Method for building hollow vascularized heart based on 3D biological printing technology and hollow vascularized heart
A bioprinting, hollow-shaped technology, applied in biochemical equipment and methods, microorganisms, 3D culture, etc., can solve the problems of large-scale printing difficulties, simple hydrogels are easy to collapse, etc.
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[0032] The preparation method of the bio-ink is as follows: After mixing the third-generation human umbilical vein endothelial cells and SD rat primary cardiomyocytes with platelet-rich plasma, and then mixing with hydrogel, it is obtained. Due to the relatively fluidity of platelet-rich plasma It is better in hydrogel, so by mixing with platelet-rich plasma first, and then mixing with hydrogel, the third-generation human umbilical vein endothelial cells and SD rat primary cardiomyocytes can be evenly dispersed in the bio-ink , no cell aggregation phenomenon occurs, and the quality of bio-ink is improved.
[0033] Preferably, the content of the alginate provided in the preferred embodiment of the present invention in the bio-ink is 2-3wt%, preferably, the content of agarose in the bio-ink is 0.8-1.3wt%, preferably, the third Generation of human umbilical vein endothelial cells at a density of 1 × 10 in bioink 6 cells / ml, preferably, the density of SD rat primary cardiomyocyte...
Embodiment 1
[0055] A hollow vascularized heart prepared by a method for constructing a hollow vascularized heart based on 3D bioprinting technology, which is prepared by the following method:
[0056] (1) Cardiac modeling: Import the DICOM format of thin-slice CT scan data of volunteers into Mimics 10.0 software for 3D reconstruction, and then redesign it through reverse modeling technology to design it as a 3D mesh that can be printed with dual nozzles Model, generate STL format data, and then import it into the bioprinting control software, set relevant parameters and slice to form G code.
[0057] (2) Preparation of hydrogel: Weigh 2.5g of biological grade alginate and dissolve it in 50ml of PBS solution, stir magnetically at room temperature until completely dissolved, and autoclave it overnight in a 37°C incubator; take 1g of biological grade low melting point agarose The powder was dissolved in 50ml of PBS solution, heated until completely dissolved, and then autoclaved in a 37°C in...
Embodiment 2
[0064] A hollow vascularized heart prepared by a method for constructing a hollow vascularized heart based on 3D bioprinting technology, which is prepared by the following method:
[0065] (1) Cardiac modeling: Import thin-slice CT scan data of the volunteer heart into Mimics 10.0 software in DICOM format for 3D reconstruction, and then redesign it through reverse modeling technology to design it as a 3D mesh that can be printed with dual nozzles Model, generate STL format data, and then import it into the bioprinting control software, set relevant parameters and slice to form G code.
[0066] (2) Preparation of hydrogel: Weigh 2g of biological grade alginate and dissolve it in 50ml of PBS solution, stir magnetically at room temperature until completely dissolved, and autoclave it overnight in a 37°C incubator; take 2g of biological grade low melting point agarose powder Dissolve in 50ml PBS solution, heat until completely dissolved, autoclave and incubator at 37°C overnight. ...
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