Multimodal molecular imaging monitoring method for ischemia model

Abstract

The invention relates to the technical field of multimodal molecular imaging, in particular to a multimodal molecular imaging monitoring method for an ischemia model. Through stem cell tracing, angiogenesis observation methods and verification means, it can be confirmed that the bioluminescence tomography method and micro-CT angiography method adopted in the present invention in vivo can be verified through the survival of stem cells, three-dimensional positioning quantitative observation and microvascular network The changes in the density of the blood vessels are used to analyze the situation of angiogenesis. This method avoids the disadvantage of sacrificing small animal models at different time points, and can observe animals continuously for a long time.

Description

1. Technical field: [0001] The invention relates to the technical field of multimodal molecular imaging, in particular to a multimodal molecular imaging monitoring method for an ischemia model. 2. Background technology: [0002] In recent years, small animal molecular imaging technology based on ischemic type has received extensive attention, and studies on stem cell-mediated ischemic injury repair and angiogenesis in this animal model have become commonplace. However, currently, methods for monitoring the intervention effect of animal models mainly include in vitro immunohistochemistry, immunofluorescence and other means. For the survival of stem cells in small animals, it is necessary to transplant stem cells derived from transgenes with green fluorescent protein (GFP). From animal stem cells to injured animal models, the survival of stem cells needs to be sacrificed and obtained from tissues to observe green fluorescent protein under a microscope for identification (Katst...

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Problems solved by technology

However, currently, methods for monitoring the intervention effect of animal models mainly include in vitro immunohistochemistry, immunofluorescence and other means. For the survival of stem cells in small animals, it is necessary to transplant stem cells derived from transgenes with green fluorescent protein (GFP). From animal stem cells to injured animal models, the survival of stem cells needs to be sacrificed and obtained from tissues to observe green fluorescent protein under a microscope for identification (Katstural Jetal, CirculationResearch, 2005). imaging, BLI) technology to observe stem cells expressing luciferase (Cao, F. et al. In vivo visualization of embryonic stem cell survival, proliferation, and migration after cardiac delivery. Circulation113, 1005-1014, 2006); biological Luminescence imaging technology can only obtain two-dimensional plane images, and cannot intuitively display the three-dimensional space position information of the light source in the small animal body, which has great limitations in the visualization effect; in terms of quantitative analysis, bioluminescence imaging can only be used Relative quantitative analysis does not have the meaning of absolute quantification, can refer to: R.Weissleder and M.J.Pittet, "Imaging in the era of molecular oncology," Nature 452,580-589 (2008). In addition, to small animal ischemia model also needs to observe For the angiogenesis after stem cell intervention, the traditional method is to take the tissue of the ischemic area to stain the marker molecule CD31 of the vascular endothelium with an antibody, and compare the angiogenesis between the control group and the intervention group

This method needs to kill the animal model at different stages, which brings great inconvenience to the continuous observation of the experiment

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