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Research method of soil microbial diversity and plant stress interaction

A technology of soil microorganisms and diversity, applied in the field of microorganisms, can solve problems such as time-consuming, cumbersome operations, and inability to create physical and chemical conditions, and achieve the effect of simple operation and short time-consuming

Active Publication Date: 2022-04-22
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The above methods also cannot rule out the interference of microbial populations on the interaction between soil microbial diversity and plant adversity, that is to say, under the same microbial population conditions, it is impossible to clarify the function of soil microbial diversity in assisting plants to resist abiotic stress
In addition to field investigations, some studies have studied the interaction between microorganisms and plants by directly inoculating microbial strains on plants, which cannot create the same physical and chemical conditions as in-situ soils
Moreover, the method of soil collection and index monitoring based on field experiments takes a long time, more than half a year, and it is impossible to quickly and visually observe the apparent phenomenon of the interaction between soil microbial diversity and plant adversity in situ, and can only be carried out through destructive sampling. Observation, resulting in cumbersome operation

Method used

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  • Research method of soil microbial diversity and plant stress interaction
  • Research method of soil microbial diversity and plant stress interaction
  • Research method of soil microbial diversity and plant stress interaction

Examples

Experimental program
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Effect test

Embodiment 1

[0048] A visual research method for the interaction between soil microbial diversity and plant adversity, the steps are as follows:

[0049] (1) Select forest soil that has not used chemical fertilizers and pesticides, use a 10cm diameter, 30cm depth sterilized soil drill to collect surface forest soil, the collection depth is 0-30cm, and a total of 20 points are randomly selected, and the soil samples are placed in a ziplock bag Then put the ziplock bag into an insulated box with ice packs;

[0050] (2) Sieve the soil sample collected in step (1) with a sterilizing sieve with an aperture of 2mm, and then use a sterile cloth bag or plastic bag to fully mix all the soil to obtain the in situ soil;

[0051] (3) Sterilize the original soil soil under gamma rays of 50kGy intensity to obtain sterile soil;

[0052] (4) Take 200g of native soil and put it into a sterile beaker, add 500mL of sterile deionized water, and use a sterile glass rod to fully stir it evenly. 10 0 、10 2 、...

Embodiment 2

[0058] A visual research method for the interaction between soil microbial diversity and plant adversity, the steps are as follows:

[0059] (1) Select forest soil that has not used chemical fertilizers and pesticides, use a 10cm diameter, 30cm depth sterilized soil drill to collect surface forest soil, the collection depth is 0-30cm, and a total of 20 points are randomly selected, and the soil samples are placed in a ziplock bag Then put the ziplock bag into an insulated box with ice packs;

[0060] (2) Sieve the soil sample collected in step (1) with a sterilizing sieve with an aperture of 1.5 mm, and then use a sterile cloth bag or plastic bag to fully mix all the soil to obtain the in situ soil;

[0061] (3) Sterilize the original soil soil under gamma rays of 40kGy intensity to obtain sterile soil;

[0062] (4) Take 300g of native soil and put it into a sterile beaker, add 500mL of sterile deionized water, and use a sterile glass rod to fully stir it evenly. 10 0 、10 ...

Embodiment 3

[0067] A visual research method for the interaction between soil microbial diversity and plant adversity, the steps are as follows:

[0068] (1) Select forest soil that has not used chemical fertilizers and pesticides, use a 10cm diameter, 30cm depth sterilized soil drill to collect surface forest soil, the collection depth is 0-30cm, and a total of 20 points are randomly selected, and the soil samples are placed in a ziplock bag Then put the ziplock bag into an insulated box with ice packs;

[0069] (2) Sieve the soil sample collected in step (1) with a sterilizing sieve with an aperture of 2.5 mm, and then use a sterile cloth bag or plastic bag to fully mix all the soil to obtain the in situ soil;

[0070] (3) Sterilize the original soil soil under gamma rays of 60kGy intensity to obtain sterile soil;

[0071] (4) Take 100g of native soil and put it into a sterile beaker, add 500mL of sterile deionized water, and use a sterile glass rod to fully stir it evenly. 10 0 、10 ...

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Abstract

The invention provides a visual research method for interaction of soil microbial diversity and plant stress, and belongs to the technical field of microorganisms, the method comprises the following steps: sterilizing original habitat soil, respectively mixing original habitat soil gradient diluents with sterile soil, and culturing in darkness to obtain a microbial diversity gradient soil planting plate; through the modes of direct sowing, stress treatment after direct sowing and the like, the function of soil microbial diversity in plant stress is determined under the condition of the same microbial quantity, and regulation and control of the microbial diversity on plant growth under the stress are accurately reflected. The research method disclosed by the invention is short in time consumption, only needs 45-60 days, can be used for visually observing the response of the root system to the microbial diversity under the adverse situation in situ, does not need destructive sampling observation, and is simpler, more convenient and quicker to operate.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a research method for the interaction between soil microbial diversity and plant adversity. Background technique [0002] The number and variety of microorganisms in the soil are huge, and the microbial diversity of the soil affects the structure, soil fertility, and soil nutrient utilization rate of the soil, which in turn affects the growth and development of plants. At the same time, plants are also a key determinant of soil microbial diversity. In recent years, there have been more and more studies on the interaction between plants and soil microorganisms at home and abroad, but there are few reports on the research methods of the interaction between soil microbial diversity and plant adversity. [0003] At present, research usually adopts the method of field test soil collection and indicator monitoring, and through the correlation analysis between microbial diversity...

Claims

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Application Information

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IPC IPC(8): A01G7/00C12M1/34
CPCA01G7/00C12M41/46
Inventor 田永强李佳璠高丽红剌世凯宋梦圆
Owner CHINA AGRI UNIV
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