The invention provides a pancreatic-like structure and a construction method thereof. The construction method comprises the following steps: A, inducing stem cells and / or progenitor cells in vitro todifferentiate into pancreatic-like cell clusters; B, mixing the vascularized cells, the pancreatic-like cell clusters and a hydrogel material, and performing biological printing to obtain a pre-gel three-dimensional structure body; and C, culturing the pre-gel three-dimensional structure body by adopting a multi-cell culture solution and / or a bioreactor to obtain the pancreas-like structure body.The pancreatic-like structure provided by the invention is composed of vascularized cells and pancreatic-like tissue cells, and has the form, phenotypic characteristics and physiological functions ofnatural tissues. The method can be applied to the aspects of organoid construction, tissue / organ / human body chips, tissue engineering, regenerative medicine, in-vitro physiological model / pathologicalmodel / pharmacological model construction, cytobiology or drug research and the like.