44 results about "Blood count" patented technology

A method for counting blood cells in a sample of whole blood. The method comprises the steps of:(a) providing a sample of whole blood;(b) depositing the sample of whole blood onto a slide, e.g., a microscope slide;(c) employing a spreader to create a blood smear;(d) allowing the blood smear to dry on the slide;(e) measuring absorption or reflectance of light attributable to the hemoglobin in the red blood cells in the blood smear on the slide;(f) recording a magnified two-dimensional digital image of the area of analysis identified by the measurement in step (e) as being of suitable thickness for analysis; and(g) collecting, analyzing, and storing data from the magnified two-dimensional digital image.Optionally, steps of fixing and staining of blood cells on the slide can be employed in the method.

This disclosure describes the development of a sample preparation, measurement, and analysis method that permits accurate characterization of red blood cells, platelets, and white blood cells, including a 3-part differential of the white blood cells count, to be performed on small volumes of a biological sample. This method is compatible with compact and portable instrumentation that permits the sample collection to be performed in a subject's home and analysis to be performed elsewhere by transmission of the data to a laboratory or doctor's office.

Methods, systems, and computer program products for the analysis of a blood sample are disclosed. One embodiment is a method of detecting and enumerating hard-to-ghost cells in a blood sample. Another embodiments is a method of analyzing reticulocytes in a blood sample. Methods of using blood count parameters are also provided.

This disclosure describes the development of a sample preparation, measurement, and analysis method that permits accurate characterization of red blood cells, platelets, and white blood cells, including a 3-part differential of the white blood cells count, to be performed on small volumes of a biological sample. This method is compatible with compact and portable instrumentation that permits the sample collection to be performed in a subject's home and analysis to be performed elsewhere by transmission of the data to a laboratory or doctor's office.

Devices, systems, and methods are disclosed for determining the number and type of blood cells in a blood sample. The blood sample is collected and held in a slide. In the slide, the blood sample is separated and channeled into at least two sampling chambers, one for red blood cells, another for white blood cells, and optionally yet another for platelets. The sampling chambers have wetting agents, lysing agents, staining agents, or the like therein to mix with the blood and facilitate cell count. The slide is placed in a portable slide analyzer where the sampling chambers are illuminated and images of the sampling chambers are taken. These images are converted into electronic form and sent by a communications module of the slide analyzer to a remote external location where the images are analyzed to determine the number and type of blood cells in the blood sample.

A method for determining the value of MCV of a fresh sample of blood when the value of MCV for that sample of blood is known, but the period of time that the sample of blood has been stored is not known. The method of this invention allows an automated hematology analyzer to provide a more reliable indication of the initial value of MCV of a sample of blood, i.e., the value of MCV that would have been expected for the sample of blood when the sample was fresh. Furthermore, the method of this invention uses data that is readily available as part of the blood count data.

The invention relates to a passage method and an application of human induced pluripotent stem cells. The method solves the problems of easy cell differentiation, low transfection efficiency and difficult gene modification of traditional clone block passage methods. The method is a single cell passage and culture method, and comprises the following steps: fully digesting pluripotent stem cell clones to singe cells by using digestive enzyme added with a ROCK inhibitor; counting by using a blood counting chamber or a cell counter; spreading cells on an ECM coated culture plate according to a density of 20000-30000 cells / cm<2>; culturing the induced pluripotent stem cells by using a medium containing the ROCK inhibitor culture conditions comprising a temperature of 37DEG C and containing 8-15% of CO2; and passing 20 generations to obtain induced pluripotent stem cells with unchanged pluripotency including the expression of pluripotency genes, in vivo and in vitro differentiation potential and the like.

The invention discloses a determination method of the quantity of cassava pollen. The method includes steps: taking and putting 10 fully mature, plump and uncracked cassava anthers into a 2 ml centrifugal tube, and placing the centrifugal tube in a dryer in an incubator at the temperature of 30 DEG C for accelerating cracking; after completion of spilling of pollen, adding dispersing mixing liquid, and performing uniform oscillation on a vortex oscillator to form suspended pollen liquid, wherein the dispersing suspended mixing liquid is formed by 20% of sodium metaphosphate, 2% of sucrose, 2%of glucose, 0.01% of hydroxypropyl methyl cellulose, 0.04%-0.06% of xanthan gum, and the remaining water; and taking and dripping the suspended pollen liquid on a slide, and performing observation andstatistics on the particle quantity of pollen under a microscope. According to the method, the condition that the particle size of the cassava pollen is greater than 0.10 mm so that the counting cannot be performed by employing a blood counting chamber determination method for determination of the pollen quantity is overcome; besides, through selection of proper dispersing mixing liquid, the stability of the pollen suspension liquid is improved, the deposition speed of pollen is controlled, and observation is easier and counting is more accurate during determination by adopting a microscope observation method.

The present invention relates to pharmaceutical products comprising fluticasone furoate for use in the treatment of COPD patients, particularly a subgroup of COPD patients that through analysis have been identified as possessing an eosinophil blood count of≧150 cells / W. The present invention is further directed to methods for treating a patient with COPD which methods include identifying a patient that will respond to treatment and administering a pharmaceutical product of the present invention comprising fluticasone furoate to said patient.

The invention discloses a culture method of trichoderma producing cellulase. The method includes: inoculating the trichoderma onto a PDA slant, culturing at 45 DEG C for 5 d, washing spores with 5 mL of sterilized normal saline after the spores are formed, counting the collected spore solution by using a blood counting chamber so as to allow the number of the spores in each milliliter of the solution to be 106-107, inoculating onto a solid fermentation culture medium according to a 5% inoculating amount, and culturing at 28 DEG C for 146 h. The enzyme output in unit volume in trichoderma solid-state fermentation is higher than that of liquid fermentation. The method has characteristics of no stirring, low energy consumption, low equipment investment, simple post treatment and basically no pollution.

The invention discloses a cell wall broken Ganoderma Lucidum spore powder-Perilla herb oil composite softgel and a preparation technology thereof. The preparation technology comprises the following steps: 1, sieving Ganoderma Lucidum spore powder by a 20 mesh sieve, elutriating, separating, and drying at 60DEG C to obtain dried Ganoderma Lucidum spore powder for later use; 2, carrying out wall breaking of the dried Ganoderma Lucidum spore powder by adopting a roller type wall breaker, stopping the wall breaking when the wall breaking rate detected by a blood counting chamber reaches 90-97%, and carrying out sealing preservation of the cell wall broken Ganoderma Lucidum spore powder in 0-5DEG C environment to obtain cell wall broken Ganoderma Lucidum spore powder; 3, mixing the cell wall broken Ganoderma Lucidum spore powder with an oil agent composed of Perilla herb oil and vitamin E, carrying out mechanical stirring beating, carrying out colloid mill treatment to obtain a mixed slurry, and carrying out heat insulation at 50DEG C; and 4, adopting gelatin to prepare a capsule membrane, and pelletizing by using a pelletizing machine to obtain the cell wall broken Ganoderma Lucidum spore powder-Perilla herb oil composite softgel. A low temperature wall breaking process and the insulation of the softgel gelatin membrane to oxygen effectively protect the health components of the Ganoderma Lucidum spore powder and the Perilla herb oil.

The invention discloses a method for inducing grape elsinoe ampelina to produce spores. The method comprises the following steps of selecting an outer ring mycelium of a grape anthrachose pathogenic fungus colony which grows for 15 days in a culture dish with a PDA (potato dextrose agar) culture medium, and inoculating the mycelium into a new sterile culture bottle with the PDA culture medium; culturing for 25 days at the temperature of 25 DEG C under the dark environment, and putting the culture bottle under the dark environment for 24h at the temperature of 21 DEG C; using a sterile scalpel to scrape colony surface tissues, extruding into powder, putting into sterile water, oscillating for 30s, obtaining supernatant liquid, and using a blood counting chamber to count the spores. The method has the advantages that the operation is simple, more conidiums can be stably obtained, the anthrachose-resisting grape variety can be conveniently sorted, and the guarantee is provided for the disease-resistant breeding and grape pathological studying.

The invention provides a method for increasing embryoid emergence quantity in brassica chinensis isolated microspore culture. The method includes: step one, selecting a brassica chinensis bud in a late mononuclear stage, disinfecting, separating and purifying to obtain isolated microspores, suspending the purified microspores in an NLN liquid culture medium, and adjusting the microspore density to1*10<5>-2*10<5> microspores / mL by a blood counting chamber to obtain microspore suspension; step two, adding the prepared brassica chinensis microspores into a NLN-13 culture medium in which macroelements are reduced by half and 6-BA (0.05mg / L), NAA (0.10mg / L) and activated carbon (100mg / L) are added, subjecting sub-packaged microspore petri dishes to 32 DEG C heat shock treatment in a constant-temperature incubator for 24h, and then transferring into a 25 DEG C constant-temperature incubator for continuing dark culture until embryoids appear. By adoption of the method, an evident promoting effect on brassica chinensis microspore induced embryoid emergence is achieved, the microspore embryoid emergence quantity can be increased. The method has advantages of practicality, specific steps, high repeatability and the like, and extensive application of brassica chinensis isolated microspore culture to haploid breeding can be realized.

This blood component separation device: is provided with a centrifugal separator for separating a plurality of prescribed blood components from blood, and a container for holding the prescribed blood components that have been centrifugally separated; and performs a step in which the separated plurality of blood components are each collected over a plurality of cycles. The blood component separation device performs a step in which the starting point of the step for collecting the prescribed blood components is determined from blood count values of a donor on the basis of map data pertaining to pre-stored blood count values or the concentrations of prescribed blood components, in such a manner that the concentrations of the prescribed blood components become equal at each point that the blood components are discharged from the centrifugal separator at the collection starting point and finishing point in the step for collecting the prescribed blood components.

A blood test device is configured to measure a blood count and an electrolyte in a blood sample. The blood test device includes: a sample placement section in which a blood sample containing an anticoagulant containing a salt of ethylenediaminetetraacetic acid (EDTA) other than sodium salt, potassium salt, or calcium salt of EDTA is placed; and a measurement section by which measurement of a blood count and an electrolyte is carried out for the blood sample placed in the sample placement section.

The invention relates to the technical field of blood volume measuring equipment, in particular a countable drainage bag, comprising a blood drainage bag, wherein the blood drainage bag includes a bagbody, a blood counting calibration area is arranged on the outer surface of the bag body, a drainage structure is arranged at the center of the bag body, a drainage structure includes a drainage tube, the top of the drainage tube is connected with a finite flow tube, the diameter of the current limiting tube from top to bottom increases from small to large, an end portion of the flow restrictiontube remote from the drainage tube passes through the top surface of the bag body and extends to the outside of the bag body, one end part of the flow restriction tube away from the flow restriction tube is connected with a blood drawing connecting tube, the outer wall of the flow restriction tube is provided with uniformly equidistant arranged through holes, one end part of the flow restriction tube away from the flow restriction tube is connected with a sealable tube, a mesh cover is installed at the bottom of the flow restriction tube, and a floating ball is placed inside the flow restriction tube. The invention can read out the blood volume in the bag through the blood counting calibration area arranged on the bag body, and the recording panel can assist the medical personnel to recordthe blood volume.

The invention relates to a method for performing an experiment for observing molecular motion, which comprises the following steps: grinding, during which water is filled in a vessel and grinding an ink stick in the vessel; liquid taking, during which 1 to 3 drops of liquid is taken by a burette after the ink stick is ground in the vessel for a plurality of times and placed into a groove on a blood counting chamber, cover glass is covered, and excessive water on the edge is absorbed by paper; and the placement of the blood counting chamber on a viewing platform of a microscope and the adjustment of the microscope for observation of 'molecular motion trace' represented by a plurality of carbon particles from an ocular lens. A camera on a video display stand can be aimed at the ocular lens of the microscope vertically, and the focus of the video display stand is adjusted till 'molecular random motion' represented by a plurality of carbon particles can be seen on the large screen. According to the technical proposal, the 'molecular motion trace' can be observed with a low power biological microscope in a common middle school by using ink produced by grinding an ink stick in the vessel, single-student observation can be changed into the real-time common observation of the students in class by the conversion and amplification of the video display stand, and a teacher can give real-time analysis and explanation. Thus, the experiment is more visual and universal, the interests of students are aroused, and the classroom teaching effect is improved.

The invention relates to a method for finding early bacterium infection of fermentation liquor. The method comprises the following steps of preparing culture mediums, performing inoculation into a strain to be fermented, and beginning fermentation; performing fermentation for some time, and before the fermentation liquor reaches a given OD value, taking out fermentation liquor samples; calculatingthe bacterium concentration of the taken-out fermentation liquor samples with a blood cell counting chamber, and according to the bacterium concentration of the fermentation liquor samples, determining the multiple to be concentrated of the fermentation liquor samples, wherein the concentration multiple is large enough to concentrate the bacterium concentration of the sampled fermentation liquorsamples to the concentration of given microscope inspection bacteria which are suitable for observation under a microscope; according to the determined concentration multiple, concentrating the fermentation liquor samples to the concentration of the microscope inspection bacteria; observing the concentrated samples having given area under the microscope, and determining whether infectious microbesexist or not and the number of the infectious microbes; and based on the existence and the number of the infectious microbes contained in the concentrated samples having the given area, judging wither the fermentation liquor has a phenomenon of bacterium infection or not.

The invention discloses a blood cell pulse counting error correction method. Determine whether the reference pulse signal is valid; extract the valid reference pulse signal, calibrate the gem hole error, and calculate the calibration coefficient; collect the actual pulse signal of blood cells passing through the gem hole, and extract the pulse waveform of the actual pulse signal; use the calibration coefficient to re-fit The falling edge pulse waveform of the actual pulse signal. The method first collects the pulse waveform of the standard particle, and then calculates the correction coefficient of the gem hole by analyzing the pulse waveform of the standard particle, and uses the correction coefficient to re-fit the actually collected blood cell pulse waveform, so as to accurately measure the pulse amplitude data. The correction greatly improves the accuracy and reliability of blood counts.

The invention relates to a method for evaluating the fungus killing effect of a liquid disinfectant. The evaluation steps include: sample extraction and determination of the minimum effective concentration; The live bacteria content of bacterial suspension after staining C B Blood count and inoculum C B Calibration; lgC B ‑ Relative fluorescence intensity log value lgI B Standard song establishment; neutralizer identification and quantitative suspension test; recovery solution I B Determination and its live bacteria content C B and T B Calculation; Calculation of killing rate R and sterilization index A; Judgment of results; The special feature is: the use of ATP fluorescence photometer to accurately and quantitatively evaluate the fungal killing effect of disinfectants characterized by R or A. The present invention stipulates to reclaim the test sample liquid and the control sample liquid after the different time of action, measure its I B and with lgI B Characterize and calculate R or A; provide the basis for judging the results. ATP bioluminescent IgC for the evaluation of the fungal killing effect of the disinfectant developed by the present invention B ‑lgI B The standard music method can improve the relevant evaluation method system of disinfection effect.

A device for full blood count includes first channel and second channels separated from each other. The device further includes a first inlet configured to provide a whole blood sample to the first and second channels, a second inlet configured to provide a lysis agent for white blood cell count in to the first channel, a third inlet configured to provide a quench solution to the first channel, and a fourth inlet configured to provide a lysis agent for hemoglobin measurement to the second channel.

This blood component separation device: is provided with a centrifugal separator for separating a plurality of prescribed blood components from blood, and a container for holding the prescribed blood components that have been centrifugally separated; and performs a step in which the separated plurality of blood components are each collected over a plurality of cycles. The blood component separation device performs a step in which the starting point of the step for collecting the prescribed blood components is determined from blood count values of a donor on the basis of map data pertaining to pre-stored blood count values or the concentrations of prescribed blood components, in such a manner that the concentrations of the prescribed blood components become equal at each point that the blood components are discharged from the centrifugal separator at the collection starting point and finishing point in the step for collecting the prescribed blood components.

The invention discloses a method for separating peripheral blood mononuclear cells from a repayment-free blood donation whole blood collection device, which comprises the following steps: clamping two side tube walls by using a tube clamp under a sterile condition, cutting open upper and lower end tube orifices of the two side tube walls by using sterile scissors, and reserving a section of long tube on each of the two side tube walls; using an injector to forward flush the white filter and backward flush the white filter with the flushing fluid, collecting the liquid obtained by backward flushing, centrifuging, sucking and discarding the supernatant after centrifuging, re-suspending cells with normal saline, adding into another two centrifugal tubes filled with the same amount of Ficoll, and continuing centrifuging; and after centrifugation is finished, sucking and abandoning supernatant, sucking the albuginea layer above the Ficoll into a new centrifuge tube, supplementing normal saline, sucking and abandoning supernatant after centrifugation, and manually counting by adopting a blood cell counting plate after the normal saline is resuspended. According to the method disclosed by the invention, the mononuclear cells can be aseptically extracted from the white filter of a blood donation and collection pipeline and cultured in vitro, and the cells cultured in vitro are used as DC-CIK treatment cells.

The invention discloses a detecting method for suspension yeast concentration and relates to the technical field of beer brewing technology. The detecting method comprises the following steps: preparing a sample: taking a preset volume of to-be-detected sample liquor, adding a regulator which contains sugars capable of being decomposed and utilized by yeast, uniformly mixing and regulating to thepreset volume, thereby acquiring the to-be-detected liquor; counting: taking the to-be-detected liquor, injecting into a blood counting chamber and counting microscopically. According to the method, sugars, such as wheat juice, compound syrup and high fructose corn syrup, capable of being decomposed and utilized by yeast are added into the to-be-detected sample liquor (yeast solution), so that thecontent of sugars capable of being utilized by yeast solution is increased, the dispersion of yeast in solution is boosted, the dispersion is high in uniformity, the subsequent microscopic counting is benefited and the accuracy of counting result is guaranteed.

The invention discloses a blood counting chamber, and belongs to the field of biological equipment. The blood counting chamber comprises a bottom plate and cover glass. Counting pools are arranged on the upper surface of the bottom plate; liquid isolating pieces capable of moving on the upper surface of the bottom plate are further arranged on the bottom plate. According to the scheme, the blood counting chamber has the advantages that redundant sample liquid in the counting pools can be pushed out of the counting pools by the liquid isolating pieces when the blood counting chamber is used, accordingly, the actual volume dose of sample liquid in the counting pools is consistent with the volumes of the counting pools, the problem that the actual volume of sample liquid in existing counting pools is larger than or smaller than the volumes of the existing counting pools can be solved, and the liquid sample counting precision can be improved.

The invention relates to the technical field of biological effects of negative ion health-care functional textiles, and particularly discloses a method for testing influence of negative ion functional textiles on oxygen carrying capacity of red blood cells in animals, the method is as follows: 1, preparing a negative ion functional textile material and selecting an animal as a test object; 2, putting the test object of the step 1 into a bag prepared from the negative ion functional textile material, tying the bag tightly, continuously placing the test object in a space left in the bag for moving of the test object for 3-5h, and then taking the test object out; 3, putting the test object taken out in the step 2 in water at temperature of 25-35 DEG C for swimming for 7-12 minutes, then immediately picking eyeballs of the test object off, collecting blood flowing out, separating blood serum from the collected blood, and using a globulimeter to test red blood count, hematokrit and hematocrystallin numerical value; simulation ability is good, precision is high, and product improvement of the negative ion functional textiles can be facilitated.