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Method for constructing hollow vascularized heart based on 3D bioprinting technology and hollow vascularized heart

A bioprinting, hollow-shaped technology, applied in biochemical equipment and methods, microorganisms, 3D culture, etc., can solve the problems of large-scale printing difficulties, simple hydrogels are easy to collapse, etc.

Active Publication Date: 2020-12-04
叶川 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to provide a method for constructing a hollow vascularized heart based on 3D bioprinting technology. By combining 3D bioprinting technology with reverse modeling technology, the problem that simple hydrogels are easy to collapse is effectively solved, so it solves the problem of large size, Difficulty in integrated printing of hollow vascularization

Method used

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  • Method for constructing hollow vascularized heart based on 3D bioprinting technology and hollow vascularized heart
  • Method for constructing hollow vascularized heart based on 3D bioprinting technology and hollow vascularized heart

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preparation example Construction

[0032] The preparation method of the bio-ink is as follows: After mixing the third-generation human umbilical vein endothelial cells and SD rat primary cardiomyocytes with platelet-rich plasma, and then mixing with hydrogel, it is obtained. Due to the relatively fluidity of platelet-rich plasma It is better in hydrogel, so by mixing with platelet-rich plasma first, and then mixing with hydrogel, the third-generation human umbilical vein endothelial cells and SD rat primary cardiomyocytes can be evenly dispersed in the bio-ink , no cell aggregation phenomenon occurs, and the quality of bio-ink is improved.

[0033] Preferably, the content of the alginate provided in the preferred embodiment of the present invention in the bio-ink is 2-3wt%, preferably, the content of agarose in the bio-ink is 0.8-1.3wt%, preferably, the third Generation of human umbilical vein endothelial cells at a density of 1 × 10 in bioink 6 cells / ml, preferably, the density of SD rat primary cardiomyocyte...

Embodiment 1

[0055] A hollow vascularized heart prepared by a method for constructing a hollow vascularized heart based on 3D bioprinting technology, which is prepared by the following method:

[0056] (1) Cardiac modeling: Import the DICOM format of thin-slice CT scan data of volunteers into Mimics 10.0 software for 3D reconstruction, and then redesign it through reverse modeling technology to design it as a 3D mesh that can be printed with dual nozzles Model, generate STL format data, and then import it into the bioprinting control software, set relevant parameters and slice to form G code.

[0057] (2) Preparation of hydrogel: Weigh 2.5g of biological grade alginate and dissolve it in 50ml of PBS solution, stir magnetically at room temperature until completely dissolved, and autoclave it overnight in a 37°C incubator; take 1g of biological grade low melting point agarose The powder was dissolved in 50ml of PBS solution, heated until completely dissolved, and then autoclaved in a 37°C in...

Embodiment 2

[0064] A hollow vascularized heart prepared by a method for constructing a hollow vascularized heart based on 3D bioprinting technology, which is prepared by the following method:

[0065] (1) Cardiac modeling: Import thin-slice CT scan data of the volunteer heart into Mimics 10.0 software in DICOM format for 3D reconstruction, and then redesign it through reverse modeling technology to design it as a 3D mesh that can be printed with dual nozzles Model, generate STL format data, and then import it into the bioprinting control software, set relevant parameters and slice to form G code.

[0066] (2) Preparation of hydrogel: Weigh 2g of biological grade alginate and dissolve it in 50ml of PBS solution, stir magnetically at room temperature until completely dissolved, and autoclave it overnight in a 37°C incubator; take 2g of biological grade low melting point agarose powder Dissolve in 50ml PBS solution, heat until completely dissolved, autoclave and incubator at 37°C overnight. ...

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Abstract

The invention provides a method for building a hollow vascularized heart based on the 3D biological printing technology and the hollow vascularized heart and relates to the technical field of tissue engineering and biology. The method includes: performing reverse modeling to build a heart three-dimensional grid model, importing the data of the heart three-dimensional grid model into a double-nozzle biological printing machine, and driving the nozzles of the printing machine to move according to a predesigned CAD digital model and selected forming parameters, wherein the nozzle 1 of the printing machine is loaded with sacrificial materials and used for printing a support framework, the nozzle 2 of the printing machine sprays biological ink to obtain building bodies, and the effective components of the biological ink comprise hydrogel, platelet-rich plasma, third-generation human umbilical vein endothelial cells and SD rat primary cardiomyocytes; crosslinking and cleaning the building bodies, and performing three-dimensional culture to form the hollow vascularized heart. The method has the advantages that the problem that a large-size hollow vascularized heart is hard in integrated printing, and the hollow vascularized heart built by the method is high in cell activity and has certain functions.

Description

technical field [0001] The invention relates to the field of biotechnology, and in particular to a method for constructing a hollow vascularized heart based on 3D bioprinting technology and the hollow vascularized heart. Background technique [0002] Cardiovascular disease has high morbidity and mortality, which seriously threatens human health and quality of life, and also causes a huge social and economic burden. Since mature cardiomyocytes are permanent cells in the terminal differentiation stage and do not have the ability to regenerate, once necrotic, inflammatory cells infiltrate the infarcted area, degrade the cell matrix, and pathological reconstruction of the chamber wall forms fibrotic scar tissue, making the infarcted chamber wall Thinning, weakening of myocardial systolic function, further leading to ventricular enlargement, eventually developing into heart failure and even death. Although existing treatment methods can improve symptoms to a certain extent, they...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/077
CPCC12N5/0657C12N2501/90C12N2502/28C12N2513/00C12N2533/30
Inventor 叶川邹强马敏先杨龙孙宇李轩泽
Owner 叶川
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