202 results about "Platelets blood" patented technology

Methods and kits are disclosed to promote vascularization of tissue in mammals using an autologous platelet-rich plasma composition. The kit includes components for the preparation of platelet-rich plasma from the patient's blood and components for administration to an area in need of vascularization. The methods and kits are useful for the treatment of tendinosis, and in particular, lateral epicondylitis and plantar fasciitis.

The invention relates to a method and to a device for examining the properties of the global, in particular the primary, hemostasis functions in whole blood or platelet-rich plasma, comprising a storage chamber (15) for the blood to be examined, a reaction device (39) having at least one flow path (5, 16) via which the blood to be examined is transported for carrying out certain reactions, a conveyor device (1) which conveys a volumetric flow for the transport of the blood through the reaction device (39), thereby generating a conveyor pressure, a pressure gauge (8, 9) which in a blood-free pressure gauge chamber (3) measures changes in the conveyor pressure which appear depending on the reactions of the blood to be examined which have taken place in the reaction device (39), a blood collection chamber (10) for collecting the blood transported through the reaction device (39), the conveyor pressure in a pressure-sealed working chamber (12) being generated by a piston-cylinder system (1), a working surface (33) of the piston (4) forming a boundary of the working chamber (12), and the working chamber (12) being formed by the blood collection chamber (10) and the blood-free pressure gauge chamber (3). According to the method of the invention, the volumetric flow is preferably adjusted, depending on the measured pressure of the volumetric flow, in such a way that the shear rate or the shear force, whose action in the reaction opening causes blood components, in particular thrombocytes, to deposit, follows a predetermined characteristic curve and in particular is held constant.

The invention provides a cell preparation for treating osteoarthritis and a preparation method thereof. The cell preparation mainly comprises mesenchymal stem cells, differentiation-induced stem cells, platelet-rich plasma and dispersion media thereof. Compared with the prior art, the cell preparation for treating osteoarthritis has the advantages that the mesenchymal stem cells are mixed with the differentiation-induced stem cells and compounded with the platelet-rich plasma and can be dispersed in the joint cavity, so that the immune regulation function of the mesenchymal stem cells can be exerted, the microenvironment of the whole joint cavity can be improved, and inflammation remission and injury repair are facilitated; tissues can be repaired after the differentiated mesenchymal stem cells are implanted into the body, so that the repair process is shortened.

The present invention discloses one kind of injected bone repairing material and its preparation and application. The injected bone repairing material consists of solution I and solution II, the solution I is the mixture of platelet rich plasma (PRP) and 80-100 mg / ml concentration fibrinogen solution in the ratio of 1 to 3-6; and the solution II is solution mixture of calcium chloride in 40 mmol / ml concentration and thrombin in 400-1000 U / ml concentration. When the injected bone repairing material is used, solution I and solution II in the volume ratio of 1-9 are sucked with two syringes and injected into body to form gel with high adhesive force, with PRP accounting for 10-30 vol%. The injected bone repairing material is used in preparing implant for treating bone defect and bone nonunion and promoting fracture healing.

The present invention relates generally to methods and compositions for targeting and delivering solid-phase platelet-dependent vascular occlusion agents. In particular, particles or coils or stents coated with platelet binding agents are directed to target vasculature, such as the vasculature of solid tumor masses or AV-malformations or aneurysms or endoleaks; the solid-phase agent then binds and activates platelets, which in turn bind and activate other platelets. This process results in the rapid formation of a platelet-mediated thrombus about the solid-phase agent causing vessel occlusion.

The invention relates to an extraction method of platelet rich plasma (PRP) and extracted PRP and particularly relates to a method for extracting PRP from blood. The method comprises the following steps of: (a) putting the collected whole blood in a container containing an anticoagulant, and fully and uniformly mixing the blood and the anticoagulant; (b) putting the blood mixed with the anticoagulant in a centrifuge tube, carrying out primary centrifugation, and enabling the blood to be divided into three layers; (c) extracting the uppermost layer and most of the middle layer, transferring the uppermost layer and most of the middle layer into a new centrifuge tube, mixing uniformly and carrying out secondary centrifugation; and (d) discarding the plasma at the upper layer of the centrifuge tube, and resuspending the precipitated plasma by utilizing the remaining plasma, thus obtaining the PRP. The method provided by the invention has the advantages that the operation process is simple, the yield is high and the concentration of platelet in rich plasma is high.

An integrated kit for separating blood and concentrating platelet rich plasma includes (a) a main body includes an upper storage portion, a lower storage portion, and a passage portion for connecting the upper storage portion and the lower storage portion; (b) a upper cover coupled to an upper end of the upper storage portion; (c) an inner stopper inserted into a lower end of the lower storage portion so as to seal the lower storage portion; (d) a closing adjustment screw which can move vertically while penetrating a hole formed at the inner stopper and seals the passage portion; and (d) a lower cover coupled to an outside of the lower end of the lower storage portion and the outside of the inner stopper to prevent the separation of the closing adjustment screw coupled to the inner stopper in case of centrifugation.

It is intended to provide a composition for regeneration of periodontal soft tissue less invasively and a method for producing the composition. The composition for regeneration of periodontal soft tissue is prepared so as to contain a cell selected from an undifferentiated / stem cell or a blast cell having an ability to form gingiva, a matrix material and blood platelet plasma. With the composition, free gingiva as well as attached gingiva can be regenerated less invasively with an effective and good aesthetics.

The invention relates to a simple and effective method for automatically separating immune cells and extracting PRP from adult peripheral blood, in particular to a method for automatically separating immune cells and extracting platelet rich plasma (PRP) from the peripheral blood y using an AXP full-automatic cell separation device, a cryopreservation and resuscitation method used together with the method, and a method for amplifying the post-resuscitation immune cells. The method for automatically separating the immune cells and extracting PRP from the adult peripheral blood comprises the following steps: preprocessing whole-blood samples, automatically separating, collecting mononuclear cells, and optionally detecting one and more of the following projects on the obtained immune cells: the total number of the mononuclear cells, the living rate of the mononuclear cells, cell pollution, genetic diseases and HLA-ABC / DR matching. By virtue of the adoption of the special operation steps of extraction, cryopreservation, resuscitation and amplification, the immune cells can be effectively obtained and can be used for treating tumor; meanwhile, by virtue of the method, the platelet rich plasma can be effectively obtained, so that the effective clinical treatment methods can be provided.

The invention provides preparation of human platelet-rich plasma and application of the same in isolation and culture of human mesenchymal stem cells. According to the invention, erythrocytes and a mixture of leucocytes and platelets are separated from human whole blood through centrifugation, then centrifugation with a centrifugal force greater than a centrifugal force used in above-mentioned centrifugation is carried out on residual blood plasma, obtained sediment is collected and is subjected to freezing-thawing treatment respectively at a temperature no more than -20 DEG C and a temperature no less than normal temperature, and the sediment is removed through separation so as to obtain human platelet-rich plasma. The invention has the following beneficial effects: raw materials used inthe method are widely available; the blood resource is fully utilized; the obtained product of platelets is purer, contains more abundant nutritional components including growth factors and has a more ideal effect when used for isolation and culture of human mesenchymal stem cells.

The invention provides a method for building a hollow vascularized heart based on the 3D biological printing technology and the hollow vascularized heart and relates to the technical field of tissue engineering and biology. The method includes: performing reverse modeling to build a heart three-dimensional grid model, importing the data of the heart three-dimensional grid model into a double-nozzle biological printing machine, and driving the nozzles of the printing machine to move according to a predesigned CAD digital model and selected forming parameters, wherein the nozzle 1 of the printing machine is loaded with sacrificial materials and used for printing a support framework, the nozzle 2 of the printing machine sprays biological ink to obtain building bodies, and the effective components of the biological ink comprise hydrogel, platelet-rich plasma, third-generation human umbilical vein endothelial cells and SD rat primary cardiomyocytes; crosslinking and cleaning the building bodies, and performing three-dimensional culture to form the hollow vascularized heart. The method has the advantages that the problem that a large-size hollow vascularized heart is hard in integrated printing, and the hollow vascularized heart built by the method is high in cell activity and has certain functions.

The invention belongs to the technical field of preparation of platelet rich plasma and in particular discloses a method for preparing PRP (Platelet Rich Plasma) through a one-step centrifugation method. According to the method, only a high-speed automatic centrifuge is utilized, the one-step centrifugation method is adopted, only one-step liquor relief (which is used for absorbing plasma on a boundary layer) is required in the whole process, and the purposes of convenience, rapidness and low pollution risk are achieved. Moreover, according to the platelet rich plasma prepared by the method, a mean value of platelet cell concentrations reaches 1029.875*10<9> / L, the mean corpuscular hemoglobin in the PRP is 20.26 g / L, and the clinical requirements can be well met.

The invention provides a preparation method for a novel biomimetic matrix type biological wound healing material. According to the method, small intestinal submucosa with a three-dimensional scaffold structure is compounded with platelet-rich blood plasma containing a specific proportion of bioactive factors, and a specific process is adopted to carry out modification to form the novel material containing biological induction factors, wherein the novel material has a three-dimensional biomimetic structure. With the biomimetic matrix type biological wound healing material prepared by the method of the present invention, the healing can be promoted, the physiological repair can be promoted, the pathological repair can be inhibited, and the regeneration repair efficiency and the repair quality of the damaged tissue can be improved. The novel biological wound healing material of the present invention can be used for repairs of various human soft tissues, membranous tissues or cavity walls, and for cavity filling, and can be used as the ideal extracellular matrix biomimetic material and the cell scaffold material for wound repairs of the tissues and the organs, promotion of wound healing, prevention of adhesion and the like.

The invention discloses a compound growth factor of blood platelet sources as well as a preparation method and application of the compound growth factor. According to the compound growth factor as well as the preparation method and the application of the compound growth factor, platelet-rich plasma as the raw material is firstly subjected to virus inactivation through an S / D (Solvent / Detergent) method to remove such reagents as organic solvents and eradicators in the platelet-rich plasma and then activated by adding calcium or / and thrombin, supernatant fluid after activation is extracted to obtain the compound growth factor fitting for the natural proportion of the human body; and the problems that single recombinant growth factors in clinic are high in price, and difficult to purify, is involved into gene recombination biological safety and on the like are overcome. The invention further discloses a compound growth factor gel obtained based on the compound growth factor by adding chitosan microspheres carriers, which can slowly release growth factors during use. The compound growth factor has remarkable treatment effects for acute wounds, burns, cosmetic treatment, and chronic hard-healing wounds, is convenient to use, and has high safety.

The invention belongs to the technical field of blood products and particularly relates to a preparation method for platelet rich plasma (PRP). The method comprises a blood collection step, a lymphocyte separation medium treatment step, a first-time centrifugation step, a second-time centrifugation step and the like. According to the preparation method disclosed by the invention, on the basis of the existing PRP preparation technology, firstly, collected blood is treated by adopting a lymphocyte separation medium, and then, by optimizing a centrifugal force and time of centrifugation, the PRP concentration effect of the prepared PRP is further promoted, and the number of red blood cells is greatly reduced; the preparation method has better practical application value.

Method for separating whole blood into a leukocyte-depleted erythrocyte concentrate and thrombocyte-containing plasma, in which method whole blood is separated in a first step into an erythrocyte concentrate and plasma in a plasma filter and is then separated into a second erythrocyte concentrate and more thrombocyte-containing plasma during the return flow through the same plasma filter, the erythrocyte concentrate obtained being mixed with an additive solution, and the entire separation system being closed on itself and sterile.

The invention relates to a platelet-rich plasma rapid separating device and a method, and belongs to the field of platelet-rich plasma extracting devices and methods. The device comprises a centrifugal tube; the centrifugal tube comprises an inner tube body, an outer tube body and a sealing cover, wherein the inner tube body is sleeved with the sealing cover; the inner tube body is provided with a shrinking channel, blood is centrifuged, then suction of cell components can be avoided in the mode that the channel is closed through a buckling device, the centrifugal operation procedure is simplified, and the concentration and the preparing efficiency of the platelet-rich plasma after centrifugation are increased; meanwhile, the outer tube body of the centrifugal tube is in a centrifugal-tube shape, the centrifugal tube can be in fit with a universal centrifugal device in the market, and a platelet-rich plasma enrichment device is easy to operate, low in cost and capable of providing a high-concentration and low-cost platelet-rich-plasma and platelet-rich-gel device and method for clinical research.

A method for preparing PRP (platelet-rich plasma) of umbilical cord blood includes steps: subjecting fresh umbilical cord blood to low-speed centrifuging, removing a lower cell layer, and subjecting upper platelet containing plasma to ultrahigh-speed centrifuging until the liquid is separated into two layers including an upper plasma layer and a lower layer of precipitates which are platelets; removing the upper plasma layer, leaving a small quantity of plasma in an original centrifugal tube, well mixing, vibrating, counting the platelets, and well mixing according to a proportion that 1ml of plasma contains 1.2+ / -0.125 billion of platelets approximately. The platelet-rich plasma is effective in treatment of cartilage injuries, pain alleviation and function restoration and especially remarkable in treatment of senile osteoarthritis.

The invention belongs to the blood product technology field, and discloses a preparation method for leukocyte-depleted platelet rich plasma. The method comprises the following steps: firstly, anticoagulants and blood are drawn and mixed, and whole blood to be separated is obtained; secondly, first centrifugation is carried out, a centrifugate divided into three layers from bottom to top is obtained, and platelet and plasma components in the upper layer are obtained; thirdly, the obtained upper layer components are centrifuged again, and platelet deposition at the bottom and a plasma supernatant are formed; part of the plasma supernatant is drawn, the platelet deposition is subjected to resuspension by the left plasma supernatant, and leukocyte-depleted platelet rich plasma is prepared. The method achieves elimination of leukocytes and enrichment of platelets at the same time, operation is convenient, consumed time is short, the technical indexes of the prepared plasma product are better than technical indexes of present products with the same kind. Centrifugation for two times in the method can be carried out in two centrifuge tubes respectively, or be carried out in one centrifugal device. The invention provides a centrifugal device used for performing the preparation method.

The invention relates to platelet-rich plasma freeze-dried powder and a preparation method and application thereof, in particular to a method for extracting platelet-rich plasma from blood and preparing the free-dried powder of the platelet rich plasma. The method includes the steps of (a), placing whole blood into a container with anticoagulation agents, and evenly mixing the blood with the anticoagulation agents; (b), placing the blood in a centrifugal tube, and performing primary centrifugation to separate the blood into three layers; (c), extracting the upper layer and most of the middle layer, transferring the extracted blood into a new centrifugal tube, evenly mixing, and performing secondary centrifugation; (d), discarding the upper plasma of the centrifugal tube, enabling sedimentary blood platelets to be re-suspended by using the residual plasma to obtain the platelet-rich plasma; (e), adding freeze-drying excipients into the platelet-rich plasma, and freeze-drying the platelet-rich plasma to obtain the platelet-rich plasma freeze-dried powder. The plateletrich plasma freeze-dried powder is applicable to the fields such as the orthopaedics department, the stomatology department, dentofacial surgery, sport medicine and cosmetic medicine. The freeze-dried powder comprises the freeze-drying excipients and has an excellent technical effect.

The invention provides a stem cell injection for treating osteoarthritis cartilage defect and preparation and application thereof. The injection comprises fifth-generation umbilical cord mesenchymal stem cells and a liquid solvent, wherein the fifth generation umbilical cord mesenchymal stem cells are suspended in the liquid solvent, and the concentration is (2.0-3.0)x10<7> cells / 4.5-5.0ml of the liquid solvent. The liquid solvent is normal saline, or autologous platelet-rich plasma, or a mixed solution of normal saline and human albumin, or a mixed solution of normal saline and autologous platelet-rich plasma. The human albumin accounts for 5-8% in volume by percentage. The normal saline accounts for 10% or below in volume by percentage. The injection is administered by intravenous injection or bone joint cavity local injection or the combination of the two, the cell injection amount of the intravenous injection is (1.0-1.5)x10<8> cells / one time, and the cell injection amount of the bone joint cavity injection is (2.0-3.0)x10<7> cells / one time / one knee. The effect of the stem cell injection for treating the integrity degree of osteoarthritis cartilage is obviously superior to that of a traditional hyaluronic acid (HA) method.

The invention belongs to the technical field of preparation of platelet rich plasma, and in particular discloses a method for preparing PRP (Platelet Rich Plasma) through a manual two-step centrifugation method. The method comprises the following steps: centrifuging 25 ml of whole blood mixed with an anticoagulant for 6 minutes at centrifugal force of 800 g for the first time by adopting the two-step centrifugation method, absorbing plasma on the upper layer and on boundary layer in the layered whole blood, and arranging the plasma in an another centrifugal tube; centrifuging for the second time, namely centrifuging for 5 minutes at centrifugal force of 1400 g, and absorbing about 4 ml of the layered plasma on the middle layer to obtain the platelet rich plasma. According to the PRP prepared by the technology, the platelet concentration is 886.33*10<9> / L, the mean corpuscular hemoglobin in the PRP is 27.72 g / L, and the clinical treatment requirements are met.

The human skin filler for injection consists of cell components, blood plasma with rich thrombocyte, blood coagulation factor and calcium ion. Each milliliter of the filler for injection contains cell components in (10-50)x10<6>, thrombocyte in (100-700)x10<6>, blood coagulation factor in 1-10 U, and calcium ion in 10-40 mmol. The cell components are at least one of corium stem fibroblast, fibroblast precursor and fibroblast obtained through acquiring the skin from the patient, extracorporeal no-serum culturing and amplifying, and collecting; the thrombocyte is extracted from the autoblood of the patient; and the blood coagulation factor is autogenous or extraneous. The present invention also provides preparation process of human soft tissue filler for injection. The present invention has the advantages of quick obvious filling and repairing effect, last curative effect, high safety, etc.

The invention is suitable for the technical field of biomedicine materials and provides a biological scaffold containing platelets and hyaluronic acid which are mixed uniformly, wherein the platelets are activated by calcium ions before being mixed with hyaluronic acid. The invention also provides a preparation method of the biological scaffold. The preparation method comprises the following steps: obtaining platelet-rich plasma (PRP); adding calcium ions into the PRP; and uniformly mixing the obtained PRP with hyaluronic acid. The biological scaffold provided by the invention has good biological compatibility and more pore structures and can ensure that the platelets slowly release growth factors.

This invention relates to a method of reducing residual white blood cells in an apheresed platelet product. The method includes the steps of adding to the platelet product a solution comprising sodium chloride and magnesium and inducing degradation of the residual white blood cells.

The invention discloses a hydrogel loaded with platelet-rich plasma and antibacterial peptide as well as a preparation method and application of the hydrogel. The preparation method comprises the following steps: grafting antibacterial peptide into sodium hyaluronate to form a compound of sodium hyaluronate and antibacterial peptide, then uniformly mixing the compound solution of the sodium hyaluronate and the antibacterial peptide with platelet-rich plasma, and then mixing the obtained mixture with a dextran oxide solution to form the hydrogel loaded with the platelet-rich plasma and the antibacterial peptide. The hydrogel prepared by the invention is biodegradable, has good mechanical properties and biocompatibility, can promote wound healing and tissue regeneration, has a long-acting drug-resistance-free antibacterial effect, and can be applied to wound repair of chronic skin defects.

A method for more effectively concentrating blood platelets for use in medical procedures includes providing preparation and concentrating tubes. Anticoagulated whole blood is added to the preparation tube, which is centrifuged to separate red blood cells from a platelet plasma suspension The platelet plasma suspension is aspirated and loaded into the concentrating tube, which is itself centrifuged to separate the platelet plasma suspension into platelet poor plasma and platelet rich plasma layers. The platelet poor plasma is aspirated through a port of the concentrating tube and the remaining concentrated PRP is aspirated through the same or a different port of the concentrating tube.

The invention discloses a platelet-rich gel and a preparation method thereof. The preparation method comprises the following steps that an ethylenediamine tetracetic acid sodium solution is prepared,specifically, sterile water for injection is taken, sodium bicarbonate is added into the sterile water, the pH value of the sterile water for injection is adjusted to 7.8, then ethylenediamine tetracetic acid sodium is added into the mixture and mixed evenly, and the ethylenediamine tetracetic acid sodium solution with the molarity being 0.25 mol / L is prepared; peripheral venous blood of a patientis collected by a container containing the ethylenediamine tetracetic acid sodium solution, centrifugation is conducted, and platelet-rich plasma is prepared; thrombin lyophilized powder and calciumgluconate with the mass concentration being 10% are taken, and the thrombin lyophilized powder and the calcium gluconate are mixed evenly according to the ratio of 1ml of 10% calcium gluconate for every 1000 U of the thrombin lyophilized powder, and a thrombin solution is prepared. The platelet-rich plasma is prepared by mixing the platelet-rich plasma and the thrombin solution according to the volume ratio of 10:1; and the platelet-rich plasma and the thrombin solution are mixed evenly according to the volume ratio of 10:1, and the platelet-rich gel is prepared. According to the platelet-richgel, the therapeutic effect is good, and wound healing can be effectively promoted, and treatment time is shortened.

The invention provides a composition, the application thereof and a preparation. The composition comprises a component A and a component B. The preparation comprises a first preparation and a second preparation, wherein the first preparation comprises hyaluronidase and a solvent acceptable on medicine, the second preparation comprises autologous platelet-rich plasma, an autologous platelet-rich plasma activator, cornua cervi pantotrichum essence, vitamin C, a persimmon leaf extracting solution and hyaluronic acid. The hyaluronidase in the first preparation improves nutrient substance infiltration and diffusion, the second preparation can directly repair damaged skin, remove melanin and harmful free radical, and the face skin becomes delicate and white; the stability of later treatment period can be well kept by means of the moisturizing function of the hyaluronic acid, so that the skin whitening effect is obvious, and the holding time is long.

The invention belongs to the technical field of cosmetology, in particular to a formula of mini-needle nutrient solutions having an autogenous repairing function. The formula of the mini-needle nutrient solutions having the autogenous repairing function comprises the following components in parts by mass: 35 parts to 45 parts of autogenous collagen, and also comprises 35 parts to 45 parts of stemcell suspensions, 3 parts to 8 parts of hyaluromic acid solutions, 3 parts to 8 parts of epidermal growth factor solutions, 3 parts to 8 parts of vitamin E solutions and 3 parts to 8 parts of antioxidant molecules; the formula also comprises 20 parts of platelets-rich plasma, 5 parts of hydroxyapatites, 5 parts of polylactides, 5 parts of polynucleotide, and 10 parts of fibroblast. The formula ofthe mini-needle nutrient solutions having the autogenous repairing function disclosed by the invention has the advantages that the formula is reasonably configured, the functions are varied, the autogenous repairing capability of the skin can be effectively activated, the nutrient solutions are safe and effective, risks are avoided, and the nutrient solutions can be applied from face to body, andare fully coverage of referred items.