The invention relates to an anti-fatigue health-preservation preparation which is prepared from the following raw medicines in parts by weight: 1-15 parts of red ginseng, 10-50 parts of maca, 10-50 parts of astragalus membranaceus, 5-30 parts of angelica sinensis, 1-15 parts of pericarpium citri reticulatae and 1-15 parts of a green tea extract (theanine). The preparation is based on treatment rules of the traditional Chinese medicine theory and the modern medicine theory on a chronic fatigue syndrome, symptoms of the chronic fatigue syndrome relate to limb weakness, hyposexuality, arthralgia, head carbuncle, amnesia, depression, difficulty in thinking and the like and belong to sub-health status, and the symptoms are named as 'sluggish', 'laziness', 'fatigue of limbs', 'impotence of limbs', 'weakness of limbs' and the like in the traditional Chinese medical science. In the preparation, the red ginseng and the astragalus membranaceus have the effects of replenishing qi, strengthening spleen and nourishing blood; the angelica sinensis takes effects in heart, liver and spleen channels and has the effects of nourishing blood and promoting blood circulation; the pericarpium citri reticulatae has the effects of regulating the flow of qi, strengthening the spleen and removing dampness to reduce phlegm; the maca and the green tea extract (theanine) have direct effects of resisting fatigue and depression, relaxing nerves, resisting insomnia and enhancing memory. The six medicines are combined for use and together play the functions of strengthening the spleen, tranquilizing mind by nourishing the heart, regulating the flow of qi, soothing the livers, nourishing blood, regulating internal secretion of the body, exciting nerves and promoting automatic cooperation and mutual use of all body fluid systems, thus the fatigue and relevant symptoms of the chronic fatigue syndrome are relieved.