Preparation method and device for leukocyte-depleted platelet rich plasma

Abstract

The invention belongs to the blood product technology field, and discloses a preparation method for leukocyte-depleted platelet rich plasma. The method comprises the following steps: firstly, anticoagulants and blood are drawn and mixed, and whole blood to be separated is obtained; secondly, first centrifugation is carried out, a centrifugate divided into three layers from bottom to top is obtained, and platelet and plasma components in the upper layer are obtained; thirdly, the obtained upper layer components are centrifuged again, and platelet deposition at the bottom and a plasma supernatant are formed; part of the plasma supernatant is drawn, the platelet deposition is subjected to resuspension by the left plasma supernatant, and leukocyte-depleted platelet rich plasma is prepared. The method achieves elimination of leukocytes and enrichment of platelets at the same time, operation is convenient, consumed time is short, the technical indexes of the prepared plasma product are better than technical indexes of present products with the same kind. Centrifugation for two times in the method can be carried out in two centrifuge tubes respectively, or be carried out in one centrifugal device. The invention provides a centrifugal device used for performing the preparation method.

Description

technical field [0001] The invention belongs to the technical field of blood products, and in particular relates to a preparation method of blood plasma products and a centrifugal device for implementing the method. Background technique [0002] Platelet-rich plasma (PRP) is a blood product rich in platelets. According to whether it contains leukocytes, it can be roughly divided into two categories: L-PRP rich in leukocytes and P-PRP without leukocytes. In recent years, a large number of studies have confirmed that the active ingredient in PRP that promotes the repair and regeneration of bone tissue, cartilage tissue and tendon tissue is platelets, and the leukocytes in PRP not only do not promote tissue repair; The local immune response can inhibit tissue repair, thereby reducing the clinical efficacy of PRP and increasing the incidence of adverse reactions such as pain at the injection site. Therefore, the preparation method of leukocyte-depleted platelet-rich plasma is t...

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Problems solved by technology

[0003] There are three main indicators to evaluate the preparation method of P-PRP: first, the clearance rate of leukocytes, the higher the clearance rate, the more thorough the clearance of leukocytes, which can improve the clinical efficacy and reduce adverse reactions; secondly, the recovery rate of platelets, the higher the recovery rate, the blood The higher the degree of utilization; the second is the degree of platelet enrichment, only the onset dose of PRP is 4 to 6 times the degree of enrichment, too low degree of enrichment will lead to a decline in efficacy, resulting in insignificant clinical efficacy

However, due to the serious overlap between the distribution area of ​​leukocytes and the distribution area of ​​platelets, according to the current separation technology, the removal of more leukocytes will inevitably lead to the recovery of fewer platelets, so the leukocyte clearance rate and platelet recovery rate seem to be a contradiction.

[0004] At present, the Anitua method is mostly used to prepare PRP clinically, and there is also a method according to the Biomet company of the United States. The method provided by the platelet-rich plasma preparation kit prepares PRP, but the indicators of the prepared PRP are not ideal

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