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151 results about "PLT - Platelets" patented technology

The PLT blood test is also known as the platelet blood test or a platelet count. PLT in blood test stands for 'platelet'. PLT testing is done to count how many blood platelets there are in our blood. The number of platelets in one microliter of blood can range from 150,000 to 400,000.

Method and apparatus for reflected imaging analysis

Method and apparatus for reflected imaging analysis. Reflected imaging is used to perform non-invasive, in vivo analysis of a subject's vascular system. A raw reflected image (110) is normalized with respect to the background to form a corrected reflected image (120). An analysis image (130) is segmented from the corrected reflected image to include a scene of interest for analysis. The method and apparatus can be used to determine such characteristics as the hemoglobin concentration per unit volume of blood, the number of white blood cells per unit volume of blood, a mean cell volume, the number of platelets per unit volume of blood, and the hematocrit. Cross-polarizers can be used to improve visualization of the reflected image.
Owner:INTPROP MVM

Apparatus for differentiating blood cells using back-scatter

Blood cells of interest are readily distinguishable from other blood cells and look-a-like particles found in a blood sample by their back-scatter signature. A preferred method for differentiating platelets in a blood sample is to irradiate the cells and particles, one at a time, with a beam of radiation, and to detect back-scattered (reflected) radiation using a plurality of optical fibers to transmit the back-scattered radiation to a high-gain photodetector, e.g. a photomultiplier tube. Preferably, the back-scatter signal so obtained is combined with a second signal representing, for example, either the level of forward-scatter within a prescribed, relatively narrow angular range, or the level of side-scattered radiation, or the level of attenuation of the cell-irradiating beam caused by the presence of the irradiated cell or particle in the beam, or the electrical impedance of the irradiated cell or particle, to differentiate the cells of interest. The method and apparatus of the invention are particularly useful in differentiating platelets and basophils in a blood sample.
Owner:COULTER INTERNATIONAL CORPORATION

Automated microscopic cell analysis

Disclosed in one aspect is a method for performing a complete blood count (CBC) on a sample of whole blood by metering a predetermined amount of the whole blood and mixing it with a predetermined amount of diluent and stain and transferring a portion thereof to an imaging chamber of fixed dimensions and utilizing an automated microscope with digital camera and cell counting and recognition software to count every white blood cell and red blood corpuscle and platelet in the sample diluent / stain mixture to determine the number of red cells, white cells, and platelets per unit volume, and analyzing the white cells with cell recognition software to classify them.
Owner:MEDICA CORP

Platelet aggregation using a microfluidics device

A microfluidics device to provide real time monitoring of platelet aggregation of a biological sample obtained from a subject. The device comprises a channel configured for passage of the biological sample, the channel comprising a protrusion configured to induce an upstream region of shear acceleration coupled to a downstream region of shear deceleration and defining there-between a region of peak rate of shear, the downstream region of shear deceleration defining a zone of platelet aggregation. The device further comprises a platelet detection means for detecting aggregation of platelets in the zone of aggregation as a result of passage of the biological sample through the channel. Methods to assess real time platelet aggregation of a biological sample obtained from a subject are further described.
Owner:MONASH UNIV +1

Method of classifying and counting leucocytes

1. A method for classifying and counting leukocytes, which comprises:(1) a step of staining cells in a sample obtained from a hematological sample by treatment with a hemolytic agent, with a fluorescent dye;(2) a step of introducing the sample containing the stained cells into a flow cytometer to measure first scattered light, second scattered light different from the first scattered light and fluorescence of the respective cells;(3) a step of obtaining scattered light peak intensities and scattered light widths of the respective cells based on the measured first scattered light, obtaining scattered light intensities of the respective cells based on the measured second scattered light, and obtaining fluorescece intensities of the respective cells based on the measured fluorescence light;(4) a step of classifying the cells into a first group and a second group based on the scattered light peak intensities and the scattered light widths, the first group including leukocytes and second group including platelet clumps;(5) a step of classifying the leukocytes included in the first group into at least lymphocytes, monocytes and granulocytes based on the scattered light intensities and the fluorescence intensities; and(6) a step of counting the classified lymphocytes, the classified monocytes and the classified granulocytes.
Owner:SYSMEX CORP

Platelet oscillation storage box

The invention discloses a platelet oscillating storage box, which includes a box body. The box body is provided with six circular array and connected rotating cavities. There are five storage blocks in the rotating cavity, and the storage blocks are provided with storage. A cavity, a storage block is fixed on the lower end wall of the storage cavity, and an end of the storage cavity close to the center of the array is provided with a fixed block that is symmetrical up and down, and a movable clamping block is provided between the fixed blocks. The clamping device can be used to clamp the test tube containing the platelets in the storage cavity, and the storage cavity is independent and communicates with the thermostat independently. When taking or storing platelets, the thermostatic conditions in the storage cavity to be placed are blocked. Cut off, reducing energy loss, without destroying the constant temperature environment in other storage chambers, and prolonging the storage time of platelets. Secondly, the sealing device can keep the storage chamber sealed, and the sealing cover and the storage chamber remain locked during the oscillation process. Ensure constant temperature in the storage chamber.
Owner:QIAN GUOCHENG

System for estimating state of critically ill patient in obstetrical department

The invention discloses a system for estimating the state of a critically ill patient in an obstetrical department, which comprises an input end, an output end and a data processing module. Firstly, measured heart rate, blood pressure, body temperature, breathing rate, pH, blood HCO-3 concentration, oxygen partial pressure, alveolar arterial blood oxygen tension difference, potassium ion concentration, hematokrit, white cell count, platelet count, fibrinogen, glutamic-oxaloacetic transaminase, albumin, bilirubin, creatinine, blood glucose concentration and nervous system grading data are input the system through the input end; the data processing module evaluates a death risk factor; and at last, the output end directly reflects the result of the patient and the expert suggestion. The invention can not only accurately evaluate the critical degree of the state of the patient and predict the death risk, but also can provide quantitative indexes for changing to a three-class hospital, and is beneficial to improve the treatment level of the critical state of the obstetrical department and lower the death rate of pregnant and lying-in women and perinatal infants.
Owner:陈敦金 +1

Sepsis early prediction method based on machine learning

The invention discloses a sepsis early prediction method based on machine learning. The method comprises the following steps: firstly, extracting clinical data, including demographic statistics (suchas age and gender), vital sign variables (such as heart rate and systolic pressure) and laboratory measurement indexes (such as creatinine and platelet count), of a patient within 24 hours after a patient enters an ICU by utilizing an electronic medical record, preprocessing the data, inputting the preprocessed data into an improved deep forest algorithm model for training, and outputting the disease probability of the patient after training optimization. And meanwhile, the algorithm model can also sort characteristic variables and output an early warning factor which has great influence on early prediction of sepsis. Finally, corresponding variables of the patient needing to be predicted are input into the trained model, so that early prediction of sepsis can be carried out on the patient. According to the invention, early prediction is carried out on sepsis based on a machine learning method, doctors can be assisted in making clinical decisions, and the prediction accuracy is improved.
Owner:NANJING UNIV OF AERONAUTICS & ASTRONAUTICS

Integrated kit for separating blood and concentrating prp and method for extracting prp using the same

An integrated kit for separating blood and concentrating platelet rich plasma includes (a) a main body includes an upper storage portion, a lower storage portion, and a passage portion for connecting the upper storage portion and the lower storage portion; (b) a upper cover coupled to an upper end of the upper storage portion; (c) an inner stopper inserted into a lower end of the lower storage portion so as to seal the lower storage portion; (d) a closing adjustment screw which can move vertically while penetrating a hole formed at the inner stopper and seals the passage portion; and (d) a lower cover coupled to an outside of the lower end of the lower storage portion and the outside of the inner stopper to prevent the separation of the closing adjustment screw coupled to the inner stopper in case of centrifugation.
Owner:RMEDICA

Flexible centrifuge bag and methods of use

A flexible, disposable centrifuge bag for receiving and holding a fluid sample, comprising one or more tubes and upper and lower flexible sheets having doughnut shaped configurations. The upper and lower sheets are superimposed and completely sealed together at their outer perimeters to define an outer perimeter of the centrifuge bag. The inner perimeter of the bag defines a central core. The tubes are sandwiched between the upper and lower sheets and extend from the central core of the centrifuge bag. The upper and lower sheets are sealed together at their inner perimeters such that the tubes are sealed between said upper and lower sheets at the inner perimeter. The bag may be used to harvest platelet rich plasma from whole blood in either a batch method or while simultaneously infusing additional aliquots of whole blood into the bag during centrifugation.
Owner:ARTERIOCYTE MEDICAL SYST

Device and method for measuring coagulation time and platelet activity

The present invention refers to a novel device for measuring coagulation time and platelet activity wherein the patient can measure his or her coagulation time and platelet activity without the aid of medical professionals due to the fact that this device is fully autonomous. A blood sample (14) is deposited in the dish (3) and reacted with a reactant (16). The display (9) then shows the coagulation time and platelet activity of the patient.
Owner:BOTE BOTE ANTONIO

Analysis of biological samples utilizing a coated solid phase

The invention disclosed herein comprises methods for analyzing a biological sample, such as undiluted or diluted whole blood, as well as fractions thereof, for the presence or absence and / or the concentration of disease-specific and / or other medical condition-specific markers. Such markers may include platelet activation and coagulation activation markers. The methods may comprise combining the biological sample with a coated solid phase and analyzing for the presence or absence and / or the concentration of the markers. The analysis may be performed either before or after separation of the solid phase from the biological sample. The analysis may be performed on the combined components or on any of the separated components. A preferred solid phase may be paramagnetic microparticles coated with antibodies or proteins specific for platelet activation and / or coagulation activation markers.
Owner:HENRY MICHAEL R +2

Method for preparing platelet rich plasma through manual two-step centrifugation method

InactiveCN103505911AReduce manufacturing costLess cost of clinical treatmentLiquid separationPlatelets bloodBlood plasma
The invention belongs to the technical field of preparation of platelet rich plasma, and in particular discloses a method for preparing PRP (Platelet Rich Plasma) through a manual two-step centrifugation method. The method comprises the following steps: centrifuging 25 ml of whole blood mixed with an anticoagulant for 6 minutes at centrifugal force of 800 g for the first time by adopting the two-step centrifugation method, absorbing plasma on the upper layer and on boundary layer in the layered whole blood, and arranging the plasma in an another centrifugal tube; centrifuging for the second time, namely centrifuging for 5 minutes at centrifugal force of 1400 g, and absorbing about 4 ml of the layered plasma on the middle layer to obtain the platelet rich plasma. According to the PRP prepared by the technology, the platelet concentration is 886.33*10<9> / L, the mean corpuscular hemoglobin in the PRP is 27.72 g / L, and the clinical treatment requirements are met.
Owner:广东威尔医院有限公司

Optimized automation-adaptable platelet aggregation function inspection and analysis method

The invention provides an optimized automation-adaptable platelet aggregation function inspection and analysis method, and belongs to the field of clinical laboratory medicine. The method is an improvement and optimization of a platelet aggregation function inspection and analysis method using an automation counting method. The method is suitable for platelet aggregation function automatic analysis instruments using the counting method. According to the method, the number of platelets is inspected and recorded and an aggregation rate is calculated. A laboratory test result shows that the method is better in repeatability of the obtained result of platelet aggregation rate compared with a previous largest aggregation rate method.
Owner:SINNOWA MEDICAL SCI & TECH

Method for diagnosing liver fibrosis

The invention concerns a method for the detection of the presence and / or the severity of a liver disease in a patient comprising measuring in an isolated sample TIMP-1 (Tissue Inhibitor of Metalloproteinase I), A2M (a-2-macroglobulin), PLT (number of blood plateletes, PI (prothrombin index), optionally at least one additional parameter selected from the group consisting of urea and GGT (γ-glutamyltranspeptidase) and optionally measuring at least one additional biochemical or clinical parameter and diagnosing the presence and / or severity of a liver disease based on the presence or measured levels of these parameters. The method can be used for monitoring therapeutic treatment of liver fibrosis and staging of liver fibrosis.
Owner:ROCHE DIAGNOSTICS OPERATIONS INC

Alarm method, system and storage medium for abnormalities of sample analyzer

A method, system and storage medium for providing an alarm for indicating that an abnormality is present in a sample analyzer are provided. The method includes: mixing a first aliquot of a blood sample with a diluent agent to prepare a first test sample; mixing a second aliquot of the blood sample with a lytic reagent to prepare a second test sample; detecting electrical impedance signals of the first test sample; detecting at least two types of optical signals of the second test sample; acquiring first platelet detection data based on the electrical impedance signals; acquiring second platelet detection data based on the at least two types of optical signals; acquiring an evaluation result based on a difference between the first platelet detection data and the second platelet detection data; determining whether the evaluation result meets a preset condition to provide an alarm.
Owner:SHENZHEN MINDRAY BIO MEDICAL ELECTRONICS CO LTD

Blood cell image detection and counting method based on convolutional neural network

The invention relates to a blood cell image detection and counting method based on a convolutional neural network, and belongs to the field of medical image processing, and the method comprises the steps: dividing an obtained blood cell image data set into a training set, a verification set and a test set, and carrying out the enhancement of the blood cell image data set through employing a Mosaic algorithm; inputting the image into a CSPDarkNet53 network to obtain a plurality of feature maps, and transmitting the feature maps into a Neck network to extract fusion features; predicting the blood cell image by using an improved YOLOv4 object detection algorithm; performing confidence score sorting on the prediction frame, and obtaining a finally displayed prediction frame through a non-maximum suppression algorithm; then counting the blood cells by using the predictive tags of the cells; the prediction result of the detection model being verified again by adopting KNN and DIOU, and the problem of platelet repeated detection being eliminated. The method realizes accurate and rapid detection and counting of blood cells, and has great practical application value.
Owner:CHONGQING UNIV OF POSTS & TELECOMM

Automated microscopic cell analysis

Disclosed in one aspect is a method for performing a complete blood count (CBC) on a sample of whole blood by metering a predetermined amount of the whole blood and mixing it with a predetermined amount of diluent and stain and transferring a portion thereof to an imaging chamber of fixed dimensions and utilizing an automated microscope with digital camera and cell counting and recognition software to count every white blood cell and red blood corpuscle and platelet in the sample diluent / stain mixture to determine the number of red cells, white cells, and platelets per unit volume, and analyzing the white cells with cell recognition software to classify them.
Owner:MEDICA CORP

PLT particle detection method and device of blood cell analyzer

The invention discloses a PLT particle detection method and device for a blood cell analyzer, and the method comprises the steps: carrying out the classification of a pulse signal according to a plurality of classification precisions, obtaining a plurality of classification histograms with the classification precisions, segmenting the classification histogram with each classification precision into a preset number of reference sub-classification histograms according to a reference boundary, correcting the reference boundary in each reference sub-classification histogram to obtain an accurate sub-classification histogram, determining the number of platelet aggregation particles based on the corresponding sub-classification histograms in all the classification histograms, determining an accurate first boundary according to a preset display histogram function and the number of platelet aggregation particles, so that the platelet aggregation particles and the red blood cell particles can be distinguished more accurately in the classification histogram.
Owner:SHENZHEN COMEN MEDICAL INSTR

Improved self platelet-rich plasma preparation method

The invention relates to an improved self platelet-rich plasma preparation method. A centrifugation method is adopted two times, centrifugation is performed under the condition of 850-1875 g for 11-15minutes for the first time, and centrifugation is performed under the condition of 100 g for 12 minutes for the second time. The method is simple, the platelet recovery rate is high, the cost is low,the preparation process is closed, the pollution risk is minimized, the product quantity and platelet number can be flexibly adjusted according to clinic demands, and a new method is provided for clinic preparation of self platelet-rich plasma.
Owner:THE FIRST AFFILIATED HOSPITAL OF ARMY MEDICAL UNIV

Method for detecting biological activity of resina draconis

The invention relates to a method for detecting biological activity of resina draconis, which realizes control on whole efficacy of complex components of the resina draconis. The method comprises the following steps of: crushing the resina draconis into ethanol, carrying out ultrasonic extraction, drying the extracts, using dimethyl sulfoxide for dissolving to obtain solution, continuously diluting, and obtaining solution with geometric proportion concentration; respectively measuring blood plasma recalcification time contraction rate and platelet aggregation inhibition ratio of the resina draconis; and carrying out titer calculation according to a parallel line assay method based on quantitative responses (2.2) of appendix X IV of Chinese Pharmacopoeia II. The method is simple and easy to operate, is accurate to measure, and can be used for controlling the whole efficacy of the resina draconis with undefined active constituents.
Owner:中国医学科学院药用植物研究所云南分所

Flexible centrifuge bag and methods of use

A flexible, disposable centrifuge bag for receiving and holding a fluid sample, comprising one or more tubes and upper and lower flexible sheets having doughnut shaped configurations. The upper and lower sheets are superimposed and completely sealed together at their outer perimeters to define an outer perimeter of the centrifuge bag. The inner perimeter of the bag defines a central core. The tubes are sandwiched between the upper and lower sheets and extend from the central core of the centrifuge bag. The upper and lower sheets are sealed together at their inner perimeters such that the tubes are sealed between said upper and lower sheets at the inner perimeter. The bag may be used to harvest platelet rich plasma from whole blood in either a batch method or while simultaneously infusing additional aliquots of whole blood into the bag during centrifugation.
Owner:ARTERIOCYTE MEDICAL SYST

A reagent for stabilizing blood sample cells

The present invention disclosed a reagent that stabilizes blood sample cells, including the following concentration materials: 100 μmol / lβ-cycloped, 100 μmol / L di-sodium glycolic acid, 0.05%potassium chromate, 50 μmol / L triterfluilow,100 μmol / L Truquan, 11mmol / L citric acid, 1.5mmol / L catechine, 20 μmol / L ductoid Damo, 0.37mmol / L adenosine, 20mmol / L glucose, 1.25mmol / L?PEG.The present invention can stabilize blood cells and prevent blood plates from gathering and stabilize blood cells for at least 36 hours. The treated blood samples will not affect subsequent sample molecular analysis. In addition to being widely used in blood sample molecular analysis and testing, it is also suitable for sample cells to be used for sample cellsCells or molecular detection and analytical technology platforms with strict requirements, such as micro -current control technology, streaming cytometer, etc.
Owner:广东国盛医学科技有限公司 +1

Optical detection system, blood cell analyzer, and platelet detection method

An optical detection system, a blood cell analyzer and a platelet detection method are provided. The optical detection system includes: an optical subsystem, a flow chamber and a first detector; the optical subsystem includes a laser, a front optical assembly including an optical isolator, and a rear optical assembly including a blocking diaphragm. The laser is configured to emit a laser beam; the front optical assembly is configured to perform front optical treatment; the rear optical assembly is disposed downstream of the flow chamber in the propagation direction of the laser beam, and is configured to perform rear optical treatment on the scattered light and the laser beam converged at the blocking diaphragm; and the optical isolator is configured to isolate reflected light that is generated when the laser beam passes through the flow chamber.
Owner:SHENZHEN MINDRAY BIO MEDICAL ELECTRONICS CO LTD

Platelet-rich plasma collecting and separating device adopting physical (freezing) anticoagulation

The invention relates to the technical field of medical instruments, in particular to a platelet-rich plasma collecting and separating device adopting physical (freezing) anticoagulation, which comprises a plugging screw cap and an outer injector, an inner injector is inserted in the outer injector, a piston assembly is arranged at the bottom of the inner injector and is positioned in the outer injector. A fixed anti-rotation frame is integrally formed on the top of the piston assembly. The plasma prepared by the device and the method disclosed by the invention is pure (without exogenous anticoagulant and other additives) and has different convertible use modes: the separated plasma is in a liquid state within 30 minutes at a low temperature (above a freezing temperature), the liquid plasma can be converted into jelly-shaped gel along with temperature and time changes, different clinical requirements can be met, and the application space is expanded. By utilizing the device and the method disclosed by the invention, platelet plasma or gel with different concentrations can be prepared only by strictly mastering three elements of temperature, time and centrifugal force.
Owner:起源细胞技术(滁州)有限公司

Micro-platelet RNA sequencing detection kit based on BGISEQ-500

The invention discloses a micro-platelet RNA sequencing detection kit based on BGISEQ-500. The invention also provides a method for constructing a platelet RNA sequencing library based on BGISEQ-500.The method comprises the following steps: 1) extracting platelet RNA from a sample to be detected, and performing reverse transcription to obtain dscDNA; 2) fragmenting the dscDNA by a linker-embeddedTn5 transposase to obtain a fragmented product; 3) carrying out PCR amplification of the fragmented product to obtain an amplification product; and 4) cyclizing the amplification product to obtain the platelet RNA sequencing library. Experiments prove that below 1 ml of whole blood is adopted to construct the micro-platelet mRNA library, and an autonomously established platelet analysis procedureis used to carry out highly-sensitive and highly-specific early screening of tumors.
Owner:SHENZHEN HUADA GENE INST

Household blood sample testing device and system

PendingCN110783002ARelieve the stress of visiting a doctorMedical communicationSensorsBlood collectionDisease
The invention provides a household blood sample testing device and system, which belong to the field of medical technologies. The device includes a blood collection module, a red blood cell detectionmodule, a white blood cell detection module, a platelet detection module, a CRP detection module, a control module, a database, and a report module. The control module controls the blood collection module to perform multiple blood collections according to the number of detection modules. The blood collected each time is stored in a reagent tube. Reagent tubes after blood collection are delivered to each detection module. The database stores a normal value comparison table of blood cells and a CRP normal value comparison table. The report module forms a test report by comparison with the database based on the detection results of detection modules. The system includes a household blood sample testing device and an inquiry module arranged on a doctor side. The household blood sample testingdevice sends the disease condition and the report / detection results to the inquiry module. The inquiry module feeds back inquiry information to a user terminal. According to the invention, the deviceand system are convenient for home detection and online inquiry and used for routine detection, which saves the time of hospital visits and relieves the work pressure of doctors.
Owner:浙江艾普拉斯电子科技有限公司

Heparin-based blood sampler without platelet activation

The present invention relates to blood sampler and the preparation of blood samples that can be used for not only for blood gas, basic metabolic panel parameter analysis but also for a platelet countand / or white blood count, such as a 3-diff or 5-diff. The blood samples comprise at least one anticoagulant for the determination of blood gas and basic metabolic panel parameters and at least one anti-platelet agent.
Owner:RADIOMETER AS

Method for coagulation related function detection by EDTA (ethylene diamine tetraacetic acid) anticoagulation

The invention is a method for coagulation related function detection by EDTA (ethylene diamine tetraacetic acid) anticoagulation, and belongs to the field of medical in vitro diagnosis industry. According to the method, the EDTA anticoagulation can be used for blood cell count by a blood analyser, and can be used for detection of platelet aggregation and other blood coagulation functions.
Owner:SINNOWA MEDICAL SCI & TECH
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