Multi-mode molecular imaging monitoring method of ischemia model

Abstract

The invention relates to the technical field of multi-mode molecular imaging, in particular to a multi-mode molecular imaging monitoring method of an ischemia model. By the observation method and the verification means of stem cell tracking and angiogenesis, a biological light-emitting tomography imaging method and a micro-CT (computed tomography) vascular imaging method adopted by the invention in vivo are confirmed, and the angiogenesis situation is analyzed by the survival of stem cells, three-dimensional positioning and quantitative observation and the density change of a microvascular network. The method overcomes the defect that small animal models are killed at different time points, and the continuous long-range observation of animals can be carried out.

Description

1. Technical field: [0001] The invention relates to the technical field of multimodal molecular imaging, in particular to a multimodal analysis of ischemia models. [0002] Sub-imaging monitoring method. 2. Background technology: [0003] In recent years, small animal molecular imaging technology based on ischemic type has received extensive attention, and studies on stem cell-mediated ischemic injury repair and angiogenesis in this animal model have become commonplace. However, currently, methods for monitoring the intervention effect of animal models mainly include in vitro immunohistochemistry, immunofluorescence and other means. For the survival of stem cells in small animals, it is necessary to transplant stem cells derived from transgenes with green fluorescent protein (GFP). From animal stem cells to injured animal models, the survival of stem cells needs to be sacrificed and obtained from tissues to observe the green fluorescent protein under a microscope for identi...

AI Technical Summary

Problems solved by technology

However, currently, methods for monitoring the intervention effect of animal models mainly include in vitro immunohistochemistry, immunofluorescence and other means. For the survival of stem cells in small animals, it is necessary to transplant stem cells derived from transgenes with green fluorescent protein (GFP). From animal stem cells to injured animal models, the survival of stem cells needs to be sacrificed and obtained from tissues to observe the green fluorescent protein under a microscope for identification (Katstural Jetal, Circulation Research, 2005). In vivo observation of stem cells has also been reported using bioluminescent imaging ( Bioluminescence imaging, BLI) technique to observe stem cells expressing luciferase (Cao, F. et al. In vivo visualization of embryonic stem cell survival, proliferation, and migration after cardiac delivery. Circulation 113, 1005-1014, 2006); bioluminescence imaging technology can only obtain two-dimensional plane images, and cannot intuitively display the three-dimensional space position information of the light source in the small animal body, which has great limitations in the visualization effect; in terms of quantitative analysis, bioluminescence Imaging can only be used for relative quantitative analysis, and does not have absolute quantitative significance. Please refer to: R. Weissleder and M. J. Pittet, "Imaging in the era of molecular oncology," Nature 452, 580-589 (2008). In addition, for small The animal ischemia model also needs to observe the angiogenesis after stem cell intervention. The traditional method is to take the tissue of the ischemic area to stain the vascular endothelial marker molecule CD31 with antibody, and compare the angiogenesis between the control group and the intervention group.

This method needs to kill the animal model at different stages, which brings great inconvenience to the continuous observation of the experiment

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