35results about How to "Determination of precision" patented technology

The invention relates to a method for measuring content of 5-fluorouracil in plasma and colorectal cancer cells based on high performance liquid chromatography and belongs to the technical field of liquid chromatography medical analysis. 5-fluorouracil (5-Fu) is a miazines antineoplastic drug widely applied clinically at present; the antineoplastic chemotherapy effect of 5-Fu is related to the concentration of 5-Fu in the cancer cells; other side effects are caused when the drug concentration in the body is ultrahigh. For the purpose of enhancing the drug therapeutic effect and reducing the side effects, the trend of the concentration of 5-Fu in the cancer cells and the drug concentration in human body shall be strictly monitored. The monitoring has significant clinical significance in promotion of drug therapeutic effect, reduction of toxicity and prevention of post-operation transfer. According to the method provided by the invention, 5-Fu in plasma and colorectal cancer cells is extracted in the manner of solid phase extraction, so that the extraction efficiency is increased; the chromatographic condition is optimized; the peak pattern is improved by adding a trifluoroacetic acid and methyl alcohol system into a detected flow phase; the number of theoretical plates is increased and the chromatographic condition is mild; under a selected optimal chromatographic condition, the measurement for 5-Fu is more precise and accurate than the measurement in literature reports.

The present invention discloses a labor analgesia method and an analgesia device. The analgesia method is as follows: firstly, pregnant women are injected with analgesic, then body temperatures of the pregnant women are scanned, skin temperature changes of the pregnant women after analgesia are detected, an anesthesia plane is confirmed, and then according to differences between an actual anesthesia plane and an ideal anesthesia plane, analgesic dose is added until the analgesia is completed. The analgesia device comprises a skin temperature detecting probe, a PLC controller and an analgesia pump, the skin temperature detecting probe is in a communication connection with the PLC controller, and the analgesia pump is electrically connected with the PLC controller. The used device and method can effectively solve technical problems of labor difficulties of the pregnant women.

The invention discloses a nonaqueous titration method for determining the acidity of glyceryl triacetate. The method is characterized in that: waterless ethanol is used to dilute glyceryl triacetate as a diluent, a KOH standard solution is used as a titrant, an automatic potentiometric titration process is adopted to titrate, the end point is automatically determined by a titrator, and the acidity (by acetate) is obtained through calculating by the titrator. The method for rapidly and precisely determining the acidity of glyceryl triacetate, which is provided in the invention and can be used to control the quality of glyceryl triacetate, provides a technical support for the practical production of glyceryl triacetate.

The invention discloses a cell periodic detection kit. The cell periodic detection kit comprises a reagent I and a reagent II; the reagent I comprises a DMEM culture medium, 0.05 to 0.5 percent trypsin and cleaning buffering solution; and the reagent II comprises 2 to 5 ml of Cystain DNA1 step, 100 to 120 micro liter of low-permeability cracking buffering solution, 0.5 to 5 micro liter of phosphoric acid histochemical protein H3 primary antibody and 0.2 to 2.0 micro liter of phosphoric acid histochemical protein H3 secondary antibody. The kit utilizes flow cytometry technology, so that the cell period can be rapidly and accurately determined, and the period G2 and the period M can be distinguished.

The invention relates to the technical field of a method for determining the preservation state of water-saturated wooden cultural relics, and provides a rapid non-destructive testing method for the preservation state of water-saturated wooden cultural relics. The water-saturated wooden cultural relics are tested; the data acquisition step is to obtain the spectral data after the spectrometer test, and the spectral data is processed; the establishment of a calibration model step is used to establish a calibration model for the preservation state of the water-saturated wooden cultural relics; the preservation state step is determined, It is used to test the spectral data and clustering behavior of water-saturated wooden artifacts through the aforementioned steps, and determine their preservation status. With this technical solution, after the testing step and the data acquisition step, the steps of establishing a calibration model and determining the preservation state can be used to accurately determine the preservation state of the water-saturated wooden cultural relics, and it has the advantages of no destructive sampling damage and fast test speed. The advantages.

The invention relates to a method for evaluating the fungus killing effect of a liquid disinfectant. The evaluation steps include: sample extraction and determination of the minimum effective concentration; The live bacteria content of bacterial suspension after staining C B Blood count and inoculum C B Calibration; lgC B ‑ Relative fluorescence intensity log value lgI B Standard song establishment; neutralizer identification and quantitative suspension test; recovery solution I B Determination and its live bacteria content C B and T B Calculation; Calculation of killing rate R and sterilization index A; Judgment of results; The special feature is: the use of ATP fluorescence photometer to accurately and quantitatively evaluate the fungal killing effect of disinfectants characterized by R or A. The present invention stipulates to reclaim the test sample liquid and the control sample liquid after the different time of action, measure its I B and with lgI B Characterize and calculate R or A; provide the basis for judging the results. ATP bioluminescent IgC for the evaluation of the fungal killing effect of the disinfectant developed by the present invention B ‑lgI B The standard music method can improve the relevant evaluation method system of disinfection effect.

The invention relates to a method for evaluating the fungus destroying effects of daily chemical products. The method comprises the detection steps of meeting requirements of sample extraction and pretreatment; performing type selection of equipment, and compounding a reagent and a culture medium; performing strain preservation, performing strain activation and preparing fungus suspension; after methylthioninium chloride staining, counting the content C of viable fungi in the fungus suspension through a hemocytometer; establishing a standard curve of ATP concentration log lgC-relative fluorescence intensity log lgI, and performing calibration on C of viable fungi in inoculated fungus liquid; performing neutralizer identification and performing fungus destroying experiment; determining I of recovered liquid, and reckoning C and T of the recovered liquid; calculating fungus destroying rate R and fungus destroying index A; and performing result evaluation. The method has the characteristic that the fungus destroying effects of the daily chemical products, which are expressed by R or A, can be accurately and quantitatively evaluated through an ATP fluorophotometer. Control samples and experiment samples after special time of fungus infection are recovered according to the specification of the invention, the I of the recovered liquid of the control samples and the I of the recovered liquid of the experiment samples are determined and expressed by lgI, and R or A is calculated; and a result evaluation base is provided. According to the ATP biofluorescence lgC-lgI standard curve method for detecting the fungus destroying properties of the daily chemical products, researched and developed by the invention, product quality increase is supported through an advanced detection technique.

The invention relates to a method for detecting anti-mildew property of an antibacterial wood board, and the detecting steps include: sample preparation and pre-treatment; equipment selection, and reagent and medium preparation; species preservation and activation, and preparation of spore liquid; establishment of OD value-live bacteria content logarithmic value lgCB standard curve; establishmentof ATP concentration logarithmic value lgCA-relative fluorescence intensity logarithm lgIA standard curve and inoculated bacterial liquid live bacteria CA calibration; sample inoculation, culture andelution recovery; IA determination of recovered liquid and its live bacteria ATP concentration CA and TA reckoning; antibacterial rate R or antibacterial activity value A calculation; and results evaluation. The method is characterized in that an ATP fluorescence photometer is used to accurately and quantitatively test the mildew resistance of the wood board characterized by R or A. The method prescribes the elution and recovery of a control sample and an antibacterial sample after contact for 0 h and culture for 48 h, and the recovered liquid IA is determined and R or A is characterized and calculated by lgIA. The ATP bio-fluorescence lgCA-lgIA standard curve method supports the product quality improvement by a scientific and advanced detection technology.