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Hydrogel loaded with platelet-rich plasma and antibacterial peptide as well as preparation method and application of hydrogel

A platelet-rich plasma and antimicrobial peptide technology, which is used in medical science, bandages, etc. to promote tissue regeneration, good mechanical properties and biocompatibility, and promote wound healing.

Inactive Publication Date: 2020-03-13
壹齐生物科技(广州)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few studies using AMPs as wound dressings

Method used

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  • Hydrogel loaded with platelet-rich plasma and antibacterial peptide as well as preparation method and application of hydrogel
  • Hydrogel loaded with platelet-rich plasma and antibacterial peptide as well as preparation method and application of hydrogel
  • Hydrogel loaded with platelet-rich plasma and antibacterial peptide as well as preparation method and application of hydrogel

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] The preparation of embodiment 1 ODEX

[0048] Dissolve 1g of DEX in 10mL of pure water to prepare a dextran aqueous solution with a concentration of 10w / v%, then add 2mL of a sodium periodate solution with a concentration of 100mg / mL and place it under dark conditions at room temperature for oxidation reaction for 20h; then Add 2 mL of ethylene glycol into the reaction solution, neutralize excess sodium periodate, and stop the oxidation reaction; place the reaction solution in deionized water for dialysis with a cellulose dialysis bag with a molecular weight cut-off of 8-15 kDa for 3 days, and freeze-dry it. Get ODEX.

Embodiment 2

[0049] Preparation and infrared spectroscopic analysis of embodiment 2 ODEX

[0050] Dissolve 5g of DEX in 25mL of pure water to prepare a DEX aqueous solution with a concentration of 20w / v%, then add 10mL of sodium periodate solution with a concentration of 20mg / mL and place it under room temperature and dark conditions for oxidation reaction for 10h; subsequently, the 10mL of ethylene glycol was added to the reaction solution, and the oxidation reaction was stopped after neutralizing excess sodium periodate; the reaction solution was dialyzed in deionized water for 3 days with a cellulose dialysis bag with a molecular weight cut-off of 8-15kDa, and then freeze-dried to obtain ODEX.

[0051] The DEX and the ODEX characteristic peak changes of this embodiment were characterized by Fourier transform infrared spectroscopy. Get the sample of DEX and ODEX of 1-2mg respectively and put in agate mortar and grind, then add the Potassium Bromide powder (the ratio of the sample to be ...

Embodiment 3

[0053] The preparation of embodiment 3 aminated sodium hyaluronate

[0054] The preparation method of the aminated sodium hyaluronate of the present embodiment comprises the following steps:

[0055] (1) Dissolve 500mg HA in 125mL pure water to form a 4mg / mL HA aqueous solution, then add 8g ADH into the HA aqueous solution, and magnetically stir for about 4 hours to obtain a reaction solution;

[0056] (2) Dissolve 750mg EDC and 600mg HoBt in 10mL of DMSO / H 2 O solution, DMSO / H 2 DMSO and H in O solution 2 The volume ratio of O is 1:1, and then the pH of the above solution is adjusted to 4.75 using NaOH and HCl solutions to obtain a mixed solution;

[0057] (3) Add the mixed solution to the reaction solution, keep the reaction for about 4 hours, then adjust the pH to 7.0 to complete the reaction;

[0058] (4) Place the mixed solution after the above-mentioned completed reaction into 100mM NaCl, 25% ethanol, and deionized water for dialysis for one day, change the dialysate...

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Abstract

The invention discloses a hydrogel loaded with platelet-rich plasma and antibacterial peptide as well as a preparation method and application of the hydrogel. The preparation method comprises the following steps: grafting antibacterial peptide into sodium hyaluronate to form a compound of sodium hyaluronate and antibacterial peptide, then uniformly mixing the compound solution of the sodium hyaluronate and the antibacterial peptide with platelet-rich plasma, and then mixing the obtained mixture with a dextran oxide solution to form the hydrogel loaded with the platelet-rich plasma and the antibacterial peptide. The hydrogel prepared by the invention is biodegradable, has good mechanical properties and biocompatibility, can promote wound healing and tissue regeneration, has a long-acting drug-resistance-free antibacterial effect, and can be applied to wound repair of chronic skin defects.

Description

technical field [0001] The invention relates to the technical field of biomedical materials, in particular to a hydrogel loaded with platelet-rich plasma and antibacterial peptides, a preparation method and application thereof. Background technique [0002] Wound treatment is one of the most important and challenging health problems. In the United States, 6.5 million patients suffer from chronic trauma, costing $25 billion a year to treat it. Without proper treatment, skin wounds often become infected with bacteria that prolong inflammation, disrupt re-epithelialization, inhibit collagen production, and delay wound healing. Due to the loss of its self-protection mechanism, the damaged skin accumulates interstitial fluid, leading to a large number of invasion and reproduction of pathogens and microorganisms at the wound, which leads to severe wound infection, delays the wound healing process, and causes wound ulceration. Furthermore, once bacteria attach to the wound surfac...

Claims

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Application Information

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IPC IPC(8): A61L26/00
CPCA61L26/0057A61L26/0066A61L26/008A61L26/009A61L2300/252A61L2300/404A61L2300/412C08L5/08
Inventor 李建慧程柳柳
Owner 壹齐生物科技(广州)有限公司
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