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51 results about "Atp bioluminescence" patented technology

Use of ATP Bioluminescence for Rapid Detection and Enumeration of Contaminants: The Milliflex Rapid Microbiology Detection and Enumeration System. 109 An easy protocol was developed to quickly enumerate spore contamination in artificially inoculated products with RMDS.

Rapid microbiological detection and reagent for environmental water body

A fast detection method of water microbe includes filtering water sample by microhole filtering film with different hole diameter, carrying out ATP luminous detection for used filtering film and for ATP standard solution and new microhole filtering film, calculating linear regression equation of Lg [ATP] = A+ BLg [CPM] based on logarithmic relation of ATP concentration to its luminous pulse counts and calculating out microbe content in water as well as ATP concentration in sample according CPM value of filtering film.
Owner:GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY +1

Sanitary status on-site rapid detection device and detecting method

The invention discloses a sanitary quality locale rapid detection device and a detection method, the device comprises an auto sampling element, a photoelectricity conversion element and a control, operation and storage display circuit element. The method comprises that: the detected parameters are selected, based on an ATP bioluminescence theory, the detected sample and three groups detection reagents of a body cell lysates agent, a bacterial cell lysates agent and a luciferase-luciferase luminescence reagent are added to a sample pool, each reagent is provided with an auto sample adding device connected with the sample pool, the auto sample adding device controls to inject different reagents to the sample pool according to the detection requirement, the sample surface cleanliness condition is gained by measuring the total ATP content, the bacteria quantity in the sample is quantitatively detected by measuring the ATP content in the bacteria cell. Compared with the traditional device, the detection speed of the invention is fast, the detection time is less than 30 minutes, the degree of automation is high, and the maneuverability is good, the invention can be applicable to the locale rapid detection of health quality in the food, cosmetics, medical, environmental, and other fields.
Owner:INST OF ELECTRONICS CHINESE ACAD OF SCI

Multi-sample microbial pollution fast screening method

This invention discloses a method for quick screening multi sample microbial contamination, which utilizes ATP bioluminescence test to complete large sample test, then utilizing biochip technology making identification and classification of harmful microbial to small sample group with exceeded bacteria sum, overcoming the shortage of ATP bioluminescence test in specificity and test quantity.
Owner:XI AN JIAOTONG UNIV

Kit for anti-interference quick detection of microbe quantity by bioluminescence method

The invention relates to a method and a detection kit for rapidly detecting the number of microorganisms using ATP bioluminescence method. The rapid detection kit includes luciferase and its protective agent, D-luciferin, standard ATP, luminescent detection buffer, acellular ATP remover, microbial cell ATP extractant and other reagents. The standard method for using this detection kit to quickly determine the number of microorganisms is to make an ATP standard curve in a selected bioluminescence instrument and a selected system, and obtain a linear regression equation after taking the logarithm. After removing non-target ATP and microbial The sample solution extracted by ATP is detected in the same instrument and system with the same batch of enzymes for ATP bioluminescence detection, and a blank control is made at the same time to obtain the CPM or RLU value of the sample and the blank, and the net relative luminescence value after removing the blank is taken as logarithm Finally, the ATP concentration of the sample is obtained through the established regression equation. According to the ATP content of microorganisms such as bacteria, yeast, mold and unicellular microalgae, it can be converted into the number of cells of bacteria, yeast, mold spores or unicellular microalgae, or only calculated Equivalent to the number of bacteria.
Owner:GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY +1

Method for detecting antibacterial performance of antibacterial plastics by ATP bioluminescence lgC-lgI standard curve method

The invention relates to a method for detecting the antibacterial performance of antibacterial plastics. The method comprises the following detection steps of: carrying out sample preparation and preprocessing; carrying out equipment model selection and reagent and culture medium preparation; carrying out culture preservation and activation, and preparing bacterial suspension; establishing an optical density (OD) value-viable bacterium content logarithm value lgC standard curve, and carrying out inoculation bacteria solution C calibration; establishing a lgC-relative fluorescence intensity logarithm value lgC standard curve; carrying out sample inoculation, culture, elution and recovery; measuring recovery liquid I, and reckoning the viable bacterium contents C and T of the recovery liquid; calculating an antibacterial ratio R or an antibacterial activity value A; and carrying out result evaluation. The method is characterized in that an adenosine triphosphate (ATP) fluorophotometer is applied to accurately and quantitatively test the plastic antibacterial performance represented by R or A. The method stipulates that control samples and antibacterial samples subjected to 0h contact and cultured for 24h are eluted and recovered, the recovery liquid I is measured, R or A is represented and calculated by lgI, and a result evaluation basis is provided. The ATP bioluminescence lgC-lgI standard curve method researched and developed by the invention for detecting the antibacterial performance of the plastics supports product quality improvement by a scientific and advanced detection technology.
Owner:李文杰

Method for detecting anti-fungal performance of nano inorganic material by ATP bioluminescence lgC-lgI standard curve method

PendingCN110272970ADetermination of precisionBreak through the limitations of qualitative analysisFungiMicrobiological testing/measurementStainingModel selection
The invention relates to a method for detecting anti-fungal performance of a nano inorganic antibacterial material. The method for detecting the anti-fungal performance of the nano inorganic antibacterial material comprises the steps that sample preparation and pretreatment are carried out; equipment model selection and reagent and culture medium preparation are carried out; culture preservation, excitation and bacterium suspension preparation are carried out; viable bacterium content C blood cell plate counting of a bacterium suspension after methylene blue staining is carried out; an ATP concentration log value lgC-relative fluorescence intensity log value lgI standard curve is established and an inoculating bacterium solution C is calibrated; sample inoculation and cultivation are carried out; recycled solution I measurement and ATP concentration C and T calculation are carried out; an antibacterial ratio R or an antibacterial activity value A is calculated; and a result is evaluated. The method for detecting the anti-fungal performance of the nano inorganic antibacterial material is characterized in that an ATP fluorophotometer is used for accurately and quantitatively testing the anti-fungal performance of the nano inorganic material represented by R or A. According to the method for detecting the anti-fungal performance of the nano inorganic antibacterial material, it is stipulated that control samples and antibacterial samples after shake cultivation are recycled, and I of the control samples and the antibacterial samples is measured and lgI is used for representing and calculating R or A; and a result evaluation basis is provided. According to an ATP bioluminescence lgC-lgI standard curve method for detecting the anti-fungal performance of the nano inorganic material, an advanced detection technology can be used for supporting product quality improvement.
Owner:李文杰

Method for detecting antibacterial performance of antibacterial paint by ATP bioluminescence lgC-lgI standard curve method

The invention relates to a method for detecting the antibacterial performance of antibacterial paint. The method comprises the following detection steps of: carrying out sample extraction, preparation and preprocessing; carrying out equipment model selection and reagent and culture medium preparation; carrying out culture preservation and activation, and preparing bacterial suspension; establishing an ATP concentration logarithm value lgC-relative fluorescence intensity logarithm value lgI standard curve, and carrying out calibration on inoculation bacteria solution viable bacterium ATP concentration C; carrying out sample inoculation, culture, elution and recovery; measuring recovery liquid I, and reckoning the viable bacterium ATP concentrations C and T of the recovery liquid; calculating an antibacterial ratio R or an antibacterial activity value A; and carrying out result evaluation. The method is characterized in that an ATP fluorophotometer is applied to accurately and quantitatively test the paint antibacterial performance represented by R or A. The method stipulates that control samples and antibacterial samples subjected to 0h contact and cultured for 24h are eluted and recovered, the recovery liquid I is measured, R or A is represented and calculated by lgI, and a result evaluation basis is provided. The ATP bioluminescence lgC-lgI standard curve method researched and developed by the invention for detecting the antibacterial performance of the antibacterial paint supports product quality improvement by a scientific and advanced detection technology.
Owner:INSPECTION & QUARANTINE TECH CENT OF QINHUANGDAO ENTRY EXIT INSPECTION & QUARANTINE BUREAU

Method for detecting antibacterial performance of antibacterial coated glass with ATP (adenosine triphosphate) bioluminescence lgCB-lgIB standard curve method

The invention relates to a method for detecting antibacterial performance of antibacterial coated glass. The method comprises detection steps as follows: sample preparation and pretreatment; selection of equipment type and preparation of reagents and culture media; strain preservation and activation and preparation of a bacterial suspension; establishment of an OD value-viable content logarithm value lgCB standard curve and calibration of an inoculant liquid CB; establishment of an lgCB-relative fluorescence intensity logarithm value lgIB standard curve; inoculation, culture and eluting recovery of samples; IB determination of a recovery liquid and calculation of viable content CB and TB of the recovery liquid; calculation of antibacterial ratio R or antibacterial activity value A; result evaluation. The method is characterized in that an ATP fluorophotometer is applied to accurate and quantitative test of the antibacterial performance of the R or A characterized glass. According to the method, a control sample and an antibacterial sample after 0 h contact and 24 h culture are eluted and recovered, and IB of the recovery liquid is measured and R or A is characterized by lgIB and calculated; result evaluation basis is provided. According to the developed ATP bioluminescence lgCB-lgIB standard curve method for detecting the antibacterial performance of the antibacterial coated glass, product quality improvement is supported with a scientific and advanced detection technology.
Owner:李文杰

Method for evaluating bacterium killing effect of daily chemical products by ATP bioluminescence lgCA-lgIA standard curve method

The invention relates to a method for evaluating bacterium killing effect of daily chemical products. The method comprises detection steps as follows: sample extraction and pretreatment requirement; model selection of equipment and preparation of a reagent and a culture medium; collection and activation of strains and preparation of a bacterial suspension; construction of an ATP concentration log value lgCA-relative fluorescence intensity log value igIA standard curve and viable bacteria ATP concentration CA calibration of an inoculation bacterial solution; neutralizer identification and sterilization test; IA determination of a recovery solution and calculation of viable bacteria ATP concentration CA and TA of the recovery solution; calculation of an antibacterial rate R or antibacterial activity value A; and result evaluation. The method is characterized by applying an ATP fluorophotometer to precisely and quantitatively test the bacterium killing effect of daily chemical products represented by R or A. The method neutralizes and recovers control samples and antibacterial samples after specific time of contamination/killing, determines IA of the recovery solution and represents and calculates R or A by lgIA and provides result evaluation basis. The researched and developed ATP bioluminescence lgCA-lgIA standard curve method for detecting the bacterium killing effect of daily chemical products will support product quality improvement by the scientific and advanced detection technology.
Owner:李文杰

Method for detecting antibacterial performance of polyurethane synthetic leather by ATP bioluminescence lgC-lgI standard curve method

The invention relates to a method for detecting antibacterial performance of polyurethane synthetic leather. The method for detecting the antibacterial performance of the polyurethane synthetic leather comprises the steps that sample preparation and pretreatment are carried out; equipment model selection and reagent and culture medium preparation are carried out; culture preservation, excitation and bacterium suspension preparation are carried out; an OD value-viable bacterium content log value lgC standard curve is established and an inoculating bacterium solution C is calibrated; an lgC relative fluorescence intensity log value lgI standard curve is established; sample inoculating, cultivating, eluting and cultivating are carried out; recycled solution I measurement and variable bacterium content C and T calculation are carried out; an antibacterial ratio R or an antibacterial activity value A is calculated; and a result is evaluated. The method for detecting the antibacterial performance of the polyurethane synthetic leather is characterized in that an ATP fluorophotometer is used for accurately and quantitatively testing the antibacterial performance of the synthetic leather represented by R or A. According to the method for detecting the antibacterial performance of the polyurethane synthetic leather, it is stipulated that control samples and antibacterial samples are recovered by elution after contact for 0h and cultivation for 24h and cycled, and a recycled solution I is measured and lgI is used for representing and calculating R or A; and a result evaluation basis is provided. According to an ATP bioluminescence lgC-lgI standard curve method for detecting antibacterial performance of the polyurethane synthetic leather, an advanced detection technology can be used for supporting product quality improvement.
Owner:李文杰

Bioaerosol early warning device based on ATP bioluminescence technology

The invention relates to the technical field of air microorganism monitoring, and discloses a bioaerosol early warning device based on an ATP bioluminescence technology. The device comprises a reagent conveying module used for containing a reaction reagent and conveying the reaction reagent to a fluorescence reaction module; a sampling module used for collecting and conveying microorganisms in ambient air to the fluorescence reaction module; the fluorescence reaction module used for mixing the reaction reagent with the microorganisms and generating fluorescence; a monitoring and processing module used for detecting the fluorescence change, converting the fluorescence change into an electric signal, processing the electric signal to obtain the concentration of microorganisms, and sending an early warning signal when the concentration exceeds a preset value; and a waste liquid collection module used for collecting waste liquid after reaction of the fluorescence reaction module. The change of the concentration of microorganisms in air is obtained based on the ATP bioluminescence technology, an early warning signal is sent out when the concentration exceeds a preset value, the overall structure is simple, the function is reliable, the implementation is easy, and the working efficiency of the whole device can be improved.
Owner:北京鼎蓝科技有限公司
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