The invention discloses a rice seedling machine seedling planting diaphragm and a manufacture method of the rice seedling machine seedling planting diaphragm. By aiming at the problems that the seedling root winding effect in the rice seedling machine seedling planting is poor, the seedlings can easily get scattered, and the operation is complicated, the invention provides the rice seedling machine seedling planting hemp-base diaphragm, which consists of a hemp fiber diaphragm substrate and rice seeds fixedly carried on the substrate. The hemp fiber raw materials are subjected to loosening, scattering and mechanical stretching to form a net, then, biodegradable bonding agents are adopted for bonding, baking is carried out, the hemp fiber diaphragm substrate is formed, and next, the rice seeds are uniformly scattered and pasted on the hemp fiber diaphragm substrate through the biodegradable bonding agents. When the rice seedling machine seedling planting hemp-base diaphragm provided by the invention is adopted, the seedling culture operation can be greatly simplified, farmers can realize the seedling culture without seeding, in addition, the seedling root system winding effect can be obviously promoted, the winding force is improved, seedlings conforming to the machine seeding requirements are formed, meanwhile, because the seedling quality is improved, the green returning speed of the seedlings after the machine seedling planting is high, the tillering effect is good, the rice yield is obviously improved, and good economic benefits are realized.