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A kind of non-destructive sampling method and application of live crayfish

A non-destructive sampling technology of Crayfish, applied in the fields of application, biochemical equipment and methods, climate change adaptation, etc., can solve the problems of sampling target disability, decline in vitality, etc., and achieve the effect of easy collection

Active Publication Date: 2021-12-21
FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
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AI Technical Summary

Problems solved by technology

[0003] When sampling economic crustacean breeding parents for molecular detection, the method of amputating limbs and taking muscle is usually used. This method causes the sampling target to be disabled, the vitality is reduced, and some individuals die due to infection. Therefore, this method is not suitable for shrimp and crab molecules. linked marker assisted breeding

Method used

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Experimental program
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Effect test

Embodiment 1

[0030] 1. In October 2016, 2,000 ovum-bearing parents of Procambarus clarkii were collected from a farm in Yandu District, Jiangsu Province. The individuals were healthy, growing well, with complete appendages. Use a tightenable plastic collar with a numbered number to buckle at the base of the claws of the Procambarus clarkii to number each brooding shrimp;

[0031] 2. For each marked individual, use clean and disinfected tweezers to collect 5-10 fertilized eggs of different developmental stages (a total of 15-30 eggs) in the front, middle and back three parts of the egg-bearing shrimp abdomen, and combine them in Quick-frozen in liquid nitrogen in an Eppendorf tube and brought back to the laboratory for temporary storage in a -80°C refrigerator;

[0032] 3. Genomic DNA was extracted from the fertilized eggs of Procambarus clarkii 2000 at different development stages, and the extraction method was Soil Genomic DNA Kit from Beijing Kangwei Century Bioreagent Co., Ltd.

[0033...

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Abstract

The invention discloses a live non-destructive sampling method of Procambarus clarkii and its application. The fertilized eggs of the ovipositing parents of the Procambarus clarkii are sampled to obtain sampling samples. Through the detection of molecular markers linked to specific economic traits, screen the brood parents containing the molecular markers linked to the target traits, carry out isolation and breeding, and artificially reproduce to obtain the first-generation population of Prochela Klinefelter, and continue to screen the population for the next generation of linkage markers. A breeding population in which the frequency of genes linked to markers for target traits can reach 100%. The present invention solves the problem of non-destructive sampling in the molecular linkage marker-assisted breeding of Procambarus clarkii, provides a scientific and feasible way for the molecular marker-assisted breeding of Procambarus clarkii, and is expected to breed Procambarus clarkii with excellent characteristics. A new variety of shrimp can improve the economic benefits of Procambarus clarkii farming.

Description

technical field [0001] The invention relates to molecular marker assisted breeding technology of economic crustaceans, in particular to a method and application of live non-destructive sampling of shrimp and crabs with ovipositing habit. Background technique [0002] The growth of economic crustaceans is carried out through molting. Procambarus clarkii molts (shells) more than 20 times in a lifetime. It is difficult to use physical surface marking methods similar to fish in crustaceans. Family selection is in most It is difficult or even impossible to carry out economic crustaceans. At present, the breeding of economic crustaceans mainly adopts the method of group selection breeding. Traditional selective breeding has advantages in growth traits, but the efficiency of selective breeding is very low for traits such as reproduction and disease resistance due to low heritability. For traits with low heritability such as reproduction and disease resistance, marker-assisted bree...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888A01K61/59A01K61/17
CPCA01K61/17A01K61/59C12Q1/6888C12Q2600/156Y02A40/81
Inventor 沈怀舜黄鹏丹胡亚成
Owner FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
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