The invention discloses a method for simultaneous in-situ determination of enzymatic activity between
straw and
rhizosphere. The method comprises the following steps of: loading soil and
straw into a root box, wherein the root box is wrapped with a light-proof material and obliquely placed on a support;
transplanting plant seeds into the root box after
germination, and after plants grow, exposing the plants out of soil surfaces and
plant roots; meanwhile, taking a microporous filter membrane soaked in a substrate solution marked by fluorescent
pigment, covering the surface of the microporous filter membrane, wrapping the root box with the light-proof material, and carrying out light-proof culture; after the culture is finished, taking down the microporous filter membrane, transferring the microporous filter membrane into an
ultraviolet lamp box, and performing imaging under a 355nm reflection
ultraviolet lamp; and
processing and analyzing the obtained image, and comparing the image with a
standard curve to obtain two-dimensional distribution of the enzymatic activity between the
straw and the
rhizosphere on the surface of the soil, namely simultaneously obtaining the enzymatic activity between the straw and the
rhizosphere. According to the method, the in-situ
enzyme activity determination between straw and rhizosphere under the control condition can be realized,
destructive sampling of soil and plants is avoided, and the method is convenient and quick.