Method for detecting mold resisting properties of electrical household appliances by ATP biofluorescence lgC-lgI standard curve method

Abstract

The invention relates to a method for detecting the mold resisting properties of electrical household appliances. The method comprises the detection steps of preparing samples and performing pretreatment: performing type selection of equipment, and compounding a reagent and a culture medium; performing strain preservation, performing strain activation and preparing bacterium suspension; establishing a standard curve of OD value-viable bacterium content log lgC, and performing calibration on C of inoculated bacterial liquid; establishing a standard curve of lgC-relative fluorescence intensity log lgI; performing sample inoculation, performing sample culture and performing eluting and recovering; determining I of recovered liquid, and reckoning the content C and T of the viable bacteria; calculating bacterium resisting rate R or bacterium resisting activity value A; and performing result evaluation. The method has the characteristic that the mold resisting properties of the electrical household appliances, which are expressed by R or A, can be accurately and quantitatively tested through an ATP fluorophotometer. Control samples and bacterium resisting samples after contact for 0h and culturing for 48h are eluted and recovered according to the specification of the invention, the I of the recovered liquid of the control samples and the I of the recovered liquid of the bacterium resisting samples are determined and are expressed with lgI, and R or A is calculated; and a result evaluation base is provided. According to the ATP biofluorescence lgC-lgI standard curve method for detecting the mold resisting properties of the electrical household appliances, researched and developed by the invention, product quality increase can be supported through a scientific advanced detection technique.

Description

technical field [0001] The invention relates to a method for testing the mildew-proof performance of household appliances, in particular to an ATP bioluminescent IgC that uses an ATP fluorescence photometer to accurately and quantitatively detect the mildew-proof performance of household appliances characterized by antibacterial rate R or antibacterial activity value A B -lgI B The standard curve method belongs to the technical field of antibacterial function detection of household appliances. Background technique [0002] Over the years, the innovation efficiency and market benefits of my country's home appliance industry cannot be underestimated. With the enhancement of people's awareness of health and environmental protection, consumers' antibacterial demand for home appliances is changing with each passing day. At the end of the last century, antibacterial home appliances came into being. The application and popularization of the industry is unstoppable, and the antibact...

AI Technical Summary

Problems solved by technology

In general, the existing anti-mold performance testing methods have the following common problems in technical content and practical application: First, because the mycelium formed during the growth of mold spores cannot be accurately counted, only qualitative judgments can be made, and quantitative tests cannot be performed; The second is that the mold growth cycle is long, and the cultivation time is at least 2 to 4 weeks, resulting in a long test cycle and high time and economic costs; the third is that the experimental process is cumbersome and technically difficult; related operations are greatly affected by human factors, making Large test error, lack of comparability

Driven by the demand, foreign antibacterial material performance testing technology research field has developed ISO 20743:2007-2013 "Textiles-Determination of antibacterial activity of textile products (textiles- Determination of Antibacterial Performance of Antibacterial Finished Textiles) and ISO 13629-1:2012 "Textiles-Determination of antifungal activity oftextile products. Fluorometric assay of content change to characterize anti-bacteria / mold performance, but it is only applicable to textile materials or microporous materials with water absorption and control sample / mold growth value > 0. In terms of technology, it is not applicable to plastics, glass and other household appliance component materials with non-absorbent hard surfaces and the bacterial growth value of the control sample is <0; at the same time, no clear result calculation formula and measurement uncertainty evaluation are provided

In addition, the relevant antibacterial performance characterization parameters of this standard method are relatively single, only involving the antibacterial activity value A, while the antibacterial rate R commonly used in my country

Method used

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