96 results about "Balanced salt solution" patented technology

Solutions for perioperative intraocular application by continuous irrigation during ophthalmologic procedures are provided. These solutions include multiple agents that act to inhibit inflammation, inhibit pain, effect mydriasis (dilation of the pupil), and / or decrease intraocular pressure, wherein the multiple agents are selected to target multiple molecular targets to achieve multiple differing physiologic functions, and are included in dilute concentrations in a balanced salt solution carrier.

A method of preparing an amniotic membrane extract including the steps of obtaining a healthy amniotic membrane from a pregnant mammal, such as a pig, cow, horse or human, homogenizing the membrane to obtain a homogenate solution, freezing the homogenate solution, and lyophilizing the frozen homogenate solution to dryness is disclosed. Preferably, the lyophilized homogenate is pulverized to a powder. The lyophilized homogenate is then reconstituted before use, e.g., in a liquid, such as a balanced salt solution or fresh amniotic fluid, or in another substance, such a gel, an ointment, a cream or a soap, depending on the intended use. Also disclosed is a pharmaceutical composition prepared according to the method of the invention, for prophylaxis and / or treatment of a disease or condition, especially of the eye or the skin. Exemplary pharmaceutically acceptable carriers for the composition of the invention include an ophthalmic solution for eye drops, a gel, an ointment, an emulsion, a cream, a powder and a spray.

A method of preparing an amniotic membrane extract including the steps of obtaining a healthy amniotic membrane from a pregnant mammal, such as a pig, cow, horse or human, homogenizing the membrane to obtain a homogenate solution, freezing the homogenate solution, and lyophilizing the frozen homogenate solution to dryness is disclosed. Preferably, the lyophilized homogenate is pulverized to a powder. The lyophilized homogenate is then reconstituted before use, e.g., in a liquid, such as a balanced salt solution or fresh amniotic fluid, or in another substance, such a gel, an ointment, a cream or a soap, depending on the intended use. Also disclosed is a pharmaceutical composition prepared according to the method of the invention, for prophylaxis and / or treatment of a disease or condition, especially of the eye or the skin. Exemplary pharmaceutically acceptable carriers for the composition of the invention include an ophthalmic solution for eye drops, a gel, an ointment, an emulsion, a cream, a powder and a spray.

A method for stabilizing collagenous eye tissues by nitrite and nitroalcohol treatment. The topical stiffening agent contains sodium nitrite or a nitroalcohol in a buffered balanced salt solution and can be applied to the surface of the eye on a daily basis for a prolonged period. Application of the solution results in progressive stabilization of the corneal and scleral tissues through non-enzymatic cross-linking of collagen fibers. The compounds can penetrate into the corneal stroma without the need to remove the corneal epithelium. In addition, ultraviolet light is not needed to activate the cross-linking process. The resulting stabilization of corneal and scleral tissues can prevent future alterations in corneal curvature and has utility in diseases such as keratoconus, keratectasia, progressive myopia, and glaucoma.

In a method for introducing a retinal implant to a position within a subretinal region of an eye, the following steps are performed: a) preparing a fornix-based scleral flap at a distance from the limbus; b) detaching the retina by subretinal injection of balanced salt solution, from the vitreous cavity and creating a localized bubble in the area of the scleral flap; c) performing in the upper hemisphere of the eye a peripheral retinotomy and detaching a part of the retina; d) advancing the implant into the subretinal space and placing an inner portion of said implant on the retinal pigment epithelium onto the desired position; and e) closing the sclera flap.

An irrigating tip have a flattened or oval shape more easily fits into the anterior chamber angle, thereby allowing more effective delivery of the pulses of balanced salt solution to the trabecular meshwork.

The invention discloses acellular cornea or acellular corneal stroma, a preparation method and application thereof. The method comprises the following steps of: (1) obtaining fresh animal full-thickness cornea or corneal stroma; (2) removing corneal epithelium, corneal endothelium and stroma cells, namely 1, soaking the full-thickness cornea or the corneal stroma in pure water at room temperature; 2, placing the soaked full-thickness cornea or corneal stroma into enzyme solution, digesting with oscillating, and washing with balanced salt solution with oscillating; and 3, repeating freeze-thaw processes of the full-thickness cornea or the corneal stroma for 4 to 8 times and washing with balanced salt solution with oscillating to obtain the acellular cornea or the acellular corneal stroma; (3) dehydrating; and (4) sterilizing and storing. In the method, the decellularization processing time of the cornea is short; the influence on the structure and the physiological property of the cornea is small; and the processed cornea has very low immunogenicity which is similar to the property of natural cornea. The acellular cornea or the acellular corneal stroma can be applied to artificial cornea construction of tissue engineering and also can serve as a medical material applied to corneal transplantation and refraction surgery.

Solutions for perioperative intraocular application by continuous irrigation during ophthalmologic procedures are provided. These solutions include multiple agents that act to inhibit inflammation, inhibit pain, effect mydriasis (dilation of the pupil), and / or decrease intraocular pressure, wherein the multiple agents are selected to target multiple molecular targets to achieve multiple differing physiologic functions, and are included in dilute concentrations in a balanced salt solution carrier.

The invention belongs to the field of biotechnology and relates to a method for extracting and culturing adipose-derived stem cells. The method comprises the following steps: (1) taking human adipose tissue, and repeatedly carrying out centrifugal washing with a D-Hank's balanced salt solution with the pH value of 7.2-7.4; (2) mincing the adipose tissue into small pieces of 1-2mm<3>, and carrying out digestion by adding a digestive juice which has the same volume of the selected adipose tissue; (3) standing and layering: repeatedly blowing and beating bottom cells with a D-Hank's balanced salt solution with the pH value of 7.2-7.4 and cleaning up; filtering a cleaned liquid through a screen mesh, removing undigested tissue, centrifuging and removing a supernatant to obtain stem cells; and (4) inoculating the obtained stem cells in a culture flask according to the density of 2-3*104 / cm<2>, adding a culture solution, and culturing in an incubator. The method has advantages as follows: digestion rate is fast; insoluble tissue blocks are minimized greatly; and an activity of the stem cells is not influenced.

An irrigation technique that can be used to increase the flow of fluid through the trabecular meshwork and to deliver a therapeutic agent or compound directly into the trabecular meshwork. Pulses of relatively high pressure irrigating balanced salt solution containing a therapeutic agent or compound can be directed at the trabecular meshwork. These pulses can be focused, or applied over a larger area. Such a technique may be practiced using the herein disclosed tip with commercially available surgical handpieces.

The invention relates to an icariin microemulsion which takes icariin as active ingredient; the icariin is dissolved in oil and then added with emulsifying agent, assistant emulsifier and water to prepare microemulsion with the micro emulsion grain diameter of 10-100nm, wherein, the oil can be one or more in oleic acid, ethyl oleate, isopropyl myristate or castor oil; the icariin microemulsion has the components with the mass percent: 0.66-6.02% of saturated solution of oil of icariin, 10-50% of emulsifying agent, 10-38% of assistant emulsifier and the balance water, Hank's balanced salt solution (HBSS), PBS buffer solution or normal saline. The icariin microemulsion can be kept clear, transparent, stable and uniform when being placed at room temperature for a year, thus good stability is verified evidently. The experiments of cells and animals prove that the icariin microemulsion can strengthen the icariin to penetrate cell layers, promote the medicine to be ingested and absorbed and enhance the healing effect of the medicine. The invention also discloses a preparation method of the icariin microemulsion.

An ophthalmic composition for artificial tears useful for treating dry eye symptoms or for general eye protection. The composition includes a physiologically balanced salt solution and one or more anti-oxidative ingredients which collectively confer on said ophthalmic composition a total antioxidant capacity as a FRAP value of greater than 100 μmol / l, preferably greater than 150 μmol / l. The composition also has peak UV absorption in the range of 200-350 nm. Suitable anti-oxidative ingredient includes ascorbate (ascorbic acid), urate (uric acid), cysteine, glutathione, tyrosine, lactoferrin, transferrin, albumin, caeruloplasmin and lacrimal gland peroxidase.

The invention discloses an eye drop and a preparation method thereof, relating to an eye drop. The invention provides an eye drop with short action time, high purity and relatively good effect and capability of simultaneously playing a role of corneal neovascularization, lymphangiogenesis and cornea inflammation resistance and a preparation method thereof. The eye drop contains the following components by volume percent: 0.1-5% of bovine serum, 5-15% of thickening agent, 1-5% of acid-base regulating solution, 0.5-2% of antibiotics, 5-20% of recombinant protein and the balance of balanced salt solution. The eye drop contains the balanced salt solution, the thickening agent, the bovine serum, the antibiotics, the acid-base regulating solution and the recombinant protein. The preparation method of the eye drop comprises the following steps of: adding the thickening agent, the bovine serum, the antibiotics and the recombinant protein to the balanced salt solution, regulating a pH value by using the acid-base regulating solution and osmotic pressure by using an osmotic pressure buffering agent, and removing bacteria through membrane filtration; or preparing the bovine serum to sterile micropowder, dissolving the bovine serum into the balanced salt solution, regulating the pH value, removing the bacteria through the membrane filtration, and dissolving recombined powder into the solution so as to obtain the eye drop.

The invention relates to a method for culturing fresh water nuclear pearls, which includes pearl nucleus picking, pearl nucleus inserting and pearl culture, wherein the pearl nucleus inserting is divided into two steps, including culturing cell tissue mantle or biological cell that has multiplication capacity, adhering to the pearl nucleus, then putting the pearl nucleus into the nucleus site in the mantle and the visceral sac of an anodonta and harvesting pearls from the anodonta after one to two years' culturing for the first step; inserting the pearl nucleus with a diameter of above 15 mm into the pear sac and conserving pearl for three to five years; the culturing of the cell tissue mantle or the biological cell thereof requires the preparation the balanced salt solution and the culture medium. The method is characterized in easy operation, high survival percent, large pearl diameter and thick pearl layer. Pearls of gem grade with the diameter of above 20 mm can be cultured.

The invention discloses a milrinone lactate injection and a production method thereof. Lactic acid is adopted for assisting in dissolving, and the pH value is adjusted, so that hyperchloremia probably caused by excessively high Cl<-> brought by the adoption of hydrochloric acid and the like for adjusting pH is prevented. Sodium chloride or a balanced salt solution is taken as a carrier isotonic agent. A titration method is adopted for metering strictly. The milrinone lactate injection has a reasonable and simple process flow, and the quality is controllable.

The present invention discloses a new application of pig skin which can be substituted for human skin and its preparation method. Said preparation method includes the following steps: killing health living while pig with about 50 kg, shaving hair and cleaning pigskin, skinning and chipping to obtain skin sheet, cross-linking with glutaraldehyde, cleaning with balanced salt solution phosphate, soaking it in IM physiological saline containing trypsase, vibrating with Triton-100, more washing it with balanced salt solution phosphate and storing it in refrigerator with 4 deg.C for stand-by. Said invented product has the functional characteristics of two skin substitutes of Integra artificial skin and ADM, so that it not only can be used as temporary wound surface covering material for protecting surface of wound, but also can be used as permanent substitute for dermis for repairing surface of the wound.

The present invention relates to a colored perfusion liquid exclusively for ophthalmic surgery. The colored perfusion liquid exclusively for ophthalmic surgery of the present invention includes conventional ophthalmic balanced salt solution and is characterized in that the present invention further contains 0.001 to 0.1mg / ml fluorescein sodium. The present invention firstly proposes the intraocular colored perfusion liquid and a concept of colored surgery, the present invention has the advantages that: the present invention can be filled in the anterior chamber, vitreous body, lens capsular bag and other lacunas, and can form the contrast with the intraocular transparent and semi-permeable membrane tissues, such as, cornea, lens capsule membrane, lens, vitreous body, retina and so on, therefore, the operator can identify such structures easily and the tissues which are difficult to identify become visible, therefore, the surgery becomes easy, the operation is accurate, the time is shorten, the retina, lens capsule membrane and other main structures are protected, the surgical complications are reduced, and better surgical effects are achieved accordingly. In addition, the present invention changes and overcomes the problems that the past localized staining method is time-consuming and needs to rinse, the surgical process is complicated, the operation is fairly troublesome, and the method can not carry out the differential display of the around tissues, that is the scope is too small etc.

An inexpensive, audible alarm to alert the surgeon when the level of Basic Salt Solution (BSS) falls dangerously low during a cataract surgical procedure. A digital weight gauge is suspended from the BSS pole of the phacoemulsification unit. The BSS bottle is suspended from the weight gauge. As the BSS leaves the bottle, the weight of the bottle decreases. When the digital weight gauge reaches a predefined minimum weight, an audible alarm is activated. The alarm may be provided with an on / off switch so that the alarm may be deactivated when it is not in use. The weight gauge may also have a programmable setting to adjust to the surgeon's preference for timing of notification.

The invention relates to the technical field of biological materials and clinical medicine, and provides an injection, a preparation method of the injection and application of the injection in dentalpulp regeneration. The injection comprises a composite preparation as a dispersion phase and a dispersion liquid as a continuous phase. The composite preparation comprises a drug, a microcarrier and stem cells, the drug is wrapped in the microcarrier, and the stem cells are attached to the surface of the microcarrier. The dispersion liquid is selected from at least one of a group consisting of a balanced salt solution, a serum-free culture medium and injectable hydrogel. The injection is mainly applied to regeneration of clinical functional dental pulp tissues, and regeneration of dental pulpin affected root canals is realized. By utilizing the injection, the formation of the nerve and vascularized dental pulp-like tissue in the root canal can be realized, the nutrition, defense and sensory functions of the dental pulp tissue are recovered, and the service life of the affected tooth is prolonged.

A method for stabilizing collagenous eye tissues by nitrite and nitroalcohol treatment. The topical stiffening agent contains sodium nitrite or a nitroalcohol in a buffered balanced salt solution and can be applied to the surface of the eye on a daily basis for a prolonged period. Application of the solution results in progressive stabilization of the corneal and scleral tissues through non-enzymatic cross-linking of collagen fibers. The compounds can penetrate into the corneal stroma without the need to remove the corneal epithelium. In addition, ultraviolet light is not needed to activate the cross-linking process. The resulting stabilization of corneal and scleral tissues can prevent future alterations in corneal curvature and has utility in diseases such as keratoconus, keratectasia, progressive myopia, and glaucoma.

Ophthalmic composition for the correction of presbyopia. Composition consisting of 2% Pilocarpine, characterized by being diluted to 1% with sterile Balanced Salt Solution (BSS), obtaining 1% pilocarpine (2.5 ml)+bromfenac, 1.8 mgrs of bromfenac (2.5 ml)+sterile Balanced Salt Solution (BSS) (2.5 ml), finally obtaining eye drops of 7.5 ml. It is also possible to obtain a 1.5% and 2% pilocarpine.

The invention provides a method and system for separating, culturing and amplifying human umbilical cord mesenchymal stem cells. The method comprises the following steps: step I, pretreating a sample; step II, obtaining Wharton's jelly, cutting the obtained Wharton's jelly into small tissue, and adding a filtered red blood cell lysis buffer solution of which the volume is 3.5 times that of the small tissues; step III, digesting collagenase, wherein a digestion solution is a balanced salt solution containing 1% of IV type collagenase, 0.5% of hyaluronidase, 300U / ml DNA enzyme and 2% of a serum substitute; step IV, performing primary cell isolated culture: adding a serum-free culture medium of mesenchymal stem cells to obtain a cell suspension, and adding a special primary cell culture solution for umbilical cord mesenchymal stem cells for primary culture; step V, subculturing the cells, and inoculating the collected cells according to 5000 cells / cm < 2 > by using trypsin with the mass percent concentration of 0.215%; and step VI, collecting the umbilical cord mesenchymal stem cells, and performing osteogenic differentiation, adipogenic differentiation and chondrogenic differentiation experiments.

The invention discloses a primary culture method for alveolar epithelial cells of Microhyla ornata. The primary culture method comprises the following steps: subjecting adult Microhyla ornate to surface sterilization; dissecting the adult Microhyla ornate, taking out the lung and carrying out cleaning with a HBSS balanced salt solution; adding a collagenase I solution for digestion; subjecting a digested solution to refrigeration and centrifugation and adding a complete cell culture medium; blowing and beating the fragments of the lung tissue, and then carrying out filtering by using a cell filter screen with a pore diameter of 100 [mu]m so as to obtain a cell suspension; and subjecting the cell suspension to culture at a constant temperature of 27 DEG C and replacing the previous completecell culture medium with a new complete cell culture medium every 3 to 4 d. The primary culture method of the invention can rapidly and repeatedly establish primary culture of the alveolar epithelialcells of Microhyla ornate; needed cell culture equipment is simple and has good operability; and acquired cells have obviously faster growth speed than the alveolar epithelial cells of mammals. The prepared alveolar epithelial cells of the invention significantly supplement existing alveolar epithelial cells of mammals and provide a novel cell material for research on major biological and medicalproblems such as terrestrial adaptability, lung development, pulmonary fibrosis and tuberculosis.

The invention provides a cell freezing medium which comprises the following components in percentage by volume: 1%-10% of a dimethyl sulfoxide solution, 1%-10% of a hydroxyethyl starch sodium chloride solution, 5%-20% of a human serum albumin solution, 2%-20% of a glucose solution, 5%-20% of a dextran 40 sodium chloride solution, 0.1%-1% of a non-essential amino acid solution and the balance of Hank's a balanced salt solution, and the sum of the above components is 100%. The cell freezing medium is simple in composition and convenient to prepare, mostly adopts medicinal-grade auxiliary materials, can meet the requirements of declaration of cell treatment drugs, is good in freezing effect, can stably keep the recovery rate of frozen cells to be 90% or above, can stably keep the recovery rate of the recovered cells to be 90% or above, is good in freezing effect, and provides a guarantee for patent medicines for cell treatment.

The invention belongs to the field of separation and culture of intestinal mucosal cells of animals, and particularly relates to a method for separation and primary culture of intestinal mucosal cells of fishes. The method comprises two steps of preparing materials, namely taking fish intestines out according to the conventional method, removing the fat outside the intestines, and digesting and separating the intestinal mucosal cells of fishes by any of the following two methods to obtain digestive juice containing free mucosal cells and cell mass, and centrifuging the obtained digestive juice containing free mucosal cells and cell mass to obtain the mixture of mucosal cells and cell mass which is used as inoculating material cells for primary culture; and culturing, namely performing primary culture by using skin collagens as a culture support vector, using the improved Dulbecco's modified Eagle medium (DMEM) liquid as a culture medium, and using the improved D-Hanks liquid as balanced salt solution at the culture temperature of between 26 and 28 DEG C and pH value of between 7.2 and 7.4 for more than 24 hours, namely attaching to the surface to ensure that the cells grow. The obtained intestinal mucosal cells of fishes grow well, and the structure and functional marking index system is perfect.

The invention relates to a method for detecting the difference of cell toxicity between atmospheric nano particles and industrial nano particles. The method comprises the following steps of: a, using a culture medium to prepare a cell suspension, inoculating the cell suspension in a cell culture dish and culturing the culture dish in an incubator for 24 hours; b, mixing a prepared particulate matter solution and the culture medium and preparing into a contaminated solution; using a D-hank's balanced salt solution to clean cells; carrying out contamination on the cells, and culturing the contaminated cells in the incubator for 4 hours; c, adding a mixed solution of a DCFH-DA fluorescent probe and the culture medium, sealing and wrapping with tin foil paper, and culturing the mixed solution in the incubator for 0.5 hour; d, observing the fluorescence intensity and the fluorescence distribution under an inverted fluorescence microscope, and photographing; and e, processing images by fluorescence analysis software to obtain initial data, analyzing and comparing. According to the method, the cell section preparation is fast and simple, and the raw material is easy to buy; by the cell detection method, the result is accurate, the influence factor is fewer, and multiple nano particles can be detected simultaneously; and laboratory reagents are safe and reliable and have no strong toxicity.

The invention relates to a method for culturing fresh water large-nucleus pearls, which includes pearl nucleus picking, pearl nucleus inserting and pearl culturing, wherein the pearl nucleus inserting is divided into two steps, including culturing cell tissue mantle or biological cell that has multiplication capacity, adhering to the pearl nucleus, then putting the pearl nucleus into the nucleus site in the mantle and the visceral sac of an anodonta and harvesting pearls from the anodonta after 10 to 20 months' culturing for the first step; inserting the pearl nucleus with a diameter of above 15 mm into the pearl sac and conserving pearl for 30 to 50 months; the culturing of the cell tissue mantle or the biological cell thereof requires the preparation the balanced salt solution and the culture medium. The method is characterized in easy operation, high survival percent, large pearl diameter and thick pearl layer. Pearls of gem grade with the diameter of above 20 mm can be cultured.

The invention discloses a pteria penguin granule cultivation method and a device adopted in the same. According to the method, square mantle pieces of the same specification are manufactured through a piece cutter and are smaller than 1 mm, the square mantle pieces are mixed in balanced salt solutions after being treated by mercurochrome liquid medicine, mantle piece suspension liquid is obtained, the mantle piece suspension liquid is injected into nuclear potentials of left bags and right bags of pearly shellfish, the mode of injecting the mantle piece suspension liquid is adopted, efficiency is improved, hurt to the pearly shellfish is reduced, and the survival rate is guaranteed. The piece cutter serves as the adopted device, the device is manufactured based on the 3D printing technology, is firm and durable and cannot contaminate the mantle pieces, the manufactured mantle pieces are small regular squares, and it is guaranteed that produced granules are even in size and approximately round. Therefore, the good application prospect is achieved.

Ophthalmic composition for the correction of presbyopia. Composition consisting of 2% Pilocarpine, characterized by being diluted to 1% with sterile Balanced Salt Solution (BSS), obtaining 1% pilocarpine (2.5 ml)+bromfenac, 1.8 mgrs of bromfenac (2.5 ml)+sterile Balanced Salt Solution (BSS) (2.5 ml), finally obtaining eye drops of 7.5 ml. It is also possible to obtain a 1.5% and 2% pilocarpine.

The invention provides a separation and extraction fluid suitable for pig pancreas islet and a preparation thereof. The fluid is characterized in that the oxygen load of the separation and extraction fluid is high and the problem of oxygen deficit in the separation and extraction process of pancreas islet can be solved. The separation and extraction fluid comprises the following steps of: first, taking a basal culture medium or a balanced salt solution as an aqueous phase; and adding collagenase, calcium chloride, DNA enzyme, bovine serum albumin, perfluorocarbon, an emulgator and an antibacterial agent for meeting the demand of separating and extracting pig pancreas islet. The separation and extraction fluid provided by the invention cannot release the anoxic degree in the separation and extraction process of pancreas islet and the survival rate, the function and the activity of the pancreas islet treated by the separation and extraction fluid provided by the invention are superior to those of pancreas islet treated by the conventional separation fluid with collagenase only.