Method for evaluating fungus destroying effect of liquid disinfecting agent by ATP biofluorescence lgC-lgI standard curve method

Abstract

The invention relates to a method for evaluating the fungus destroying effect of a liquid disinfecting agent. The method comprises the detection steps of performing sample extraction and determining the lowest effective concentration of a disinfecting agent; performing type selection of equipment and preparing a reagent and a culture medium; performing strain preserving and activating and preparing fungus suspension; after methylthioninium chloride staining, counting the content C of viable fungi in the fungus suspension through a hemocytometer; establishing a standard curve of the ATP concentration log lgC-relative fluorescence intensity log lgI and performing calibration on C of viable fungi in inoculated fungus liquid; performing neutralizer identification and quantitative suspension test; performing I determination and C and T reckoning on recovered liquid; calculating destroying rate R and fungus destroying index A; and performing result judgment. The method has the characteristics that the fungus destroying effect of the disinfecting agent, expressed by R or A, is subjected to pinpoint quantitative evaluation through an ATP fluorophotometer. The I of test sample liquid and the I of control sample liquid after different time of action are regulated and determined and expressed with lgI, R or A is calculated, and a result judgment basis is provided. The ATP biofluorescence lgC-lgI standard curve method for evaluating the fungus destroying effect of the liquid disinfecting agent, which is researched and developed, can perfect a relevant evaluation method system.

Description

technical field [0001] The invention relates to a method for evaluating the fungicidal effect of a liquid disinfectant, in particular to an ATP method for accurately quantitatively testing the fungicidal effect of a liquid disinfectant characterized by a killing rate R or a sterilization index A using an ATP fluorescence photometer. bioluminescent lgC A -lgI A The standard curve method belongs to the technical field of disinfectant sterilization effect evaluation. Background technique [0002] With the improvement of people's living standards and the enhancement of health awareness, in order to cut off the transmission of infectious diseases, liquid disinfectants such as peroxides, aldehydes, phenols, and quaternary ammonium salts are widely used in medical treatment, food, education, catering, and home use. The field of application is becoming more and more extensive, and the killing effect and broad-spectrum requirements for pathogenic microorganisms such as bacteria, fu...

AI Technical Summary

Problems solved by technology

Driven by the demand, foreign antibacterial material performance testing technology research field has developed ISO 20743:2007-2013 "Textiles-Determination of antibacterial activity of textile products (textiles-determination of antibacterial activity - Determination of antibacterial performance of antibacterial finishing textiles)" and ISO 13629-1:2012 "Textiles-Determination of antifungal activity of textile products. The change of ATP content characterizes the anti-microbial / fungal performance of the fluorescence assay, but the absorption method, transfer method and transfer method provided by it are only suitable for textile materials or microporous materials with water absorption and control sample / mycotic growth value > 0 ; The evaluation of the fungicidal effect of liquid disinfectants must form a set of clear and specific methods for key technical aspects such as neutralization effect evaluation and result calculation formulas, and relevant measurement uncertainty evaluations must be provided

In addition, the antibacterial performance characterization parameters of this standard method are relatively single, only involving the antibacterial activity value A, while the traditional killing rate R in my country is

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