Method for constructing regenerated nerve vascularized bones, cartilages, joints or body surface organs

Abstract

The invention relates to a method for constructing regenerated nerve vascularized bones, cartilages, joints or body surface organs by a prefabricated female die bracket. Firstly, the female die bracket of a defected tissue or organ is printed in a three-dimension manner by using a computer assisted design / manufacturing technology; then, a surface of a periosteum and / or perichondrium is formed in a body through an operation, the female die bracket is placed and fixedly sutured on a germinal layer of the autologous periosteum and / or perichondrium to form a closed space; after the bones, cartilages, joints or body surface organs whose male die shape corresponds to a female die are regenerated in the space constructed by the female die and the germinal layer of the surface of the periosteum and / or perichondrium, a newly regenerated tissue is taken out, the female die bracket is removed, and the tissue is transplanted to the position of the defected tissue or organ to reconstruct. The newly generated tissue or organ prepared through autologous prefabrication has rich new vessels and nerves, does not contain any exogenous materials participating in tissue regeneration, is high in safety, accurate in shape at the same time, and quite similar to the normal tissue or organ.

Description

technical field [0001] The invention relates to the fields of medicine and bioengineering, in particular to a method for constructing regenerated neurovascularized bone, cartilage, joint or body surface organ. Background technique [0002] Tissue and organ defects and dysfunction caused by various traumas, tumors and other reasons are the main causes of human injury. After human tissues and organs are damaged, how to repair and reconstruct the structure and function of the damaged tissues and organs is a major problem faced by the fields of biology and clinical medicine. Defects of important tissues and organs usually need to be repaired by autologous or allogeneic tissue or organ transplantation, but there are many unavoidable defects in the methods of autologous and allogeneic transplantation, such as insufficient source of donor sites for autologous transplantation, immune rejection, ethical issues etc. [0003] The concept of tissue engineering was proposed in the 1980...

AI Technical Summary

Problems solved by technology

However, on the one hand, these methods still do not completely avoid the involvement of in vitro culture process, exogenous growth factors, exogenous genes, etc.

Moreover, after implantation of regenerative tissue, exogenous cells, materials, growth factors, etc. can easily cause complications such as rejection, infection, cyst formation, non-degradable materials, and exposure. This safety problem has not been completely resolved, which seriously restricts The clinical application of the above-mentioned vascularized nerve bone, trachea, articular cartilage, etc.

On the other hand, the currently constructed tissue is difficult to be neurovascularized, so it degenerates after implantation in the body, cannot further fuse with the autologous growth, etc., and still cannot meet the requirements for defect repair and functional reconstruction

Third, joints or other organs are composed of multiple tissue structures, and simply constructing a tissue form in vitro is far from meeting the requirements of the body

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