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Multi-sample microbial pollution fast screening method

A microbial contamination, multi-sample technology, applied in the fields of medicine, public safety, and biology, to achieve quantitative detection, easy on-site operation, and simple process.

Inactive Publication Date: 2006-03-08
XI AN JIAOTONG UNIV
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  • Abstract
  • Description
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Problems solved by technology

[0005] Aiming at the problems existing in the existing detection of microbial contamination, the object of the present invention is to provide a method for rapid screening of multi-sample microbial contamination, which is completely different from the traditional method of performing the same detection on each sample without distinction, greatly improving the Improve the detection efficiency and meet the actual requirements for rapid on-site detection of large sample microbial contamination

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  • Multi-sample microbial pollution fast screening method

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Embodiment Construction

[0033] refer to figure 1 , the multi-sample microbial pollution rapid screening system prepared according to the method of the present invention realizes the on-site rapid screening of large sample microbial contamination. What is used is a multi-link chip, which is different from ordinary multi-link biochips. This multi-link chip will The ATP bioluminescent linker is also immobilized on the chip microarray, so that the same chip can be used for the screening and specific detection of large samples of harmful microorganisms. The samples bound to the chip can be detected by the fluorescence detection device, and various projects can be completed at one time. For the detection of ATP, Escherichia coli, Shigella, Candida albicans, etc., the chip can also be replaced according to the actual situation to improve the detection efficiency. In addition, the advantages of the system are especially significant when the sample size is large. Specifically, for the collected samples, firs...

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Abstract

This invention discloses a method for quick screening multi sample microbial contamination, which utilizes ATP bioluminescence test to complete large sample test, then utilizing biochip technology making identification and classification of harmful microbial to small sample group with exceeded bacteria sum, overcoming the shortage of ATP bioluminescence test in specificity and test quantity.

Description

technical field [0001] The invention relates to the fields of biology, medicine and public safety, in particular to a method for rapidly screening multi-sample microbial contamination by using biological ATP (adenosine triphosphate) fluorescence energy method combined with modern biochip technology. Background technique [0002] As cosmetics, food, pharmaceuticals and other public health services have increasingly stringent requirements on the control and detection of microorganisms, it is urgent to propose a new rapid detection method and corresponding automated instruments to monitor the cleanliness of the production environment in real time. However, the microbial detection method stipulated in the national standard is still the agar plate culture method. The main defect of this method is that it takes a long time (24 hours for bacteria, 96 hours for molds, and longer time for some microorganisms such as mycobacteria, etc.). [0003] In addition, t...

Claims

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Application Information

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IPC IPC(8): G01N21/76C12Q1/02
Inventor 彭年才张镇西李炜冀晓燕
Owner XI AN JIAOTONG UNIV
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