A method and apparatus is described by which means molecules in suspension may be characterized in terms of the size and
mass distributions present. As a sample solution is separated by centrifugal means, it is illuminated at a particular radial distance from the axis of rotation by a fine, preferably monochromatic,
light beam. Despite the
high resolution of such devices, a key problem associated with most separators based upon use of centrifugal forces is the difficulty in deriving the absolute size and / or
molar.
mass of the separating molecules. By integrating means to detect light scattered, over a range of scattering angles, from samples undergoing
centrifugal separation, molecular sizes in the sub-
micrometer range may be derived, even in the presence of
diffusion. Adding a second
light beam at a displaced rotational angle, preferably of an
ultraviolet wavelength, that intersects the sample at the same radial region as the first beam permits determination of the molecular concentration at that region. Combining the
light scattering data with the associated concentration permits the determination of the associated
molar mass. In a preferred embodiment, the
light beam and detectors may be controlled to scan synchronously the sample radially during separation.