Method for detecting antibacterial performance of antibacterial paint by ATP bioluminescence lgC-lgI standard curve method

Abstract

The invention relates to a method for detecting the antibacterial performance of antibacterial paint. The method comprises the following detection steps of: carrying out sample extraction, preparation and preprocessing; carrying out equipment model selection and reagent and culture medium preparation; carrying out culture preservation and activation, and preparing bacterial suspension; establishing an ATP concentration logarithm value lgC-relative fluorescence intensity logarithm value lgI standard curve, and carrying out calibration on inoculation bacteria solution viable bacterium ATP concentration C; carrying out sample inoculation, culture, elution and recovery; measuring recovery liquid I, and reckoning the viable bacterium ATP concentrations C and T of the recovery liquid; calculating an antibacterial ratio R or an antibacterial activity value A; and carrying out result evaluation. The method is characterized in that an ATP fluorophotometer is applied to accurately and quantitatively test the paint antibacterial performance represented by R or A. The method stipulates that control samples and antibacterial samples subjected to 0h contact and cultured for 24h are eluted and recovered, the recovery liquid I is measured, R or A is represented and calculated by lgI, and a result evaluation basis is provided. The ATP bioluminescence lgC-lgI standard curve method researched and developed by the invention for detecting the antibacterial performance of the antibacterial paint supports product quality improvement by a scientific and advanced detection technology.

Description

technical field [0001] The present invention relates to the antibacterial property testing method of antibacterial coating, specifically a kind of ATP bioluminescent IgC that uses ATP fluorescence photometer to carry out accurate quantitative detection antibacterial performance of antibacterial coating represented by antibacterial rate R or antibacterial activity value A A -lgI A The standard curve method belongs to the technical field of paint antibacterial function detection. Background technique [0002] With the improvement of people's living standards and the enhancement of health awareness, the indoor environment and the quality of home decoration have attracted much attention, so antibacterial coatings have emerged at the historic moment and the supply is in short supply; according to statistics, the global production of antibacterial coatings in 2017 has exceeded 400,000 tons; the relevant organization Zion Research's latest research report shows that the internatio...

AI Technical Summary

Problems solved by technology

So far, although my country has promulgated and implemented GB / T 21866-2008 "Antibacterial Coating (Paint Film) Antibacterial Determination and Antibacterial Effect", the existing detection methods have the following common problems in technical content and practical application: First, all There are too few strains involved in the experiment, and it is difficult to meet the new product development and quality control needs of the enterprise to keep pace with the times; second, the experimental process is cumbersome, and the relevant operations are affected by the professional experience of the experimenter, and the test error is large, lacking comparability; third, the test cycle is within Between 48 hours and 72 hours, the time and economic cost are high

At present, the research on performance testing technology of antibacterial materials in foreign countries is developing towards quantification, rapidity and simplification, focusing on the accuracy and comparability of test results; the principle of ATP fluorescence analysis has been used to formulate ISO 20743:2007-2013 Textiles-Determination of antibacterial activity of textile products (textiles - Determination of antibacterial properties of antibacterial finishing textiles)" and ISO13629-1:2012 "Textiles-Determination of antifungal activity of textile products. The change of ATP content after inoculation is a fluorometric method to characterize the anti-microbial / mold performance, but it is only applicable to textile materials or microporous materials with water absorption and the growth value of the control sample / mycotic bacteria>0. Conditions and other key technical aspects are not applicable to coating floor materials such as building materials and metals with non-absorbent hard surfaces and control sample bacterial growth value <0; at the same time, no clear result calculation formula and measurement uncertainty evaluation are provided

In addition, the relevant antibacterial performance characterization parameters of this standard method are relatively single, only involving the antibacterial activity value A, while the antibacterial rate R commonly used in my country

Images