The invention relates to the technical field of biological medicines, and discloses a bionic pre-vascularization material as well as a preparation method and application thereof. The preparation method of the pre-vascularization material comprises the steps of preparing a biological material with a vein structure, performing in-vitro cell culture and performing in-vivo transplantation. The pre-vascularization material can be applied to preparation of a wound repair material, a scaffold material, an organ reconstruction material, a tissue replacement material, a tissue filling material or a wrapping material and the like. A vein network structure of a pre-lymphatic vascularization material is similar to a lymphatic network structure of the human body; and a mesh branch structure of the leafvein of the pre-vascularization material is similar to a microvascular network structure of the human body. The bionic pre-vascularization material has the mechanical properties suitable for expectable application and can keep sufficient integrity until tissues grow inwards and heal; and the bionic pre-vascularization material has the characteristic of being easily processed and shaped into a product and the acceptable storage life, but does not cause significant inflammatory or toxic response in vivo.