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Micro-fluidic chip culture device of cell capable of being stretched mechanically and application thereof

A microfluidic chip, cell culture technology, applied in tissue cell/virus culture devices, enzymology/microbiology devices, biochemical cleaning devices, etc. Simple process and simple production method

Active Publication Date: 2013-06-12
UNIVERSITY OF CHINESE ACADEMY OF SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, there is also a loading device that presses down the elastic polyacrylamide gel through a circular hollow pressure head, so that it produces uniform and stable equibiaxial deformation to stretch the cells, and investigates the liquefaction and solidification of smooth muscle cells by tensile strain (Chen, C.; Krishnan, R.; Zhou, E.; Ramachandran, A.; Tambe, D.; Rajendran, K.; Adam, R.M.; Deng, L.; Fredberg, J.J. PloS one 2010, 5, e12035.), but the above devices that generate mechanical tensile stress on cells are complex, difficult to operate, and difficult to promote

Method used

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  • Micro-fluidic chip culture device of cell capable of being stretched mechanically and application thereof
  • Micro-fluidic chip culture device of cell capable of being stretched mechanically and application thereof
  • Micro-fluidic chip culture device of cell capable of being stretched mechanically and application thereof

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Effect test

Embodiment 1

[0042] Example 1. Mechanically stretchable microfluidic chip cell culture device

[0043] Such as figure 1 As shown, the mechanically stretchable microfluidic chip cell culture device provided by the present invention includes a cell culture layer 1, a PDMS film 2 and a liquid flow control layer 3 stacked together in sequence; wherein, the thickness of the cell culture layer 1 is 0.5 cm, its material is PDMS, the thickness of PDMS film 2 is 100 μm, the thickness of liquid flow control layer 3 is 0.5cm, its material is PDMS; There are 4 independently connected microchannels 5 on the liquid flow control layer 3, micro The height of the channel 5 is 50 μm, and the width is 300 μm; three cylindrical chambers 6 are arranged on each microchannel 5, and the height of the chamber 6 is equal to that of the microchannel 5, and each adjacent two cavities The distance between the chambers 6 is 1 cm, and the diameter of the chambers 6 is 0.55 cm; on the cell culture layer 1, a cylindrica...

Embodiment 2

[0059] Embodiment 2, investigate the impact of mechanical tensile strain on cell viability

[0060] Taking vascular endothelial cells (EA.hy926) as an example below, the microfluidic chip device provided by the present invention is used to investigate the effect of mechanical stretching on cell activity.

[0061] First, the entire chip is sterilized. The sterilization sequence is as follows: wash 2 times with 75% alcohol, wash 3 times with sterilized PBS, put it into the ultra-clean table for 30 minutes of ultraviolet irradiation, and then wash 2 times with PBS, and finally add 20 μl of medium to each well for use.

[0062] Take the cells cultured in a conventional culture dish, observe under the microscope when the confluent growth area reaches 75%, and inoculate them into a sterilized microfluidic chip. Adjust the cell suspension concentration to 1.5╳10 before inoculation 5 cells / ml, inoculate 20 μl of the above cell suspension in each cell culture pool. When inoculating,...

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Abstract

The invention discloses a micro-fluidic chip culture device of cells capable of being stretched mechanically. The cell culture device comprises a cell culture layer, a polydimethylsiloxane (PDMS) film and a liquid flow control layer, wherein the cell culture layer, the PDMS film and the liquid flow control layer are overlaid in sequence. At least one micro channel is arranged on the liquid flow control layer, at least one chamber is arranged on the micro channel, and the height of the at least one chamber is equal to that of the at least one micro channel. Cell culture ponds are arranged on the cell culture layer, the number of the cell culture ponds is equal to that of the at least one chamber, the PDMS film is arranged at the bottom of the cell culture pond, and the shape of the cell culture pond is identical to that of the at least one chamber. Along the direction from the liquid flow control layer to the cell culture layer, the positions of the cell culture ponds and the at least one chamber correspond mutually, the inlet and the outlet of the at least one micro channel penetrate through the PDMS film and the cell culture layer in sequence to be communicated with the outside. The micro-fluidic cell culture device can be used for researching the response of the cells to mechanical stretching force, and is simple, sensitive and reliable.

Description

technical field [0001] The invention relates to a mechanically stretchable microfluidic chip cell culture device and an application thereof, belonging to the technical field of cell culture in a lab-on-a-chip system. Background technique [0002] Cell culture is an important technology in the fields of biology and health related research. With the rapid development of life sciences, cell culture has become an important basis for the research of cell biology, molecular biology, genetics and immunology. In order to deeply explore the growth and activity of cells, the pathology of related diseases and the pharmacological (toxicological) mechanism of drugs, it is of great significance to develop different targeted cell culture methods. Currently, various culture flasks (dishes) are widely used for cell culture, which is limited to a single static culture environment. However, under normal physiological conditions, cells are often in a complex mechanical environment, such as bo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00C12M1/34
Inventor 丁永胜卫元晨范蓓媛刘春彦
Owner UNIVERSITY OF CHINESE ACADEMY OF SCIENCES
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