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153results about How to "Effective inactivation" patented technology

Production method of human fibrinogen

ActiveCN101703763AEnsure product safetyLow content of impurity proteinPowder deliveryPeptide/protein ingredientsProtein contentRNA
The invention provides a production method of human fibrinogen, which is against the method that domestic conventional manufacturers extract and separate human fibrinogen from component I, and the method for extracting fibrinogen from cryoprecipitate is adopted; an S / D method and a water-bath heat treating method are adopted to carry out double-virus inactivation, thereby effectively inactivating fat enveloped virus and non-fat enveloped virus, and ensuring the security of products; the purity of extracted products reaches as high as more than 90%, the protein content of impurities is low, and the clinical side reaction of the product is small. In the production process of the human fibrinogen of the invention, glycine is utilized as a stabilizer, the freeze-drying time is prolonged about 6-8 days to ensure the temperature variation of the product to be stable in freeze-dying process, but the freeze-drying time of general manufacturers is about 3 days; after being freeze-dried for 6-8 days, the product has uniform structure, then water bath heat treatment is carried out on the product, thus the product can be evenly heated in the shortest time, thereby achieving the purposes of effectively inactivating DNA and RNA non-fat enveloped virus and simultaneously ensuring the stability of human fibrinogen.
Owner:GREEN CROSS CHINA BIOLOGICAL PRODS

Process for producing immunolobulins for intravenous administration and other immunolobulin products

InactiveCN1311797AImprove product purityEfficient removalSerum immunoglobulinsAntiviralsIntrathecalImmunoglobulin products
The presenten invention also relates to a liquid immunoglobulin G (IgG) product which has a purity of more than 95%, a content of IgG monomers and dimers of at least 95% and, a content of IgA of less than 6 mg of IgA / I. The liquid product is stable and ready for instant intravenous administration.
Owner:CSL BEHRING AG

Sludge treatment method based on wet oxidation

The invention relates to a sludge treatment method based on wet oxidation. The sludge treatment method comprises the following steps: fully mixing a sludge material and hot liquid in a physical-chemical pre-treater during a pretreatment process; after the preparation of the sludge material is completed, conveying the sludge material into an oxidation reaction process; during the oxidation reaction process, carrying out wet oxidation reaction between the sludge material and oxygen of which the temperature is 60 DEG C-80 DEG C in an oxidation reactor, wherein gas and high-temperature material are generated after the wet oxidation reaction is completed, and the gas mainly contains carbon dioxide and water vapor and is directly discharged into the air; enabling the high-temperature material to enter into a flash dehydration process, wherein the high-temperature material firstly enters a flash tank for flashing to produce gas and material, the produced gas is purified and then discharged into the air; enabling the generated material to pass through a heat exchanger, then carrying out solid-liquid separation to obtain separated solids and liquid which can be used for the second time. By the sludge treatment method, the sludge can be efficiently and reasonably treated so that the treated sludge can be recycled. The sludge treatment method can be widely applied to sludge treatment.
Owner:北京绿创生态科技有限公司

High-temperature kitchen waste degradation microbial agent and preparing and using method thereof

The invention discloses a high-temperature kitchen waste degradation microbial agent and a preparing and using method thereof. A microbial strain comprises, by weight, 15-30 parts of nuby land anoxybacillus, 10-25 parts of liquefaction thermophilic bacillus, 20-40 parts of hot nitrogen bacillus and 25-50 parts of geobacillus stearothermophilus. Compared with the prior art, the high-temperature kitchen waste degradation microbial agent has the beneficial effects that as for kitchen waste with different integrants, reasonable matching on single bacterium powder can be carried out, and single bacterial strains are cooperated for degrading the kitchen waste in a collaborative mode; the number of effective viable bacteria in the single bacterium powder in the composite microbial agent is large, and when the microbial agent is used for degrading the kitchen waste, only a small number of microbial agents is needed for sufficiently and rapidly degrading the kitchen waste; components in the microbial agent are high-temperature cultures, the optimum fermentation temperature ranges from 55 DEG C to 65 DEG C, pathogenic bacteria such as escherichia coli and staphylococcus aureus in the kitchen waste can be effectively inactivated in the fermentation process, and the biosecurity is high.
Owner:SHANDONG URBAN MINING REGENERATION RESOURCE

Protoporphyrin fluorescent carbon dot, and preparation method and application thereof

The invention relates to a protoporphyrin fluorescent carbon dot, and a preparation method and application thereof. The preparation method comprises the following concrete steps: reacting protoporphyrin with polyethyleneimine (PEI) at 170 to 220 DEG C for 7 to 10 h; and then successively carrying out cooling, dialysis and filtering so as to obtain the protoporphyrin fluorescent carbon dot. The protoporphyrin fluorescent carbon dot prepared in the invention has good water solubility and maintains the intact properties of protoporphyrin; photodynamic effect produced by a porphyrin photosensitizer can effectively inactivate yeast cells, so bactericidal effect is exerted; and the preparation method has the advantages of simple reaction steps, mild reaction conditions, small danger, low toxicity, etc.
Owner:SOUTHWEST UNIVERSITY

Preparation method of high-purity human coagulation factor IX

The invention relates to a preparation method of a high-purity human coagulation factor IX, which comprises the following steps: melting refrigerated plasma, and carrying out low-temperature centrifugation; adsorbing with a DEAE (diethylaminoethanol) Sephadex A-50 gel to remove the coagulation factor IX in the cold-glue plasma; removing impure proteins in the solution by using polyethyleneglycol; carrying out S / D virus inactivation; carrying out anion exchange column chromatography to obtain a purified coagulation factor IX solution; passing through a heparin affinity column for further chromatography to obtain a high-purity coagulation factor IX solution; carrying out ultrafiltration, dialysis and concentration, and adding arginine hydrochloride and glycinate as protective agents; filtering through a 20nm filter element to remove viruses; carrying out freeze-drying; and carrying out dry heat virus inactivation. The protein protective agents are added during the gel adsorption, column chromatography and ultrafiltration dialysis, thereby lowering the activation probability of the FIX product thrombin and enhancing the qualification rate of the product. The technique has high product yield; the FIX specific activity can reach 150 IU / mg or so which is much higher than that of the traditional product; and by performing the three-step virus inactivation, the product is safe and reliable to use.
Owner:上海洲跃生物科技有限公司

Ship exhaust and ballast water integrated treatment system

The invention discloses a ship exhaust and ballast water integrated treatment system and belongs to the field of an environmental technology. The integrated treatment system comprises an electrochemical seawater modification reactor, a ballast water treatment reactor and an exhaust gas purifying scrubbing tower and is characterized in that the exhaust gas purifying scrubbing tower comprises a micro-nano-bubble generating device, an oxidizing scrubber and an alkaline absorbing scrubber. The basic principle of the system is that the electrochemical seawater modification reactor carries out electrolysis on seawater so that two liquid flows such as a strong oxidizing liquid flow and an alkaline liquid flow having different performances are formed. The strong oxidizing liquid flow can be used to deactivate microbes in ballast water and to oxidize ship exhaust. The alkaline liquid flow can be used to neutralize and absorb oxidized ship exhaust. H2 produced by the electrochemical seawater modification reactor is used for a hydrogen fuel cell so that H2 recovery and recycle are realized and explosion damage is prevented. The system provides a novel energy supply method for a ship with limited energy. The system realizes efficient treatment on ship exhaust and ballast water, has a small size and is conducive to use in the ship.
Owner:INST OF URBAN ENVIRONMENT CHINESE ACAD OF SCI

Preparing method for pig thrombin freeze-dried powder

The invention discloses a preparing method for pig thrombin freeze-dried powder. The preparing method comprises the following steps that anticoagulant pig plasma and gel are mixed and stirred for adsorption and pass through a column for elution, and a prothrombin solution is obtained; normal saline is added to rabbit brain powder, then the prothrombin solution and CaCl2 are added for zymogen activation, a crude thrombin enzyme solution is obtained, subjected to ultrafiltration for desalination and concentrated, viral inactivation is carried out, an obtained crude enzyme solution passes through DEAE-Sepharose Fast Flow chromatographic column, elution is carried out, target peaks are collected, and pig thrombin is obtained; the pig thrombin is added with mannitol or dextran 40, filtering, sterilizing, subpackaging, freeze drying and vacuum tamponing are carried out, an aluminum plastic combined cover is rolled, and a pig thrombin freeze-dried product is obtained; the freeze-dried product is subjected to dry heat treatment at 100 DEG C for secondary virus inactivation, and the pig thrombin freeze-dried powder is obtained after packaging. The specific activity of pig thrombin in the product is not lower than 130 U/mg, the whole process is simple in step and easy to implement, the product safety is improved, and the preparing method is suitable for industrial production.
Owner:WUHAN HITECK BIOLOGICAL PHARMA

Virus inactivation method for solution of globulin

The invention provides a virus inactivation method for solution of globulin. An optimum proportion with a practical value of various parameters during virus inactivation with sodium caprylate is provided by a large number of researches on various conditions such as a temperature, a pH value, concentration of caprylate, concentration of protein and hatch time during the virus inactivation. Experiments prove that the virus inactivation method for the solution of the globulin has the advantages of simpleness, safety, rapidness, effectiveness, high protein recovery and no effect on the bioactivity of a target protein.
Owner:国药集团武汉血液制品有限公司

Novel biological artificial cornea capable of realizing cellularization through in-vivo induction as well as realizing quick transparency

InactiveCN105688282ACurvature maintenanceQuick fitEye implantsTissue regenerationDepyrogenationBiocompatibility
The invention relates to a novel biological artificial cornea capable of realizing cellularization through in-vivo induction as well as realizing quick transparency. The novel biological artificial cornea is prepared with a method in the steps as follows: 1, obtaining of a cornea raw material; 2, preparation of a cornea scaffold; 3, deep modification of the cornea scaffold; 4, deep inactivation of the cornea for removal of pyrogen; 5, irradiation sterilization of the cornea; 6, cultivation and implantation of corneal endothelial cells. Deep and light lamellar biological artificial corneas with different thicknesses and a full-thickness biological artificial cornea can be established. After being implanted, the novel biological artificial cornea can be quickly healed, becomes transparent quickly and keeps good refraction, the eyesight can be recovered and the eyeball is beautified. The obtained biological artificial cornea has good mechanical performance and biocompatibility, a full-thickness cornea graft is formed after endothelial cells are implanted on a full-thickness cornea stroma scaffold and can be rebuilt in vivo, perform in-vivo induction to promote growth of corneal limbal stem cells and growth of corneal epithelial cells and become transparent quickly on the basis, the treatment efficiency and effects are improved, and an application of lamellar keratoplasty and an application of penetrating keratoplasty are both considered.
Owner:广州宏畅生物科技有限公司

Preparation method of antibacterial and antiviral nano water-based slurry

The invention provides a preparation method of antibacterial and antiviral nano water-based slurry. The preparation method comprises the following steps: dropwise adding an inorganic zinc salt solution into a sodium hydroxide solution, controlling the temperature and pH value to prepare columnar nano ZnO powder; then, mixing columnar nano ZnO powder, a Cu2SO4 water solution, and a certain amount of NaOH, CTAB, a GO water solution and hydrazine hydrate, carrying out an ultrasonic reaction to prepare Cu2O-ZnO-GO nano composite powder, finally adding the Cu2O-ZnO-GO nano composite powder into a mixed dispersion liquid, and stirring to obtain the antibacterial and antiviral nano water-based slurry. The slurry is characterized in that columnar nano ZnO and Cu2O simulate a dragonfly wing nano bulge structure; and ZnO and Cu2O are uniformly loaded by virtue of the large surface area of GO. The formed nano composite powder can effectively inactivate bacteria such as escherichia coli, staphylococcus aureus and streptococcus pneumoniae and non-enveloped viruses such as influenza viruses and SARS, and has a wide application prospect in the fields of coatings, paints, implants, household appliances and the like.
Owner:宿迁空天新材料有限公司

Anti-bacterial household paper and making method thereof

The invention discloses an antibacterial household paper, which comprises pulp, additives and antibacterial agents. The pulp comprises 45-65 parts of unbleached hardwood pulp, 10-20 parts of bamboo pulp, 15-25 parts of grass pulp, additives Including 2-4 parts of retention and drainage aid, 2-6 parts of defoamer, 1-5 parts of resin barrier remover, 2-7 parts of APAM, 3-7 parts of cationic disperse rosin, 6-10 parts of cationic starch, 2-7 parts of PPE 6 parts, 3-9 parts of surface enhancer, 2-10 parts of modified alkyd resin, 1-5 parts of amine type or quaternary ammonium cationic surfactant, antibacterial agent including 2-6 parts of quaternary ammonium salt, 3 parts of guanidine salt ~10 parts, chitosan 7~12 parts, haloamine polymer 3~8 parts; this product has the advantages of cheap price and good bactericidal effect.
Owner:YIWU YINAN PAPER IND CO LTD

Antiseptic material and a method for the production and use thereof

The invention relates to the chemical industry, in particular, to a substance which exhibits antiseptic properties and to a method for the production and use thereof. The inventive substance can be used as a filtering material or an additive for the mixture of filtering materials, or as a component of filtering composites used for disinfecting and cleaning liquids, mainly drinking water, or gases. The inventive antiseptic material consists of a solid non-water soluble cross-linked polymer material, which contains chlorine atoms reversibly bound with nitrogen atoms and is characterised by the following general formula: M(F)l.0-5.5(Ph)0,6-1.0Cl-4.2, where M - melamine, F - bridge and terminal groups-formaldehyde producers, Ph - phosphorus in oxidation state of +5, Cl - chlorine atoms bound with nitrogen atoms, wherein the above-mentioned material is cross-linked by binding phosphorus-containing ions with melamine links of polymer chains. The inventive antiseptic material producing method involves chlorinating the solid non-water soluble cross-linked polymer material which is produced by the condensation of melamine phosphate and paraform in an aqueous solution, wherein the above-mentioned material is cross-linked by binding the phosphorus-containing ions with the melamine links ofpolymer chains directly during the production process of said product.
Owner:艾库弗拉有限责任公司

A sludge treatment method based on wet oxidation

The invention relates to a sludge treatment method based on wet oxidation. The sludge treatment method comprises the following steps: fully mixing a sludge material and hot liquid in a physical-chemical pre-treater during a pretreatment process; after the preparation of the sludge material is completed, conveying the sludge material into an oxidation reaction process; during the oxidation reaction process, carrying out wet oxidation reaction between the sludge material and oxygen of which the temperature is 60 DEG C-80 DEG C in an oxidation reactor, wherein gas and high-temperature material are generated after the wet oxidation reaction is completed, and the gas mainly contains carbon dioxide and water vapor and is directly discharged into the air; enabling the high-temperature material to enter into a flash dehydration process, wherein the high-temperature material firstly enters a flash tank for flashing to produce gas and material, the produced gas is purified and then discharged into the air; enabling the generated material to pass through a heat exchanger, then carrying out solid-liquid separation to obtain separated solids and liquid which can be used for the second time. By the sludge treatment method, the sludge can be efficiently and reasonably treated so that the treated sludge can be recycled. The sludge treatment method can be widely applied to sludge treatment.
Owner:北京绿创生态科技有限公司
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