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Method for preparing platelet rich plasma through manual two-step centrifugation method

A platelet-rich plasma, secondary centrifugation technology, applied in separation methods, chemical instruments and methods, liquid separation, etc., can solve the problems of unstable PRP quality, high cost of equipment set preparation technology, low treatment costs, easy promotion, Less time consuming effect

Inactive Publication Date: 2014-01-15
广东威尔医院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The present invention provides a method for preparing PRP by manual secondary centrifugation in order to overcome the problems of unstable quality of PRP prepared by the existing manual method and high cost of equipment package preparation technology.

Method used

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  • Method for preparing platelet rich plasma through manual two-step centrifugation method
  • Method for preparing platelet rich plasma through manual two-step centrifugation method
  • Method for preparing platelet rich plasma through manual two-step centrifugation method

Examples

Experimental program
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Effect test

Embodiment 1

[0027] S1. Materials and methods

[0028]Main equipment and reagents: eppendorf5702 centrifuge, centrifuge tube, EDTA blood collection tube, syringe, heparin sodium, automatic blood cell analyzer.

[0029] S2. Subjects: patients who intend to voluntarily receive PRP injection therapy. Screening criteria for subjects:

[0030] (1) The results of blood cell analysis of the patient's venous blood were within the normal range of the three lines: the platelet count was 100-300×10 9 / L, hemoglobin for women is between 135-150g / L, for men is between 140-155g / L, white blood cell count is 4-10×10 12 / L between.

[0031] (2) The erythrocyte sedimentation rate and C-reactive protein were within the normal range.

[0032] (3) Those who have no symptoms of infection in the whole body and treatment area.

[0033] (4) The basic condition is good, without hyperlipidemia, diabetes and other diseases outside the blood system that greatly affect blood components.

[0034] (5) P...

Embodiment 2

[0057] After exploring the conditions of Example 1, in this example, 6 patients described in Example 1 were used to prepare platelet-rich plasma using the method of the present invention.

[0058] Collect 25mL of peripheral venous blood from the patient into a 50mL centrifuge tube, add 0.5mL heparin for anticoagulation, put it into the centrifuge after balancing, and perform the first centrifugation according to the set time (6 minutes) and centrifugal force (800g); then take out the centrifuge tube, draw Put the upper and boundary layer plasma in the venous blood after stratification into another clean centrifuge tube; after balancing, put it into the centrifuge for the second centrifugation according to the set time (5 minutes) and centrifugal force (1400g); then take out the centrifuge tube , draw about 4ml of the stratified middle plasma, which is platelet-rich plasma. The whole process only needs a high-speed automatic centrifuge and one pipetting.

[0059] According to ...

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Abstract

The invention belongs to the technical field of preparation of platelet rich plasma, and in particular discloses a method for preparing PRP (Platelet Rich Plasma) through a manual two-step centrifugation method. The method comprises the following steps: centrifuging 25 ml of whole blood mixed with an anticoagulant for 6 minutes at centrifugal force of 800 g for the first time by adopting the two-step centrifugation method, absorbing plasma on the upper layer and on boundary layer in the layered whole blood, and arranging the plasma in an another centrifugal tube; centrifuging for the second time, namely centrifuging for 5 minutes at centrifugal force of 1400 g, and absorbing about 4 ml of the layered plasma on the middle layer to obtain the platelet rich plasma. According to the PRP prepared by the technology, the platelet concentration is 886.33*10<9> / L, the mean corpuscular hemoglobin in the PRP is 27.72 g / L, and the clinical treatment requirements are met.

Description

technical field [0001] The invention relates to a method for preparing platelet-rich plasma, in particular to a method for preparing platelet-rich plasma by manual secondary centrifugation. Background technique [0002] Platelet-rich plasma (platelet-rich plasma, PRP) is a plasma product derived from human platelet cells, and its platelet cell concentration standard was first proposed by Professor Marx of the University of Miami in 2001. He believed that the platelet concentration in PRP should be whole More than 5 times the platelet concentration in the blood. However, the PRP prepared by most researchers is difficult to meet this standard, and they found in the study that even if the platelet concentration in PRP is slightly lower than this standard, good therapeutic effect can still be obtained. In 2011, Canadian scholar Drew W.T. proposed a new standard—the platelet concentration in PRP is 3 to 5 times that in whole blood, and this standard is recognized by most people....

Claims

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Application Information

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IPC IPC(8): B01D17/038
Inventor 林子洪郑秋坚沈梓维
Owner 广东威尔医院有限公司
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