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Method used for inactivating virus based on atmospheric pressure low temperature plasma activated solution

A low-temperature plasma and plasma technology, applied in chemistry and other fields, can solve problems such as unsatisfactory sterilization efficiency, non-normal reproduction, high dosage of drugs, etc., and achieve the effects of avoiding air pollution, easy control, and simple operation

Inactive Publication Date: 2018-02-23
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional virus inactivation methods include chemical disinfectants, such as phthalone, solid peracetic acid, formaldehyde, etc., although to a certain extent they can disinfect virus contamination, but currently in the process of use, there are The dosage is too high and unscientific, which will pollute the environment
On the other hand, physical disinfection techniques, such as steam sterilizers, instantaneous high-temperature sterilizers, ultraviolet radiation, and ozone disinfection, usually use instantaneous high-temperature or high-energy rays to change the living and breeding environment of bacteria and fungi, resulting in their inability to reproduce normally. Inactivation, but the bactericidal efficiency is not ideal
And its high cost, inconvenient to carry is also one of the major limiting factors

Method used

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  • Method used for inactivating virus based on atmospheric pressure low temperature plasma activated solution
  • Method used for inactivating virus based on atmospheric pressure low temperature plasma activated solution
  • Method used for inactivating virus based on atmospheric pressure low temperature plasma activated solution

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Method for inactivating Newcastle disease virus NDV LaSota by plasma activated water

[0029] A kind of method of plasma activated water deactivation Newcastle disease virus NDV LaSota, adopt following steps: 1, cultivate Newcastle disease virus NDV LaSota strain with SPF chicken embryo allantoic fluid, specifically, NDV LaSota strain (from Beijing Academy of Agriculture and Forestry) infected 10-day-old specific pathogen-free (SPF) chicken embryos. Centrifuge at low speed to collect the allantoic fluid. Collected viruses were stored at -20 °C until use.

[0030] 2. In the experiment of plasma activated water inactivating viruses, first prepare plasma activated water, place the atmospheric pressure low temperature plasma excitation device at 2cm below the liquid surface of 10ml deionized water, open the gas source to the atmospheric pressure low temperature plasma generator Into the working gas air, adjust the gas flow rate, the flow rate of the working gas ...

Embodiment 2

[0038] Example 2 Method of Plasma Activation of Physiological Saline (PAS(NaCl)) to Inactivate Newcastle Disease Virus NDV LaSota

[0039] A kind of method that plasma activated physiological saline (PAS (NaCl)) inactivates Newcastle disease virus NDV LaSota, adopts following steps: 1, cultivate Newcastle disease virus NDV LaSota strain with SPF chicken embryo allantoic fluid, specifically, will NDV LaSota strain (provided by Beijing Academy of Agriculture and Forestry Sciences) infected 10-day-old specific pathogen-free (SPF) chicken embryos. Centrifuge at low speed to collect the allantoic fluid. Collected virus was stored at -20°C until use.

[0040] 2. In the experiment of plasma activated saline (PAS (NaCl)) inactivating virus, first prepare PAS (NaCl), and place the atmospheric pressure low-temperature plasma excitation device in 10ml of physiological saline (0.9% sodium chloride solution) liquid At 2cm below the face, turn on the gas source and feed the working gas ni...

Embodiment 3

[0046] Embodiment 3 The method of plasma activated hydrogen peroxide solution inactivating avian influenza virus

[0047] A plasma-activated hydrogen peroxide solution (PAS(H 2 o 2 )) The method for inactivating avian influenza virus adopts the following steps: 1. Use SPF chicken embryo allantoic fluid to cultivate the avian influenza virus strain, specifically, avian influenza virus H9N2WD strain (provided by Beijing Academy of Agriculture and Forestry Sciences) is infected for 10 Day-old specific pathogen-free (SPF) chicken embryos. Centrifuge at low speed to collect the allantoic fluid. Collected virus was stored at -20°C until use.

[0048] 2. In PAS (H 2 o 2 ) In the experiment of virus inactivation, first prepare PAS (H 2 o 2 ), place the atmospheric pressure low temperature plasma excitation device at 2cm below the liquid level of 10ml hydrogen peroxide solution (mass fraction is 0.3%), open the gas source and feed the working gas argon into the atmospheric press...

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Abstract

The invention discloses a method used for inactivating virus based on an atmospheric pressure low temperature plasma activated solution. The method comprises following steps: 1, gas is introduced intoan atmospheric pressure low temperature plasma generator so as to realize generation of an atmospheric pressure low temperature plasma through excitation; 2, an atmospheric pressure low temperature plasma activated solution is subjected to activation so as to obtain a plasma activated solution; 3, and the plasma activated solution is used for treating a solution containing virus. The method is capable of killing virus effectively; no toxicity, no irritation, and no peculiar smell is caused; environment pollution caused by activation of virus with chemical reagents such as formaldehyde is avoided; and in preparation process of the atmospheric pressure low temperature plasma activated solution, energy consumption is low, operation is convenient, and production cost is reduced effectively.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for inactivating viruses based on an atmospheric pressure low-temperature plasma activation solution. Background technique [0002] Plasma is the "fourth state" of matter. It is a quasi-neutral gas composed of charged particles and neutral particles that exhibits collective behavior. It is one of the disciplines based on discharge physics, discharge chemistry and reaction engineering. interdisciplinarity. According to its system temperature, it can be divided into two categories: high-temperature plasma and low-temperature plasma. Particle temperature T>10 in high temperature plasma 8 -10 9 K, the particles have enough energy to collide with each other and reach the reaction conditions of nuclear fusion. [0003] Generally, the plasma category involved in the laboratory belongs to low-temperature plasma, which is divided into hot plasma and cold plasma. Hot plasma is ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L2/18
CPCA61L2/18
Inventor 苏霞田莹周宏专杨兵张珏方竞章振华姜北宇
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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