Method for detecting antibacterial performance of antibacterial coated glass with ATP (adenosine triphosphate) bioluminescence lgCB-lgIB standard curve method

Abstract

The invention relates to a method for detecting antibacterial performance of antibacterial coated glass. The method comprises detection steps as follows: sample preparation and pretreatment; selection of equipment type and preparation of reagents and culture media; strain preservation and activation and preparation of a bacterial suspension; establishment of an OD value-viable content logarithm value lgCB standard curve and calibration of an inoculant liquid CB; establishment of an lgCB-relative fluorescence intensity logarithm value lgIB standard curve; inoculation, culture and eluting recovery of samples; IB determination of a recovery liquid and calculation of viable content CB and TB of the recovery liquid; calculation of antibacterial ratio R or antibacterial activity value A; result evaluation. The method is characterized in that an ATP fluorophotometer is applied to accurate and quantitative test of the antibacterial performance of the R or A characterized glass. According to the method, a control sample and an antibacterial sample after 0 h contact and 24 h culture are eluted and recovered, and IB of the recovery liquid is measured and R or A is characterized by lgIB and calculated; result evaluation basis is provided. According to the developed ATP bioluminescence lgCB-lgIB standard curve method for detecting the antibacterial performance of the antibacterial coated glass, product quality improvement is supported with a scientific and advanced detection technology.

Description

technical field [0001] The invention relates to a method for testing the antibacterial performance of coated antibacterial glass, in particular to an ATP bioluminescent IgC that uses an ATP fluorescence photometer to accurately and quantitatively detect the antibacterial performance of coated antibacterial glass characterized by antibacterial rate R or antibacterial activity value A B -lgI B The standard curve method belongs to the technical field of glass antibacterial function detection. Background technique [0002] As a new type of ecological functional material, antibacterial glass adopts nano "dispersion" and "modification" technology to combine antibacterial agent with glass in a chemical bonding way, which belongs to the highest level of coated glass; it is used in medical care, food preservation, transportation and 3C electronic touch products and other fields are widely used. According to the global market observation report, the antibacterial glass market size in...

AI Technical Summary

Problems solved by technology

In general, the existing antibacterial performance detection methods have the following common problems in technical content and practical application: First, the experimental strains involved are too small to meet the needs of enterprises for new product development and quality control that keep pace with the times; The experimental process is cumbersome, and the relevant operations are affected by the professional experience of the experimenter, the test error is large, and there is no comparability; the third is that the test cycle is between 48 hours and 72 hours, and the time and economic costs are high

At present, the research on the performance testing technology of antibacterial materials in foreign countries is developing toward the trend of quantification, rapidity and simplification, focusing on the accuracy and comparability of test results; the principle of ATP fluorescence analysis has been used to formulate ISO20743:2007-2013 "Textiles-Determination of antibacterial activity oftextile products ( Textiles - Determination of antibacterial performance of antibacterial finishing textiles)" and ISO13629-1:2012 "Textiles-Determination of antifungal activity of textile products. Fluorometric assay of content change to characterize anti-bacteria / mold performance, but it is only applicable to textile materials or microporous materials with water absorption and control sample / mold growth value > 0. In terms of technology, it is not applicable to inorganic non-metallic materials such as glass and ceramics with non-absorbent hard surfaces and control bacteria growth value <0; at the same time, no clear result calculation formula and measurement uncertainty evaluation are provided

In addition, the relevant antibacterial performance characterization parameters of this standard method are relatively single, only involving the antibacterial activity value A, while the antibacterial rate R commonly used in my country

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