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Molecular assays for rapid identification of eucalyptus populations

A technology for light-skinned wood and molecular detection, which is applied in the field of molecular biology to achieve the effects of high discrimination accuracy, high efficiency and good stability

Active Publication Date: 2020-12-11
GUANGDONG ACAD OF FORESTRY
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0006] At present, there are no relevant reports on the molecular rapid identification of the population of P. glabrata at home and abroad

Method used

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  • Molecular assays for rapid identification of eucalyptus populations
  • Molecular assays for rapid identification of eucalyptus populations
  • Molecular assays for rapid identification of eucalyptus populations

Examples

Experimental program
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Embodiment 1

[0027] Example 1 A kind of molecular detection method for rapid identification of eucalyptus populations

[0028] see figure 1 , is a flow chart of a molecular detection method for rapid identification of eucalyptus populations. The detection method includes the following steps:

[0029] (1), according to the routine method in this field, carry out DNA extraction to the leaf sample that comes from 2 populations of Jiangxi and Guangdong altogether 20 eucalyptus plants;

[0030] (2), respectively using the DNA extracted in step (1) as a template, respectively using SEQ ID No: 1-2, SEQ ID No: 3-4, SEQ ID No: 5-6, SEQ ID No: 7-8, SEQ ID No: 9-10, SEQ ID No: 11-12, SEQ ID No: 13-14, SEQ ID No: 15-16, SEQ ID No: 17-18, SEQ ID No: 19-20, SEQ ID Nos: 21-22, SEQ ID Nos: 23-24, SEQ ID Nos: 25-26, SEQ ID Nos: 27-28, and SEQ ID Nos: 29-30 were used as primer pairs for PCR amplification respectively to obtain microsatellites molecular marker data;

[0031] Wherein, the sequence of the ...

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Abstract

The invention discloses a molecular detection method for rapidly identifying cornus wilsoniana population. The method comprises the following steps: respectively carrying out PCR amplification by using DNA of cornus wilsoniana as a template and taking SEQ ID No: 1-30 as primer pairs to obtain microsatellite molecular marker data; and carrying out statistic analysis on the microsatellite molecular marker data to identify and classify the cornus wilsoniana population. According to the molecular detection method for rapidly identifying cornus wilsoniana population, genome information can be rapidly obtained on the premise of non-destructive sampling, the work load is small, the efficiency is high, meanwhile, discrimination accuracy reaches up to 95%, and the method has the advantages of accuracy, time saving and labor saving. The method has important value and application prospect on rapid molecular detection of species population.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and more specifically, the invention relates to a molecular detection method for rapidly identifying populations of eucalyptus. Background technique [0002] Cornus wilsoniana Wanger, also known as cornus wilsoniana Wanger, is also known as cornus wilsoniana Wanger, oil tree, dogbone wood, variegated bark pumping tree, etc. It has been used as edible oil for 100 years. In 1981, it was appraised by the Ministry of Food and Agriculture as a first-class edible oil. In December 2013, the National Health and Family Planning Commission approved the Fruit oil is a new food raw material in my country. The results of animal and clinical trials show that the balsa wood oil can significantly reduce cholesterol in the body, prevent and treat hyperlipidemia, especially hyperlipidemia in the elderly, and has the effect of losing weight. Bark bark is an important oil tree species with economic, ecolo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/686C12Q1/6895C12Q2600/156C12Q2525/151
Inventor 王明怀林军黄金华张良波张应中徐煲铧张绍勋邝兆勇邓文剑叶代全何祯祥
Owner GUANGDONG ACAD OF FORESTRY
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