Vascularized tissue engineering bone and preparation method thereof

Abstract

The invention relates to a vascularized tissue engineering bone and a preparation method thereof. The vascularized tissue engineering bone is composed of a support composed of poly-L-lysine (PLL) modified coralline hydroxyapatite (CHA) as well as a vascular endothelium dermatoblast patch and a bone formation cell patch which are sequentially wrapped at the inner layer of the support. According to the invention, an exogenous support obtained by virtue of the PLL modified CHA is beneficial to crawling ingrowth of blood vessels, and vascularization is realized finally, so that a precondition is provided for long term survival of cells in the tissue engineering bone, and organism immune rejection and inflammatory response are also reduced; adipose mesenchymal stem cells are selected for preparing a double cell patch, endothelial cells different in sources do not need to be added, cells are rich in resources and easy to separate and acquire, a trauma on a donor is minimal, ethical principles are not violated, and culture requirements are low. The vascularized tissue engineering bone provided by the invention is simple in preparation method and mild in preparation conditions, has good bone formation and vascularization properties, is consistent with in vivo application requirements, and has good application prospects as a novel vascularized tissue engineering bone.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a vascularized tissue engineered bone constructed by an osteoblast membrane sheet and an angioblast endothelial cell membrane and a preparation method thereof. Background technique [0002] The treatment of large bone defects caused by severe trauma, tumor resection, infection, and congenital deformities is a difficult problem and a huge challenge for modern medicine. At present, the commonly used repair methods in clinic include autologous bone graft, allogeneic bone graft and the use of artificial bone, etc., but the above methods all have certain defects. Autologous bone grafting is recognized as the gold standard for bone tissue repair, but patients have to endure the trauma of autologous tissue transplantation surgery, and the donor area is limited. Therefore, autologous bone grafting cannot be regarded as an ideal repair method for large-area bone defects; allogeneic bo...

AI Technical Summary

Problems solved by technology

However, the disadvantage of the traditional "cell-scaffold" strategy is that when the seed cells are planted on the scaffold, 30-40% of the cells cannot adhere to the scaffold and are lost, and the cell adhesion rate is low and the utilization rate is poor; There are few interactions between cells, and it is difficult to form the proper microenvironment for cells in the body. After the cell-scaffold complex is implanted in the body, bone formation always occurs at the periphery of the scaffold, which affects the size and quality of osteogenesis.

However, this material has disadvantages such as not easy to shape, insufficient strength, high brittleness, and low degradation rate.

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