Method for detecting anti-mildew performance of an antibacterial coating by ATP biological fluorescence lgC-lgI standard curve method

Abstract

The invention relates to a method for detecting the anti-mildew performance of an antibacterial coating. The method comprises the following detection steps: sample extraction, preparation and pretreatment; equipment selection, and reagent and culture medium preparation; strain preservation and activation, and spore suspension preparation; establishment of a standard curve of OD value-viable content log value lgC; establishment of a standard curve of ATP concentration log value lgC-relative fluorescence intensity log value lgI, and calibration of living bacteria C of an inoculation bacterial liquid; sample inoculating and culturing, and eluting recovery; determination of recovery liquid I, and calculation of living bacteria ATP concentration C and T; calculation of antibacterial rate R or the antibacterial activity value A; and result evaluation. The method is characterized in that: an ATP fluorophotometer is used for accurately and quantitatively testing the anti-mildew performance of a coating characterized by R or A. The method comprises the following steps: eluting and recovering a control sample and an antibacterial sample after contacting for 0h and culturing for 48h, determining recovery liquid I, and characterizing and calculating R or A by the lgI. The ATP biological fluorescence lgC-lgI standard curve method for detecting the anti-mildew performance of an antibacterial coating developed by the invention can support the product quality improvement by using an advanced detection technology.

Description

technical field [0001] The invention relates to a method for testing the mildew resistance of antibacterial coatings, in particular to an ATP bioluminescent IgC that uses an ATP fluorescence photometer to accurately and quantitatively detect the mildew resistance of antibacterial coatings characterized by antibacterial rate R or antibacterial activity value A A -lgI A The standard curve method belongs to the technical field of paint antibacterial function detection. Background technique [0002] With the improvement of people's living standards and the enhancement of health awareness, the indoor environment and the quality of home decoration have attracted much attention, and antibacterial coatings have emerged as the times require and the supply exceeds demand; according to statistics, the global production of antibacterial coatings in 2017 has exceeded 400,000 tons; related latest research reports It shows that the international antibacterial coating market is expected to...

AI Technical Summary

Problems solved by technology

In general, the existing anti-mold performance testing methods have the following common problems in technical content and practical application: First, because the mycelium formed during the growth of mold spores cannot be accurately counted, only qualitative judgments can be made, and quantitative tests cannot be performed; The second is that the mold growth cycle is long, and the cultivation time is at least 2 to 4 weeks, resulting in a long test cycle and high time and economic costs; the third is that the experimental process is cumbersome and technically difficult; related operations are greatly affected by human factors, making Large test error, lack of comparability

Driven by the demand, foreign antibacterial material performance testing technology research field has developed ISO 20743:2007-2013 "Textiles-Determination of antibacterial activity of textile products (textiles- Determination of Antibacterial Performance of Antibacterial Finished Textiles) and ISO 13629-1:2012 "Textiles-Determination of antifungal activity oftextile products. Fluorometric assay of anti-microbial / mold performance by content change, but it is only applicable to textile or microporous materials with water absorption and control sample / mycotic growth value > 0. Aspects are not applicable to metal, wood and other painted floor materials with non-absorbent hard surfaces and mold growth value of the control sample <0, and no evaluation of measurement uncertainty is provided

In addition, the relevant antibacterial performance characterization parameters of this standard method are relatively single, only involving the antibacterial activity value A, while the antibacterial rate R commonly used in my country

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