Method for detecting antibacterial performance of polyurethane synthetic leather by ATP bioluminescence lgC-lgI standard curve method

Abstract

The invention relates to a method for detecting antibacterial performance of polyurethane synthetic leather. The method for detecting the antibacterial performance of the polyurethane synthetic leather comprises the steps that sample preparation and pretreatment are carried out; equipment model selection and reagent and culture medium preparation are carried out; culture preservation, excitation and bacterium suspension preparation are carried out; an OD value-viable bacterium content log value lgC standard curve is established and an inoculating bacterium solution C is calibrated; an lgC relative fluorescence intensity log value lgI standard curve is established; sample inoculating, cultivating, eluting and cultivating are carried out; recycled solution I measurement and variable bacterium content C and T calculation are carried out; an antibacterial ratio R or an antibacterial activity value A is calculated; and a result is evaluated. The method for detecting the antibacterial performance of the polyurethane synthetic leather is characterized in that an ATP fluorophotometer is used for accurately and quantitatively testing the antibacterial performance of the synthetic leather represented by R or A. According to the method for detecting the antibacterial performance of the polyurethane synthetic leather, it is stipulated that control samples and antibacterial samples are recovered by elution after contact for 0h and cultivation for 24h and cycled, and a recycled solution I is measured and lgI is used for representing and calculating R or A; and a result evaluation basis is provided. According to an ATP bioluminescence lgC-lgI standard curve method for detecting antibacterial performance of the polyurethane synthetic leather, an advanced detection technology can be used for supporting product quality improvement.

Description

technical field [0001] The invention relates to an antibacterial performance testing method of antibacterial polyurethane synthetic leather, specifically an ATP bioluminescence method for accurately and quantitatively detecting the antibacterial performance of antibacterial polyurethane synthetic leather characterized by antibacterial rate R or antibacterial activity value A by using an ATP fluorescence photometer lgC B -lgI B The standard curve method belongs to the technical field of leather antibacterial function detection. Background technique [0002] Leather is a necessity of human life. Due to the introduction of a large amount of fatliquor in the process of tanning and the use of raw hides rich in protein, fat and other nutrients, it is extremely susceptible to microbial infection such as bacteria and fungi. With the improvement of people's living standards and enhanced health awareness, the requirements for antibacterial performance of leather products continue to...

AI Technical Summary

Problems solved by technology

Up to now, my country has formulated and implemented QB / T 4341-2012 "Antibacterial Polyurethane Synthetic Leather Antibacterial Performance Test Method and Antibacterial Effect", but the existing detection methods have the following common problems in technical content and practical application: First, the experimental bacteria involved If there are too few species, it is difficult to meet the new product development and quality control needs of the enterprise to keep pace with the times; second, the experimental process is cumbersome, and the relevant operations are affected by the professional experience of the experimenter, and the test error is large, lacking comparability; third, the test cycle is 48 hours~ Between 72 hours, time and economic costs are high

At present, the research on performance testing technology of antibacterial materials in foreign countries is developing towards quantification, rapidity and simplification, focusing on the accuracy and comparability of test results; the principle of ATP fluorescence analysis has been used to formulate ISO20743:2007-2013 "Textiles-Determination of antibacterial activity of textile products (Textiles - Determination of antibacterial performance of antibacterial finishing textiles)" and ISO13629-1:2012 "Textiles-Determination of antifungal activity of textile products. Part1 Luminescence (Textiles - Determination of antifungal performance of antibacterial finishing textiles)", which stipulates that inoculation of samples Fluorescence measurement method to characterize anti-microbial / mold performance by ATP content change, but it is only applicable to textile materials or microporous materials with water absorption and control sample bacterial / mold growth value > 0, and it is not suitable for key technologies such as effective conditions of the test. Applicable to synthetic leather materials and products with poor water absorption and bacterial growth value of the control sample <0; at the same time, no clear result calculation formula and measurement uncertainty evaluation are provided

In addition, the relevant antibacterial performance characterization parameters of this standard method are relatively single, only involving the antibacterial activity value A, while the antibacterial rate R commonly used in my country

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine

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