149 results about "PLT - Platelets" patented technology

1. A method for classifying and counting leukocytes, which comprises:(1) a step of staining cells in a sample obtained from a hematological sample by treatment with a hemolytic agent, with a fluorescent dye;(2) a step of introducing the sample containing the stained cells into a flow cytometer to measure first scattered light, second scattered light different from the first scattered light and fluorescence of the respective cells;(3) a step of obtaining scattered light peak intensities and scattered light widths of the respective cells based on the measured first scattered light, obtaining scattered light intensities of the respective cells based on the measured second scattered light, and obtaining fluorescece intensities of the respective cells based on the measured fluorescence light;(4) a step of classifying the cells into a first group and a second group based on the scattered light peak intensities and the scattered light widths, the first group including leukocytes and second group including platelet clumps;(5) a step of classifying the leukocytes included in the first group into at least lymphocytes, monocytes and granulocytes based on the scattered light intensities and the fluorescence intensities; and(6) a step of counting the classified lymphocytes, the classified monocytes and the classified granulocytes.

A flexible, disposable centrifuge bag for receiving and holding a fluid sample, comprising one or more tubes and upper and lower flexible sheets having doughnut shaped configurations. The upper and lower sheets are superimposed and completely sealed together at their outer perimeters to define an outer perimeter of the centrifuge bag. The inner perimeter of the bag defines a central core. The tubes are sandwiched between the upper and lower sheets and extend from the central core of the centrifuge bag. The upper and lower sheets are sealed together at their inner perimeters such that the tubes are sealed between said upper and lower sheets at the inner perimeter. The bag may be used to harvest platelet rich plasma from whole blood in either a batch method or while simultaneously infusing additional aliquots of whole blood into the bag during centrifugation.

The invention concerns a method for the detection of the presence and / or the severity of a liver disease in a patient comprising measuring in an isolated sample TIMP-1 (Tissue Inhibitor of Metalloproteinase I), A2M (a-2-macroglobulin), PLT (number of blood plateletes, PI (prothrombin index), optionally at least one additional parameter selected from the group consisting of urea and GGT (γ-glutamyltranspeptidase) and optionally measuring at least one additional biochemical or clinical parameter and diagnosing the presence and / or severity of a liver disease based on the presence or measured levels of these parameters. The method can be used for monitoring therapeutic treatment of liver fibrosis and staging of liver fibrosis.

The invention relates to a blood cell image detection and counting method based on a convolutional neural network, and belongs to the field of medical image processing, and the method comprises the steps: dividing an obtained blood cell image data set into a training set, a verification set and a test set, and carrying out the enhancement of the blood cell image data set through employing a Mosaic algorithm; inputting the image into a CSPDarkNet53 network to obtain a plurality of feature maps, and transmitting the feature maps into a Neck network to extract fusion features; predicting the blood cell image by using an improved YOLOv4 object detection algorithm; performing confidence score sorting on the prediction frame, and obtaining a finally displayed prediction frame through a non-maximum suppression algorithm; then counting the blood cells by using the predictive tags of the cells; the prediction result of the detection model being verified again by adopting KNN and DIOU, and the problem of platelet repeated detection being eliminated. The method realizes accurate and rapid detection and counting of blood cells, and has great practical application value.

The invention discloses a PLT particle detection method and device for a blood cell analyzer, and the method comprises the steps: carrying out the classification of a pulse signal according to a plurality of classification precisions, obtaining a plurality of classification histograms with the classification precisions, segmenting the classification histogram with each classification precision into a preset number of reference sub-classification histograms according to a reference boundary, correcting the reference boundary in each reference sub-classification histogram to obtain an accurate sub-classification histogram, determining the number of platelet aggregation particles based on the corresponding sub-classification histograms in all the classification histograms, determining an accurate first boundary according to a preset display histogram function and the number of platelet aggregation particles, so that the platelet aggregation particles and the red blood cell particles can be distinguished more accurately in the classification histogram.

The invention relates to an improved self platelet-rich plasma preparation method. A centrifugation method is adopted two times, centrifugation is performed under the condition of 850-1875 g for 11-15minutes for the first time, and centrifugation is performed under the condition of 100 g for 12 minutes for the second time. The method is simple, the platelet recovery rate is high, the cost is low,the preparation process is closed, the pollution risk is minimized, the product quantity and platelet number can be flexibly adjusted according to clinic demands, and a new method is provided for clinic preparation of self platelet-rich plasma.

A method for enumerating white blood cells and nucleated red blood cells. The steps of the method are as follows:(a) providing a lysed sample of whole blood;(b) introducing the lysed sample to a light-scattering multi-angle depolarizing flow cytometer;(c) removing depolarizing interference, e.g., lipid droplets and other measured particles;(d) differentiating nucleated red blood cells and noise from white blood cells in the absence of depolarizing interference;(e) differentiating nucleated red blood cells from noise in the absence of depolarizing interference and white blood cells; and(f) differentiating possible platelet clumps.

The present invention disclosed a reagent that stabilizes blood sample cells, including the following concentration materials: 100 μmol / lβ-cycloped, 100 μmol / L di-sodium glycolic acid, 0.05%potassium chromate, 50 μmol / L triterfluilow,100 μmol / L Truquan, 11mmol / L citric acid, 1.5mmol / L catechine, 20 μmol / L ductoid Damo, 0.37mmol / L adenosine, 20mmol / L glucose, 1.25mmol / L?PEG.The present invention can stabilize blood cells and prevent blood plates from gathering and stabilize blood cells for at least 36 hours. The treated blood samples will not affect subsequent sample molecular analysis. In addition to being widely used in blood sample molecular analysis and testing, it is also suitable for sample cells to be used for sample cellsCells or molecular detection and analytical technology platforms with strict requirements, such as micro -current control technology, streaming cytometer, etc.

The invention provides a household blood sample testing device and system, which belong to the field of medical technologies. The device includes a blood collection module, a red blood cell detectionmodule, a white blood cell detection module, a platelet detection module, a CRP detection module, a control module, a database, and a report module. The control module controls the blood collection module to perform multiple blood collections according to the number of detection modules. The blood collected each time is stored in a reagent tube. Reagent tubes after blood collection are delivered to each detection module. The database stores a normal value comparison table of blood cells and a CRP normal value comparison table. The report module forms a test report by comparison with the database based on the detection results of detection modules. The system includes a household blood sample testing device and an inquiry module arranged on a doctor side. The household blood sample testingdevice sends the disease condition and the report / detection results to the inquiry module. The inquiry module feeds back inquiry information to a user terminal. According to the invention, the deviceand system are convenient for home detection and online inquiry and used for routine detection, which saves the time of hospital visits and relieves the work pressure of doctors.

The present invention relates to blood sampler and the preparation of blood samples that can be used for not only for blood gas, basic metabolic panel parameter analysis but also for a platelet countand / or white blood count, such as a 3-diff or 5-diff. The blood samples comprise at least one anticoagulant for the determination of blood gas and basic metabolic panel parameters and at least one anti-platelet agent.

The invention is a method for coagulation related function detection by EDTA (ethylene diamine tetraacetic acid) anticoagulation, and belongs to the field of medical in vitro diagnosis industry. According to the method, the EDTA anticoagulation can be used for blood cell count by a blood analyser, and can be used for detection of platelet aggregation and other blood coagulation functions.