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Method for preparing PRP (platelet-rich plasma) of umbilical cord blood

A platelet-rich plasma and umbilical cord blood technology, applied in the field of biomedicine, can solve the problems of low availability of platelet growth factor, low platelet content, and poor availability of the elderly

Inactive Publication Date: 2016-12-21
孔五一
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0013] ①The blood source is peripheral blood, suitable for young and middle-aged people, but the usability for the elderly is very poor
With the increase of age, the activity of human platelets becomes worse, and the availability of growth factors released by platelets is very low. At this time, the PRP extracted from peripheral blood may not meet the needs of treating diseases.
[0014] ② The platelet content in the PRP obtained by the prior art method is less, which will have an impact on the therapeutic effect

Method used

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  • Method for preparing PRP (platelet-rich plasma) of umbilical cord blood
  • Method for preparing PRP (platelet-rich plasma) of umbilical cord blood
  • Method for preparing PRP (platelet-rich plasma) of umbilical cord blood

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Experimental program
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Effect test

Embodiment 1

[0088] (1) Platelet concentration in whole blood before separation: 0.25×10 9 / ml, the whole blood volume is 25ml, and the total platelet count is about 6.50×10 9 .

[0089] (2) After the first centrifugation at 150g, after 20min, the platelet concentration in plasma is about 0.713×10 9 / ml, the plasma volume is 9ml, and the total platelet count is about 6.417×10 9 , concentrated 2.854 times.

[0090] (3) After the second centrifugation at 3500g for 10 minutes, 4ml of plasma was retained, and the platelet concentration was about 1.318×10 9 / ml, the total platelet count is about 5.272×10 9 , concentrated 5.273 times.

[0091] The first centrifugation, 150g, 20min, the whole blood is divided into two layers, take all the plasma part of the upper layer (1mm above the interface with the lower layer) and mix it into a conical 50ml sterile centrifuge tube. The measured platelet concentration is about 0.713 ×10 9 / ml plasma, the platelet concentration of the original whole blo...

Embodiment 2

[0093] (1) Platelet concentration in whole blood before separation: 0.25×10 9 / ml, the whole blood volume is 25ml, and the total platelet count is about 6.50×10 9

[0094] (2) After the first centrifugation at 220g, after 20min, the concentration of platelets in plasma is about 0.703×10 9 / ml, the plasma volume is 9ml, and the total platelet count is about 6.327×10 9 , concentrated 2.812 times.

[0095] (3) After the second centrifugation at 7000g for 10 minutes, 4ml of plasma was retained, and the platelet concentration was about 1.326×10 9 / ml, the total platelet count is about 5.304×10 9 , concentrated 5.304 times.

[0096] The first centrifugation, 220g, 20min, the whole blood is divided into two layers, take all the plasma part of the upper layer (1mm above the interface with the lower layer) and mix it into a conical 50ml sterile centrifuge tube. The measured platelet concentration is about 0.703 ×10 9 / ml plasma, 2.812 more concentrated than the original whole bl...

Embodiment 3

[0098] (1) Platelet concentration in whole blood before separation: 0.25×10 9 / ml, the whole blood volume is 25ml, and the total platelet count is about 6.50×10 9

[0099] (2) After the first centrifugation at 200g, after 20min, the concentration of platelets in plasma is about 0.711×10 9 / ml, the plasma volume is 9ml, and the total platelet count is about 6.399×10 9 , concentrated 2.844 times.

[0100] (3) After the second centrifugation at 5000g for 10 minutes, 4ml of plasma was retained, and the platelet concentration was about 1.322×10 9 / ml, the total platelet count is about 5.288×10 9 , concentrated 5.286 times.

[0101] The first centrifugation, 200g, 20min, the whole blood is divided into two layers, take all the plasma part of the upper layer (1mm above the interface with the lower layer) and mix it into a conical 50ml sterile centrifuge tube. The measured platelet concentration is about 0.711 ×10 9 / ml plasma, the platelet concentration of the original whole b...

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Abstract

A method for preparing PRP (platelet-rich plasma) of umbilical cord blood includes steps: subjecting fresh umbilical cord blood to low-speed centrifuging, removing a lower cell layer, and subjecting upper platelet containing plasma to ultrahigh-speed centrifuging until the liquid is separated into two layers including an upper plasma layer and a lower layer of precipitates which are platelets; removing the upper plasma layer, leaving a small quantity of plasma in an original centrifugal tube, well mixing, vibrating, counting the platelets, and well mixing according to a proportion that 1ml of plasma contains 1.2+ / -0.125 billion of platelets approximately. The platelet-rich plasma is effective in treatment of cartilage injuries, pain alleviation and function restoration and especially remarkable in treatment of senile osteoarthritis.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a method for preparing platelet-rich plasma from umbilical cord blood, the prepared plasma preparation and its application in the medical field. Background technique [0002] As China enters an aging society, the high incidence of senile osteoarthritis has brought endless suffering to human beings, and there are more women than men. The disease can occur in all joints of the whole body, but it is more likely to occur in the knee joints, hip joints, spine and finger joints with large loads. Especially in the majority of knee and hip joint lesions. [0003] With the rapid development of bioscience and technology, a large number of experiments and studies have found that local injection of platelet-rich plasma is particularly effective in the treatment of senile osteoarthritis, and many articles on the treatment of osteoarthritis with platelet-rich plasma have been published. Platelet-r...

Claims

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Application Information

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IPC IPC(8): A61K35/51A61P19/02A61P29/00A61P19/08
CPCA61K35/51
Inventor 孔五一
Owner 孔五一
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