Method for detecting anti-fungal performance of nano inorganic material by ATP bioluminescence lgC-lgI standard curve method

A nano-inorganic and detection method technology, which is applied in the field of ATP biofluorescence lgCA-lgIA standard curve method and anti-fungal performance test of nano-inorganic antibacterial materials, can solve the problem of not providing measurement uncertainty evaluation and not applicable to nano-scale powder type Problems such as inorganic antibacterial materials and single characterization parameters of antibacterial performance have achieved the effect of breaking through the limitations of qualitative analysis, improving scientificity and versatility, and shortening the test cycle

Pending Publication Date: 2019-09-24
李文杰
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Problems solved by technology

In view of the current development trend of quantification, rapidity and simplification in the research field of foreign antibacterial material performance testing technology, the principle of ATP fluorescence analysis has been used to formulate ISO 20743:2007-2013 "Textiles-Determination of antibacterial activity of textile products (textiles-antibacterial finishing textiles) Determination of antibacterial performance of textiles)" and ISO 13629-1:2012 "Textiles-Determination of antifungal activity of textile products.Part1 Luminescence (textiles - Determination of antifungal performance of antibacterial finishing textiles)", which stipulates that the change of ATP content after sample inoculation is used to characterize Fluorescence measurement m

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  • Method for detecting anti-fungal performance of nano inorganic material by ATP bioluminescence lgC-lgI standard curve method
  • Method for detecting anti-fungal performance of nano inorganic material by ATP bioluminescence lgC-lgI standard curve method
  • Method for detecting anti-fungal performance of nano inorganic material by ATP bioluminescence lgC-lgI standard curve method

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Embodiment Construction

[0142] The following describes the present invention in detail with reference to preferred embodiments, so that the advantages and features of the present invention can be more easily understood by those skilled in the art, so as to make a clearer and clearer definition of the protection scope of the present invention.

[0143] In this embodiment, the antifungal performance test of a nano inorganic antibacterial powder material sample prepared by adding a nano silver antibacterial agent is taken as an example for description.

[0144] The specific detection method is carried out according to the following steps:

[0145] (1) Sample preparation and pretreatment

[0146] 1.1 Control sample: Silica powder without antibacterial treatment, particle size not greater than 100nm, purity 98%~99%; pour 0.5g±0.05g sample powder and 44mL test culture broth containing 0.1% Tween-80 Put it into a 100mL conical flask, seal it and autoclave at 121°C for 30min. Each strain test uses 6 groups of sampl...

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Abstract

The invention relates to a method for detecting anti-fungal performance of a nano inorganic antibacterial material. The method for detecting the anti-fungal performance of the nano inorganic antibacterial material comprises the steps that sample preparation and pretreatment are carried out; equipment model selection and reagent and culture medium preparation are carried out; culture preservation, excitation and bacterium suspension preparation are carried out; viable bacterium content C blood cell plate counting of a bacterium suspension after methylene blue staining is carried out; an ATP concentration log value lgC-relative fluorescence intensity log value lgI standard curve is established and an inoculating bacterium solution C is calibrated; sample inoculation and cultivation are carried out; recycled solution I measurement and ATP concentration C and T calculation are carried out; an antibacterial ratio R or an antibacterial activity value A is calculated; and a result is evaluated. The method for detecting the anti-fungal performance of the nano inorganic antibacterial material is characterized in that an ATP fluorophotometer is used for accurately and quantitatively testing the anti-fungal performance of the nano inorganic material represented by R or A. According to the method for detecting the anti-fungal performance of the nano inorganic antibacterial material, it is stipulated that control samples and antibacterial samples after shake cultivation are recycled, and I of the control samples and the antibacterial samples is measured and lgI is used for representing and calculating R or A; and a result evaluation basis is provided. According to an ATP bioluminescence lgC-lgI standard curve method for detecting the anti-fungal performance of the nano inorganic material, an advanced detection technology can be used for supporting product quality improvement.

Description

Technical field [0001] The invention relates to a method for testing the antifungal performance of nano inorganic antibacterial materials, in particular to an ATP biofluorescence that uses an ATP fluorescence photometer to accurately and quantitatively detect the antifungal performance of nano inorganic antibacterial materials characterized by antibacterial rate R or antibacterial activity value A lgC A -lgI A The standard curve method belongs to the technical field of antibacterial function detection of nano-inorganic materials. Background technique [0002] In recent years, with the rapid progress of social science and technology and the increasing awareness of people’s health and environmental protection; nano-inorganic antibacterial materials are widely used in many fields such as building materials, home appliances, textiles, food bags, daily chemicals, and their domestic market scale has already exceeded 100 billion. And it is increasing year by year, but the current lag of...

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Application Information

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IPC IPC(8): C12Q1/18C12Q1/06C12N1/14C12N1/16C12R1/725C12R1/685C12R1/82C12R1/645
CPCC12Q1/18C12Q1/06C12N1/14C12N1/16C12Q2304/60G01N2333/385G01N2333/40G01N2333/38G01N2333/37
Inventor 李文杰高月红孙计瓒郝凌云靳慧达
Owner 李文杰
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