181results about How to "Reduce immune rejection" patented technology

The invention relates to a method for preparing a biomaterial of acellular small intestinal submucosa, which comprises the steps of preposition treatment, acellular treatment, enzyme treatment, preparations of membranous products and particle products. Compared with the prior art, the biomaterial has higher bioactivity and biocompatibility, no obvious immune rejection and no toxic effect on cells; besides, the biomaterial has a certain mechanical strength and toughness, and variable shape, size and thickness, thereby being convenient for clinical suturing and fixing. At the same time, the preparation method has the advantages of unlimited raw material source, cell-free residues, no ethical issues and being capable of effectively inactivating virus. The prepared products are applicable to the biomedical engineering fields, such as repairing defections of body tissue, serving as tissue filling materials, repairing facial depression deformity, serving as tissue reinforcements to replace fascia, repairing membranous defections and malnourished and infected surfaces of wound, serving as materials for biodegradable stents, and serving as injectable filling materials.

Fish derived serine proteinases including trypsins and chymotrypsin derived from cod such as Atlantic cod is used for treating and / or preventing a variety of diseases and disorders such as inflammatory diseases, infectious diseases caused by viruses, bacteria and fungal species and diseases where a receptor binding mechanism is involved in the pathogenesis. Pharmaceutical and cosmetic compositions comprising the proteinases are described.

Methods and compositions for producing therapeutic agents using chondrocytes are provided. The genetically engineered chondrocytes can be used to express the therapeutic agent in a subject, including in an environment typically associated with chondrocytes and in an environment not typically associated with chondrocytes.

The present invention provides porcine CMP-N-Acetylneuraminic-Acid Hydroxylase (CMP-Neu5Ac hydroxylase) protein, cDNA, and genomic DNA regulatory sequences. Furthermore, the present invention includes porcine animals, tissues, and organs, as well as cells and cell lines derived from such animals, tissues, and organs, which lack expression of functional CMP-Neu5Ac hydroxylase. Such animals, tissues, organs, and cells can be used in research and in medical therapy, including in xenotransplantation, and in industrial livestock farming operations.

The invention relates to anti-bioadhesion polyelectrolyte gel as well as a preparation method and application thereof. The preparation method comprises the steps of uniformly mixing one or several polyelectrolyte polymer solutions with opposite charges in a charge ratio of 1 to 1, and generating gel by virtue of a physical crosslinking method, or a chemical crosslinking method or the combination of two methods, wherein positive charges and negative charges are balanced, and the form gel is electroneutral. The preparation method of the polyelectrolyte gel is simple and easy to operate, and theprepared gel has good protein adsorption resistance, is high in water content, does not cause inflammatory reaction and is beneficial to the mass transfer between a transplant and an organism. Therefore, the polyelectrolyte gel can be widely applied to the biomedical fields, particularly can be used for treating diabetes mellitus through packaging of islet cells and can be used as a drug release carrier, a wound dressing, a tissue repair scaffold material and the like.

The present invention provides porcine isogloboside 3 (iGb3) synthase protein, cDNA, and genomic DNA regulatory sequence. The present also invention includes porcine animals, tissue and organs as well as cells and cell lines derived from such animals, tissue and organs, which lack expression of functional iGb3 synthase. Such animals, tissues, organs and cells can be used in research and in medical therapy, including xenotransplantation. In addition, methods are provided to prepare organs, tissues, and cells lacking the porcine iGb3 synthase gene for use in xenotransplantation.

An injectable decellularized fat-matrix microparticle and applications thereof in implants are disclosed. The microparticle is prepared by rinsing, disinfecting and rising fat mass, performing repeated freezing-thawing, cutting the fat mass into slices, soaking the slices, degreasing, decellularizing, performing freeze-drying, and performing low-temperature grinding. The microparticle can be cooperated with an implant auxiliary and adopted as an injectable decellularized fat-matrix microparticle implant. The microparticle has advantages of good biocompatibility, capability of promoting tissue regeneration, and the like. As the microparticle has a three-dimensional structure, the microparticle can be adopted as a support for supporting tissue and cell growth. Active components of the microparticle can induce and regulate cell adhesion, growth, proliferation and differentiation and can promote tissue repairing and regeneration. The implant can be adopted as a minimally invasive plastic injection implanting material, and the implant has a filling function and a tissue repairing function as the natural three-dimensional structure and a lot of the active components of fat are preserved.

Fish derived serine proteinases including trypsins and chymotrypsin derived from cod such as Atlantic cod is used for treating and / or preventing a variety of diseases and disorders such as inflammatory diseases, infectious diseases caused by viruses, bacteria and fungal species and diseases where a receptor binding mechanism is involved in the pathogenesis. Pharmaceutical and cosmetic compositions comprising the proteinases are described.

The invention specifically relates to a method for extracting immune cells from marrow, belonging to the technical field of cytobiology. The method comprises the following steps: (1) acquiring human marrow, diluting the human marrow with an alpha-MEM nutrient solution containing fetal calf serum, then carrying out centrifugation and discarding supernatant and a fat layer so as to obtain marrow cells; (2) transferring human peripheral blood into a centrifuge tube, carrying out centrifugation, sucking up blood plasma at an upper layer for subsequent usage and cells at a lower layer for separation of peripheral blood lymphocytes, and adding an alpha-MEM nutrient solution containing the blood plasma at the upper layer into the marrow cells to prepare a cell suspension; (3) adding a separating medium into another centrifuge tube, flatly spreading the cell suspension onto the separating medium, carrying out centrifugation, and sucking up albuginea-layer cells so as to obtain immune cells; and (4) adding isopyknic immune cells into an immune cell cryopreservation solution, carrying out uniform mixing and carrying out immune cell cryopreservation. The method provided by the invention can effectively improve the separation rate of the immune cells and the survival rate of the immune cells after cell cryopreservation and enhances the security of cells transfused back to a patient.

The invention relates to a tissue repairing material with bioactivity and a preparation method thereof. The material is made by compounding human body living cells, and extracellular matrixes and cell growth factors synthesized and excreted by the human body living cells on the acellular small intestine submucosa. Natural antigen components are removed from the prepared tissue repairing material with bioactivity, and when the tissue repairing material is applied to a wound, the tissue repairing material can survive and directly take part in the repair of the wound to continuously synthesize and excrete growth factors, guide the cells around the wound to grow in and the creation of blood vessel, and induce the differentiation of stem cells to skin cells, thereby obviously promoting the wound healing; besides, the tissue repairing material not only has part of the characteristics of human body tissues and the good mechanical property of the acellular small intestine submucosa, but also has high biocompatibility to human body, obviously reduces the immune rejection; at the same time, the prepared tissue repairing material can cover the surface of wound, fill the defection of soft tissues, promote the growth and proliferation of the cells around the wound, repair the defection of the soft tissue organs, and promote the wound healing.

The invention provides methods and compositions utilizing FasL armed donor graft cells to reduce or eliminate host allogeneic or xenogeneic graft rejection, and FasL armed host cells to reduce or eliminate graft versus host disease.

The invention relates to a method for preparing biologically active artificial cornea. It takes animal cornea basic material as raw material, promoting cornea growth or / and preventing partial lesion by enzyme slaking, repeated unfreezing, washing, and irradiating with 60Co. It employs physical and chemical method to remove cell component and soluble protein which will cause immune response, and retains external base material construction, adds multifunctional component which is favor for cornea cell growth or / and preventing partial lesion, freeze dries and stores it. The tensile strength and diopter of prepared artificial cornea are similar to that of normal cornea, it can prevent lesion and dissolution after being implanted in, and promote cornea epithelium regeneration and collagen synthesis; the biocompatibility is good, no obvious immune rejection reaction and no toxic to cell, and can be used to treat various eye injury.

The invention discloses a dura mater biopatch and a preparation method thereof. The method comprises the following steps: A) separating the small intestinal submucosa of an animal; A2) degreasing: immersing the small intestinal submucosa in ethanol having a concentration of 90-100% at normal temperature under an ultrasonic condition for 0.5-12 h; A3) performing any one or more of decellularization, DNA removal, alpha-Gal antigen removal and alkali treatment; and A4) performing freeze drying and disinfection. The dura mater biopatch is prepared through the method. The invention concretely provides the dura mater biopatch prepared from the SIS (small intestinal submucosa), having low content of an immunogen substance and being able to reserve the three-dimensional structure of a natural extracellular matrix, and the preparation method thereof.

The invention relates to a construction method of a mesenchymal stem cell bank. The construction method comprises the following steps of first, adding 0.2w / v% collagenase II of 1-3 times of volume into tissue fragments containing mesenchymal stem cells for digestion; after digestion is finished, adding an eluent, mixing uniformly and centrifuging, and taking supernate to obtain MSCs; adding the eluent into the supernate again, mixing uniformly and centrifuging, then discarding the supernate, and acquiring white MSCs precipitate; next, adding a red cell lysis buffer into the acquired MSCs precipitate for lysis; after lysis, adding the eluent, centrifuging and discarding supernate, and performing cell counting and inoculation culture on the MSCs; and then cultivating and propagating the MSCs till the quantity of the cells reaches a storage standard, and digesting and freezing to store. The method comprises the easy operation steps and is completely suitable for clinical tests, clinical application and scientific research; the cell purity is high, and the stored seed cells contain no exogenous serum.