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Novel biological artificial cornea capable of realizing cellularization through in-vivo induction as well as realizing quick transparency

A cell-inducing and bioartificial technology, applied in prostheses, tissue regeneration, eye implants, etc., can solve the problems of poor corneal epithelial healing, long time required for transparency, and poor transparency, so as to promote rapid epithelialization and accelerate Healing Speed, Ease of Storage Effects

Inactive Publication Date: 2016-06-22
广州宏畅生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Porcine cornea has a wide range of sources and is cheap. The corneal thickness, topography and refraction are very similar to human cornea. Recently, porcine corneal decellularized matrix has come out, but its transparency after transplantation is poor, and it takes a long time to become transparent. The cornea melts and the cornea Disadvantages such as poor epithelial healing

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1——Preparation of bioartificial cornea

[0028] Step 1. Obtaining the cornea: The acquisition of the corneal stent can take the following two forms:

[0029] Method 1—Select fresh pig eyeballs, remove the cornea and process the corneal stent.

[0030]Method 2—Using collagen with good biocompatibility and degradable properties as the main material, the antigen-removed bio-collagen extracted from heterogeneous or homogeneous tissues, composite chitosan, fibronectin and elastic materials, through textile technology It forms a lamellar structure that simulates the natural lamellar layer of the cornea, and achieves a certain mechanical strength.

[0031] Step 2. Preparation of corneal stent: including preparation of lamellar cornea and preparation of full-thickness cornea.

[0032] (1) Lamellar cornea preparation

[0033] ①Pre-treatment of eyeballs: Rinse the removed eyeballs with normal saline three times quickly, then soak them in 5% sodium hypochlorite solu...

Embodiment 2

[0049] Example 2 - superficial lamellar corneal transplantation in rabbits

[0050] A new type of bioartificial cornea that induces cellularization in vivo and is rapidly transparent. The cornea is obtained according to step 1, method 1 or method 2 of Example 1. Step 2 is used to construct a lamellar corneal scaffold, and step 3 is used to modify the corneal scaffold. , through step 4 to inactivate, and step 5 to sterilize by irradiation, and then use it for superficial lamellar corneal transplantation in rabbits.

[0051] Select healthy New Zealand white rabbits, 3 months old, of any gender. 1 to 2 days before the operation, use levofloxacin eye drops, 3 times a day, 1-2 drops each time. After general anesthesia, the eyelids are opened with sutures or a lid speculum, and the sutures are fixed by the superior and inferior rectus muscles. Select the trephine according to the extent of the corneal lesion, and generally use a 7-7.5mm trephine to drill the implant bed. Genera...

Embodiment 3

[0052] Example 3——Canine corneal deep lamellar transplantation

[0053] A new type of bioartificial cornea that induces cellularization in vivo and is rapidly transparent. The cornea is obtained according to step 1, method 1 or method 2 of Example 1. Step 2 is used to construct a lamellar corneal scaffold, and step 3 is used to modify the corneal scaffold. , through step 4 for inactivation, step 5 for irradiation sterilization, and then for deep lamellar corneal transplantation in dogs.

[0054] Choose a healthy Beagle, 3 months old, of any gender. 1 to 2 days before the operation, use levofloxacin eye drops, 3 times a day, 1-2 drops each time. After general anesthesia, the eyelids were opened with sutures or a lid speculum, and the sutures were fixed by the superior and inferior rectus muscles. Select the trephine according to the extent of the corneal lesion, and generally use a 7-7.5mm trephine to drill the implant bed. Generally, a trepan drill 0.25 mm smaller than th...

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Abstract

The invention relates to a novel biological artificial cornea capable of realizing cellularization through in-vivo induction as well as realizing quick transparency. The novel biological artificial cornea is prepared with a method in the steps as follows: 1, obtaining of a cornea raw material; 2, preparation of a cornea scaffold; 3, deep modification of the cornea scaffold; 4, deep inactivation of the cornea for removal of pyrogen; 5, irradiation sterilization of the cornea; 6, cultivation and implantation of corneal endothelial cells. Deep and light lamellar biological artificial corneas with different thicknesses and a full-thickness biological artificial cornea can be established. After being implanted, the novel biological artificial cornea can be quickly healed, becomes transparent quickly and keeps good refraction, the eyesight can be recovered and the eyeball is beautified. The obtained biological artificial cornea has good mechanical performance and biocompatibility, a full-thickness cornea graft is formed after endothelial cells are implanted on a full-thickness cornea stroma scaffold and can be rebuilt in vivo, perform in-vivo induction to promote growth of corneal limbal stem cells and growth of corneal epithelial cells and become transparent quickly on the basis, the treatment efficiency and effects are improved, and an application of lamellar keratoplasty and an application of penetrating keratoplasty are both considered.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to a novel bioartificial cornea that induces cellularization in vivo and rapidly becomes transparent. Background technique [0002] According to the latest statistics from WHO, corneal blindness has become the second largest blinding disease after cataract, and there are more than 50 million corneal blind patients worldwide. There are more than 5 million patients with corneal blindness in China, and more than 100,000 new patients are added every year. Viruses, fungi, bacteria and other infections, trauma, chemical injury, burns and other causes of corneal opacity or edema that seriously affect vision, as well as corneal ulcers with a large scope and deep invasion, long-term treatment, and drug treatment failure. Corneal transplantation is the key to thorough treatment, improvement of vision, and quality of life for patients with perforation risk or encroaching central cornea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/38A61L27/36A61L27/24A61L27/20A61L27/50A61F2/14
CPCA61L27/3808A61F2/142A61L27/20A61L27/24A61L27/3604A61L27/3687A61L27/50A61L2430/16C08L89/00C08L5/08
Inventor 朱楚洪曾文周静婷谭菊
Owner 广州宏畅生物科技有限公司
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