suitable for introduction of more than two basic amino acids.

The invention discloses a preparation method of water-soluble yellow fluorescent carbon quantum dots. The preparation method includes the steps: S1 uniformly mixing pancreatic enzymes, ethylenediamine and ultra-pure water to obtain mixed liquid, and performing hydrothermal reaction on the mixed liquid to prepare reaction liquid; S2 performing centrifugation, filtration, dialysis and evaporation to prepare the water-soluble yellow fluorescent carbon quantum dots. The mass volume ratio of the pancreatic enzymes, the ethylenediamine to the ultra-pure water is 1.5g: 1mL: 9mL. The quantum dots take pancreatic enzymes and the ethylenediamine as raw materials which are natural, free from toxicity, low in cost and easy to obtain, the preparation method is simple, rapid and efficient by a hydrothermal method, and the yield of the quantum dots is high.

The invention provides a culture apparatus used for temperature sensitive cells, a preparation method thereof and a cell cultureing method. The culture apparatus used for temperature sensitive cells comprises a polystyrene body; and the polystyrene body is grafted with a polystyrene-poly N-isopropylacrylamide block polymer. Compared with existing technologies, when the culture apparatus used for temperature sensitive cells is used for cell culturing, it is possible to obtain complete cultured cell layers and extracellular matrixes from deposition of the culture cell layers without damage by simple temperature variation, digestion by pancreatic enzyme or dissociation by chemical reagents is avoided, the death of cells is reduced, damages to the extracellular matrixes are lessened, and quality and activity of the cultured cells are improved.

The invention discloses a method for performing multiple-step treatment on sheepskin by using biological enzymes, which is characterized in that industrial pancreatic enzymes, composite enzymes TA for acid pickling and softening and composite enzymes TB for acid pickling and softening are respectively used for performing softening treatment on sheepskin in an enzyme softening procedure and an acid pickling procedure; and the treated sheepskin is processed through the conventional process, thereby obtaining the very soft and lightweight sheepskin. Thus, the quality and added values of the sheepskin and products thereof are greatly improved.

A method for extracting a small molecule peptide from a fresh animal bone is provided. The technological process of the method comprises the following steps: cleaning and breaking a fresh cattle or sheep bone, autoclaving, adding a special bone enzyme to conduct enzymolysis, deodorizing, filtering, concentrating, spraying the power to dry, and packaging a finished product. The method is characterized in that the temperature is higher than 120 DEG C, the pressure is 0.15-0.16 MPa and the fresh cattle or sheep bone is cooked for 8 h in the step of autoclaving; in the step of enzymolysis, the temperature is reduced to 54-56 DEG C after raising the temperature and sterilizing, NaOH is added to regulate the pH to 7.0, then the 0.5% special bone enzyme comprising an incision enzyme, an excision enzyme, a flavor enzyme and a pancreatic enzyme is added to conduct enzymolysis for 6 h; in the step of deodorizing, activated carbon is used; in the step of filtering, a candle filter and then a barrel filter are used; in the step of concentrating, the concentration pressure is -0.4 MPa to -0.6 MPa, and the concentration temperature is between 75 DEG C to 80 DEG C. The method has the advantages that the protein in the animal bone is hydrolyzed through bio-enzymes into all water-soluble nutritional ingredients, a product is high in the content of the small molecule peptide and the solubility, is favorable for absorbing by a human body, and is suitable for extracting the small molecule peptide of various animal bones.

Disclosed is a preparation method of Chinese herbal pieces processed by enzymes. The preparation method comprises the steps that firstly, single Chinese herbal medicines are extracted by using the enzymes according to Chinese herbal medicines of different categories, then medicine dregs are filtered, the medicine dregs are extracted by water / alcohol secondarily, seperatefiltrating taking and concentrating are conducted, and the Chinese herbal pieces of various forms or water-insoluble (dispersed) granules are processed; more than one of cellulase enzymes, xylanase, amylase and saccharifying enzymes is used; (2) more than one of protease, pancreatic enzymes, lipase and beta-dextranase in the Chinese herbal medicines of animals is used; (3) more than one of amylase, glucose oxidase, catalaseand beta-dextranase in the Chinese herbal medicines of starch is used; and (4) more than one of cellulase enzymes, pectinase, saccharifying enzymes and beta-dextranase in the Chinese herbal medicinesof fruit is used. According to the preparation method of the Chinese herbal pieces processed by the enzymes, the extraction rate of Chinese herbal ingredients extracted by the enzymes is increased by20-30% than that of an ordinary water extracting method and alcohol-water extracting method, and the preparation method has the advantages of being pollution-free and fully using limited resources.

The invention relates to an amino acid liquid fertilizer and an application method for improving saline-alkali soil and belongs to the technical field of green liquid organic fertilizers and saline-alkali soil improvement. The amino acid liquid fertilizer consists of the following raw materials in parts by weight: 1000-3000 parts of a uniform feather suspension solution, 5-30 parts of 3942 neutral protease, 5-30 parts of 1398 neutral protease, 5-30 parts of 166 actinomycete protease, 2-10 parts of pancreatic enzyme, 5-30 parts of urea, 1-10 parts of sodium hydrogen sulfite and 1-10 parts of manganese chloride. The biological organic liquid fertilizer has the advantage of changing wastes into valuable materials, has the functions of improving the physical and chemical properties of the saline-alkali soil and being low in carbon content and safe, and can be used for comprehensively providing organic and nutritional trace elements for plants.

The invention relates to an absolute quantitative method for biomass spectra of CYP450 enzyme hypotypes, and belongs to the technical field of proteomics. In the method, on the basis of liquid chromatography (LC) / mass spectrometry (MS) / MS, the absolute quantification of four CYP450 enzyme hypotypes, namely CYP1A2, CYP2B6, CYP3A4 and CYP3A5 in systems such as liver microsome and the like is realized simultaneously by a strategy that CYP450 generates specific peptide quantitative enzymes by pancreatic enzyme hydrolysis. The method mainly comprises the following steps of: preprocessing a proteinsample, preparing a standard curve, measuring the protein sample and preparing a quality control sample. The absolute quantitative method has excellent linear relations, is high in accuracy and repeatability, is sensitive, precise and reliable, and can be used for medicinal metabolism and the evaluation of the mutual effect of medicaments.

The present invention provides for pharmaceutical compositions comprising pancreatic enzyme preparations (PEPs) with viral infectivity reduced below significant levels and having high enzymatic activity. The PEPs can comprise lipases, proteases, amylases, non-enveloped viruses (e.g., porcine parvovirus (PPV), porcine circovirus type 2 (PCV-2), porcine encephalomyocarditis virus (EMCV)), and enveloped viruses (e.g., vesicular stomatitis virus (VSV), and influenza A (IFA)). The present invention also includes methods of treating pancreatic insufficiency by administering these pharmaceutical compositions and methods of making the same by treating the PEP with beta-propiolactone (BPL) to reduce viral infectivity.

The invention provides a preparation method and applications of pig spleen transferfactor. According to the preparation method, frozen-breaking, freeze thawing breaking, and centrifugal process are combined for transferfactor production, yield is guaranteed, and at the same time production temperature is reduced, and the activity of the transferfactor can be guaranteed preferably. And in addition, the prepared transferfactor contains no protein, is a small molecular peptide, possesses no pyrogen, is a widely used immunomodulator, is capable of increasing vaccine titer, and can be used for improving animal immunity and improving production performance; the activity is not influenced by pancreatic enzymes; and no toxic reaction is caused. When the pig spleen transferfactor is combined with vaccine, animal immunity can be improved, vaccine titer is increased at the same time, body immune response period is shortened, and antibody lasting time is prolonged.

Compositions of the present invention, comprising the combination of enterically coated and uncoated pancreatic enzyme-containing beads are useful for treating or preventing pancreatitis pain, and optionally disorders associated with digestive enzyme deficiencies.

The invention discloses a preparation method for mesenchymal stem cells from placental decidua basalis. The preparation method includes the following steps that after I-type collagenases are added to decidua basalis tissue blocks to digest for 18 h-20 h, pancreatic enzymes are added to continue to digest, filtration and centrifugation are then conducted, and the mesenchymal stem cells from placental decidua basalis are obtained; the obtained mesenchymal stem cells from placental decidua basalis are cultured and amplified. The I-type collagenases digest the decidua basalis tissue blocks for a long time so that a great number of mesenchymal stem cells can be obtained, the utilization rate of the decidua basalis tissue blocks is increased, cell culture time and the cell culture period are shortened, cell quality reduction and functional form change caused by repeated cell replication are avoided, and a good guarantee is provided for the clinical mesenchymal stem cells from placental decidua basalis.

The present invention relates to the technical field of Traditional Chinese medicine, in particular to a traditional Chinese medicine composition and the use thereof. The traditional Chinese medicine composition comprises the following ingredients: honeysuckle, tangerine peel, stir-baked malt, honey, and hawthorn juice. The traditional Chinese medicine composition of the present invention has significant promotions on the gastric emptying rate, intestinal motility, gastric digestive enzyme (pepsin) activity and pancreatic enzyme (trypsin, chymotrypsin, amylase and lipase) activity of rats, exhibiting that the traditional Chinese medicine composition of the present invention has significant effects of clearing heat-fire and moistening the intestine.