153 results about "Vascularizes" patented technology

A system and method for opening a lumen in an occluded blood vessel, e.g., a coronary bypass graft, of a living being's vascular system. The system introduces an infusate liquid at a first flow rate to the occluded portion of the blood vessel, and withdraws that liquid and some blood at a second and higher flow rate. This action creates a differential flow in the occluded blood vessel portion and thereby prevent particles of occlusive material from flowing into any upstream blood vessel or downstream blood vessel in said living being's vascular system.

A system and method for opening a lumen in an occluded blood vessel, e.g., a coronary bypass graft, of a living being's vascular system. The system introduces an infusate liquid at a first flow rate to the occluded portion of the blood vessel, and withdraws that liquid and some blood at a second and higher flow rate. This action creates a differential flow in the occluded blood vessel portion and thereby prevent particles of occlusive material from flowing into any upstream blood vessel or downstream blood vessel in said living being's vascular system.

The invention discloses a bionic vascularized soft tissue with a multilayer vascular structure and a preparation method thereof. The preparation method comprises the steps of: employing a moulding process or 3D printing way to obtain a sacrificial membrane with a branched structure; coating or spraying a polymer solution containing a pore-foaming agent on the surface of the sacrificial membrane toobtain a polymer film; removing the treated sacrificial membrane of the structure and the pore-foaming agent to obtain a vascular channel-like structure with surface pores; conducting perfusion planting of endothelial cells in the channel of the vascular channel-like structure; planting smooth muscle cells or fibroblasts in the surface pores of the vascular channel-like structure; and placing thetreated vascular channel-like structure in a mold, adding an aquogel solution containing parenchymal cells, and conducting moulding so as to obtain the bionic vascularized soft tissue. The method provided by the invention can construct bionic blood vessels with a multilayer structure in artificial tissues.

This invention provides method of making and using of a porous 3 dimensional cyclic RGD peptide-modified alginate scaffold that can be loaded with different cell types and / or growth factors for implantation at sites of tissue damage to promote tissue regeneration. The cyclic RGD peptide promotes vascular formation of the host tissue, cell binding and survival of seeded cells. Scaffolds with growth factors but without cells can also be implanted to create a vascular bed in which cells are transplanted at a later time point.

The invention relates to a method for treatment of unstable coronary artery disease, which is characterised by an early revascularisation together with administration of a low molecular weight heparin. Preferably the low molecular weight heparin is administered up to revascularisation. The methods are also useful for treatment of unstable angina and not worsening chest pain and for treatment of non-Q-wave myocardial infarction (mild heart attack).

A method and materials to create complex vascularized living tissue in three dimensions from a two-dimension microfabricated mold has been developed. The method involved creating a two dimensional surface having a branching structure etched into the surface. The pattern begins with one or more large channels which serially branch into a large array of channels as small as individual capillaries, then converge to one or more large channels. The etched surface serves a template within a mold formed with the etched surface for the circulation of an individual tissue or organ. Living vascular cells are then seeded onto the mold, where they form living vascular channels based on the pattern etched in the mold. Once formed and sustained by their own matrix, the top of the mold is removed. The organ or tissue specific cells are then added to the etched surface, where they attach and proliferate to form a thin, vascularized sheet of tissue. The tissue can then be gently lifted from the mold using techniques such as fluid flow and other supporting material, as necessary. The tissue can then be systematically folded and compacted into a three-dimensional vascularized structure. This structure can then be implanted into animals or patients by directly connecting the blood vessels to flow into and out of the device. Immediate perfusion of oxygenated blood occurs, which allows survival and function of the entire living mass.

The invention provides a method for building a hollow vascularized heart based on the 3D biological printing technology and the hollow vascularized heart and relates to the technical field of tissue engineering and biology. The method includes: performing reverse modeling to build a heart three-dimensional grid model, importing the data of the heart three-dimensional grid model into a double-nozzle biological printing machine, and driving the nozzles of the printing machine to move according to a predesigned CAD digital model and selected forming parameters, wherein the nozzle 1 of the printing machine is loaded with sacrificial materials and used for printing a support framework, the nozzle 2 of the printing machine sprays biological ink to obtain building bodies, and the effective components of the biological ink comprise hydrogel, platelet-rich plasma, third-generation human umbilical vein endothelial cells and SD rat primary cardiomyocytes; crosslinking and cleaning the building bodies, and performing three-dimensional culture to form the hollow vascularized heart. The method has the advantages that the problem that a large-size hollow vascularized heart is hard in integrated printing, and the hollow vascularized heart built by the method is high in cell activity and has certain functions.

The present invention involves the use of nitrone free radical trapping agents in the treatment and prevention of gliomas. The agents may be used alone or combined with other traditional chemo- and radiotherapies and surgery, to treat or prevent glioma occurrence, recurrence, spread, growth, metastasis, or vascularization.

An isolated composition of matter is provided comprising a heterogeneous population of cells seeded on a surface of a scaffold, wherein the heterogeneous population of cells comprises at least one pancreatic islet, endothelial cells and fibroblast cells. Methods of generating same and uses thereof are also provided.

The present invention involves the use of 2,4-disulfonyl phenyl tert-butyl nitrone (2,4-ds-PBN) in the treatment and prevention of gliomas. The 2,4-ds-PBN may be used alone or combined with other traditional chemo- and radiotherapies and surgery, to treat or prevent glioma occurrence, recurrence, spread, growth, metastasis, or vascularization.

The invention provides a pancreatic-like structure and a construction method thereof. The construction method comprises the following steps: A, inducing stem cells and / or progenitor cells in vitro todifferentiate into pancreatic-like cell clusters; B, mixing the vascularized cells, the pancreatic-like cell clusters and a hydrogel material, and performing biological printing to obtain a pre-gel three-dimensional structure body; and C, culturing the pre-gel three-dimensional structure body by adopting a multi-cell culture solution and / or a bioreactor to obtain the pancreas-like structure body.The pancreatic-like structure provided by the invention is composed of vascularized cells and pancreatic-like tissue cells, and has the form, phenotypic characteristics and physiological functions ofnatural tissues. The method can be applied to the aspects of organoid construction, tissue / organ / human body chips, tissue engineering, regenerative medicine, in-vitro physiological model / pathologicalmodel / pharmacological model construction, cytobiology or drug research and the like.

The invention relates to the technical field of biological materials and clinical medicine, and provides an injection, a preparation method of the injection and application of the injection in dentalpulp regeneration. The injection comprises a composite preparation as a dispersion phase and a dispersion liquid as a continuous phase. The composite preparation comprises a drug, a microcarrier and stem cells, the drug is wrapped in the microcarrier, and the stem cells are attached to the surface of the microcarrier. The dispersion liquid is selected from at least one of a group consisting of a balanced salt solution, a serum-free culture medium and injectable hydrogel. The injection is mainly applied to regeneration of clinical functional dental pulp tissues, and regeneration of dental pulpin affected root canals is realized. By utilizing the injection, the formation of the nerve and vascularized dental pulp-like tissue in the root canal can be realized, the nutrition, defense and sensory functions of the dental pulp tissue are recovered, and the service life of the affected tooth is prolonged.

The invention relates to a bionic artificial bone scaffold and a preparation method thereof. The method comprises the following steps: preparing material solutions A and B; utilizing an electrostatic spinning method to respectively prepare the material solutions A and B into a central channel layer and a peripheral column layer of a scaffold, wherein nanometer fibers in the central channel layer are in same spiral directions and the fibers in two adjacent nanometer fiber layers in the peripheral column layer are in opposite spiral directions; micro-level finely simulating a three-dimensional microenvironment of an osteon; hierarchically adding growth factors, supplying a differential inducing environment to seed cells BMSCs and constructing the bionic artificial bone scaffold with vascularizing tissues in the central channel layer and bone tissues in the peripheral column layer. The staggered orientation of the nanometer fibers in the scaffold can greatly enhance the structural stability; effective mechanic support is supplied to a bone defect part; the mutually connected network frame thereof is beneficial to cell permeation growth and blood vessel and nerve growth and also is convenient to transfer nutrient substances and metabolic wastes; the bionic artificial bone scaffold has ideal popularization and application values in the fields of biomedical materials and tissue engineering.

The present invention relates generally to improved methods for tissue engineering including tissue transplantation, augmentation and regeneration. More particularly, the present invention provides a method for the generation of donor vascularized tissue suitable for use in tissue transplantation, augmentation and / or repair. The present invention further enables the use of a support matrix in the generation of an anatomical construct comprising the donor vascular tissue. The support matrix may be devised such that it has dimensions of a size and shape adapted to simulate those of tissue to be transplanted, augmented and / or repaired. In addition to its use in tissue repair, the methods and support matrix of the present invention may also find application as a means for delivering a desirable gene product to a subject. The method and support matrix of the present invention is conveniently be made available in the form of a kit, for use generally in the field of tissue engineering.

The invention relates to a regeneration material of artificial dermis substitution and a preparation method thereof. In the regeneration material of a dermis substitution for tissue engineering skin for loading rhGM-CSF, the regeneration material is formed by a heparinizing collagen-chitosan bracket loading the rhGM-CSF solution. The invention further discloses a preparation method of the regeneration material. The invention provides an artificial dermis substitution with good performance for curing the whole skin coloboma of trauma, burning, chronic skin ulcer, thereby being capable of remarkably quickening the vascularization process of the regeneration material of artificial dermis substitution, decreasing the infection risk, promoting the healing of the surface of a wound and reducing the hyperplasia of scar, and thus easing the pain of patients. The invention can be widely applied for the aspects of trauma, burning, surgery reshaping, and the like. The preparing method is simple, the source of the material is wide, the production efficiency is high, and the invention is suitable for industrial production.

The invention discloses a preparation method and an application of a biological scaffold material and SVF assistant adipose tissue. Under an aseptic condition, the biological scaffold material, a stromal vascular fraction (SVF) prepared by digesting purified adipose granules with a medical-graded collagenase, and the purified adipose particles are evenly mixed according to a certain proportion to prepare an adipose graft for filling. The SVF is a mixture of adipose mesenchymal stem cells and various cells, is transplanted after being mixed with autogenous adipose particles, and can be continued to be developed into a mature adipose tissue in vivo; the biological scaffold material can provide a support for transplanted adipose, allows the transplanted tissue to be prone to revascularization, and improves the survival rate of the transplanted tissue; the transplanted tissue is suitable for being injected with a 16 G or 18 G needle, facilitates clinical application, and can be used for repairing of concave deformity (such as tempora and cheek concave), beautifying packing of faces and chests, soft tissue function reconstruction and the like; and especially in the beautifying packing, the prepared transplanted adipose tissue can promote shape recovery and prevent liquefactive necrosis.

A computerized method for determining an objective tumor response to an anti-cancer therapy using one or more cross-sectional images can comprise receiving one or more cross-sectional images that comprise one or more cross-sectional slices of digital medical image data and identifying one or more target lesions within the one or more cross-sectional images. The method can also comprise analyzing at least one of the target lesions with an image-processing module configured to identify a total range of pixel intensities and restrict the total range of pixel intensities to a first restricted range that corresponds to pixel intensities representative of vascularized tumor. Further still, the method can comprise deriving a vascular tumor burden for the at least one of the one or more target lesions and determining the objective tumor response for the at least one of the one or more target lesions based on the vascular tumor burden.

The invention relates to the field of stem cell induction culture, in particular to a culture method for induced differentiation of hair follicle stem cells into vascular endothelial cells. The culture method for induced differentiation of the hair follicle stem cells into the vascular endothelial cells comprises steps as follows: (1) separation of rat hair follicle stem cells; (2) culture of the rat hair follicle stem cells; (3) purification of the rat hair follicle stem cells; (4) culture of in-vitro induced differentiation of the rat hair follicle stem cells into the vascular endothelial cells. The vascular endothelial cells for promoting early vascularization of skin after trauma are obtained with the culture method, so that repair of traumatic skin is promoted; meanwhile, seed cell sources can be provided for solving problems of vascularization of tissue engineering skin, ischemic disease treatment through cell transplantation and the like.

The invention provides a vascularized tumor microfluidic organ chip used for culture in vitro, and a preparation method for the vascularized tumor microfluidic organ chip. The chip comprises a PMMA module and a glass sheet, wherein the PMMA module is provided with three through holes which penetrate through the thickness direction of the PMMA module; the periphery of the upper surface of the glasssheet and the periphery of the lower surface of the PMMA module are bonded into a whole through a bonding layer, and the three through holes independently form a first cavity used for containing a tumor sphere, a second cavity used for storing culture solution, and a third cavity; in addition, the middle area of the lower surface of the PMMA module and the glass sheet form a hollow tissue cavity;and a static pressure difference generated by the culture solution with a liquid level height difference can accelerate a three-dimensional blood capillary network to be generated, and through co-culture of the three-dimensional blood capillary network and the tumor sphere, blood capillaries can be collected and can be constructed into three-dimensional vascularized tumor micro-tissues. The chipovercomes the defects of time consumption and high cost when the microfluidic organ chip is manufactured at present, the chip has a simple technology, and an application value is provided especially for researching in vitro vascularized tumor diseases.

The invention relates to a method for constructing regenerated nerve vascularized bones, cartilages, joints or body surface organs by a prefabricated female die bracket. Firstly, the female die bracket of a defected tissue or organ is printed in a three-dimension manner by using a computer assisted design / manufacturing technology; then, a surface of a periosteum and / or perichondrium is formed in a body through an operation, the female die bracket is placed and fixedly sutured on a germinal layer of the autologous periosteum and / or perichondrium to form a closed space; after the bones, cartilages, joints or body surface organs whose male die shape corresponds to a female die are regenerated in the space constructed by the female die and the germinal layer of the surface of the periosteum and / or perichondrium, a newly regenerated tissue is taken out, the female die bracket is removed, and the tissue is transplanted to the position of the defected tissue or organ to reconstruct. The newly generated tissue or organ prepared through autologous prefabrication has rich new vessels and nerves, does not contain any exogenous materials participating in tissue regeneration, is high in safety, accurate in shape at the same time, and quite similar to the normal tissue or organ.

The invention is directed to a method of inducing angiogenesis at a site in an individual in need thereof comprising administering locally to the site an effective amount of one or more agents that induce hypoxia induced factor 1α (HIF-1α). In another aspect, the invention is directed to a method of inducing angiogenesis at a site in an individual in need thereof comprising administering locally to the site an effective amount of one or more agents that induce hypoxia induced factor 1α (HIF-1α) and one or more lysophospholipids. In addition, the invention is directed to methods of generating prevascularized tissue, methods of generating a vascular network in a device and compositions thereof.

The invention relates to a vascularized tissue engineering bone and a preparation method thereof. The vascularized tissue engineering bone is composed of a support composed of poly-L-lysine (PLL) modified coralline hydroxyapatite (CHA) as well as a vascular endothelium dermatoblast patch and a bone formation cell patch which are sequentially wrapped at the inner layer of the support. According to the invention, an exogenous support obtained by virtue of the PLL modified CHA is beneficial to crawling ingrowth of blood vessels, and vascularization is realized finally, so that a precondition is provided for long term survival of cells in the tissue engineering bone, and organism immune rejection and inflammatory response are also reduced; adipose mesenchymal stem cells are selected for preparing a double cell patch, endothelial cells different in sources do not need to be added, cells are rich in resources and easy to separate and acquire, a trauma on a donor is minimal, ethical principles are not violated, and culture requirements are low. The vascularized tissue engineering bone provided by the invention is simple in preparation method and mild in preparation conditions, has good bone formation and vascularization properties, is consistent with in vivo application requirements, and has good application prospects as a novel vascularized tissue engineering bone.

Disclosed are improved methods and compositions for use in light-based in vivo imaging and treatment. The techniques described involve the use of low-scattering, oxygen-carrying blood substitutes in imaging and treatment methods, including OCT imaging. The invention has particular advantages in imaging within the cardiovascular system and highly vascularized or oxygen-dependent tissues.

The present invention relates to the generation of vascularized human heart tissue from human primordial Islet1-positive (ISL1+) progenitors, and more particularly the generation of vascularized human heart tissue from human primordial Islet1+ cardiovascular stem cells which are positive for markers ISL1+ / NKX2.5− / KDR−. One aspect of the invention relates to isolation of human ISL1+ primordial cells from human pluripotent cells, such as human ES cells or other human pluripotent stem cell sources, wherein the human ISL1+ primordial cells can differentiate into three different lineages; cardiomyocyte lineages, endothelial lineages and smooth muscle lineages. Another aspect relates to use and implantation of the human primordial ISL1+ progenitors into an animal model to generate human vascularized heart tissue, and more particularly, the production of an in vivo humanized model of vascular disease. One embodiment relates to the use of an in vivo humanized model of vascular disease as an assay, for example to assess drug toxicity and / or identify agents which increase and decrease coronary blood flow to the human vascularized heart tissue. Another embodiment relates to the therapeutic use of human primordial ISL1+ progenitors, for example, in one embodiment the invention provides methods for the treatment cardiovascular disorders and / or congenital heart disease in a subject comprising transplanting into subjects vascularized human heart tissue generated from human ISL1+ progenitors.

The invention discloses an experimental method for promoting tissue engineering pre-vascularization by umbilical cord mesenchymal stem cells; according to the method, vascular endothelial cells and umbilical cord mesenchymal stem cells with different proportions are co-cultured in a two-dimensional manner, and a rule of promoting vascular endothelial cell proliferation and hematopoietic differentiation by the umbilical cord mesenchymal stem cells is studied. In addition, a micron-sized network microvascular scaffold is prepared by high temperature molten monosaccharide and is dissolved in hydrogel to construct a continuous microchannel culture system of a three-dimensional hydrogel female die, the two cells are subjected to perfusion and suspension culture, an in-vitro constructed microvascular network structure and an engineered bone tissue in-vivo transplantation effect based on the structure are evaluated, and a mechanism of the two cells participating and promoting microvascular network construction is illuminated. A new solution and an experimental basis are provided for in-vitro pre-vascularization of engineered tissues and organs.

The invention relates to the technical field of medical device accessories, and in particular relates to a hemostatic compressor for the cardiology department. The hemostatic compressor for the cardiology department is simple in structure; compression treatment of the affected part of a patient is carried out by using a GelMA hydrogel dressing; the additional burden of a medical staff is reduced;wound surface vascularisation and epithelial regeneration are accelerated; the wound surface healing speed is increased; the infection rate is reduced; and the living quality of the patient is improved. The hemostatic compressor for the cardiology department comprises a sickbed, a reciprocating screw, a limiting rod and a GelMA hydrogel dressing patch; a left platform is arranged at the left end of the sickbed; the top of the left platform is fixedly connected with a first rodless cylinder; the first rodless cylinder is glidingly provided with a sliding block; the top of the sliding block is fixedly connected with a left bracket; the top of the left bracket is fixedly connected with a second rodless cylinder; the second rodless cylinder is glidingly provided with a movable block; a first cylinder is arranged at the bottom of the movable block; the output end of the first cylinder is provided with a box, which is hollow; the inner bottom of the box is fixedly connected with two sets ofsupport rods; and both the two sets of support rods are each provided with a first bearing.

The invention discloses a silicon-doped hydroxyapatite based vascularization-promoting porous scaffold material, and a preparation method and application of the porous scaffold material, and belongs to the technical field of biological materials. The silicon-doped hydroxyapatite based vascularization-promoting porous scaffold material has a molecular formula of Ca<10>(PO4)<6-x>(SiO4)<x>(OH)<2-x>,wherein x is more than 0 and less than 0.75. The stent material provided by the invention has directional gradient pores which are in communication along the radial direction, and meanwhile, the porescommunicate with each other, so that cell adhesion and regeneration are facilitated, and whole-layer skin repair related cells grow into the center from the edge and the bottom of a wound. In addition, the scaffold material prepared by the preparation method also has good biocompatibility, controllable biodegradability and mechanical property, and has a wide application prospect.

The invention discloses a method for preparing a multilayer spiral urethral tissue engineering scaffold. A thermoplastic degradable polymer is used as a printing raw material, a rectangular strip embryoid body with hollow patterns by adopting a fused deposition type 3D printer, an embryoid body spiral tube is obtained by spirally curling, and after heat treatment and nesting, a double-layer scaffold consisting of an inner-layer embryoid body spiral tube and an outer-layer embryoid body spiral tube is obtained, one end of the double-layer scaffold is sewed by a polylactic acid suture, an endothelial cell sheet layer and a urothelial cell sheet layer which are transfected with vascular endothelial growth factors and fibroblast growth factors during culture and a smooth muscle cell sheet layer which is transfected with human platelet growth factors during culture are lined on the inner wall of the outer embryoid body spiral tube in sequence, extracellular matrix is filled, the other end of the double-layer scaffold is sewed, and the urethral tissue engineering scaffold can be obtained after thrombin is fixed. The scaffold prepared by the invention can keep a urethra smooth, is beneficial to healing the injured urethra tissue, realizes the vascularization of a urethra scaffold in time and can quickly realize the revascularization.