Method for detecting anti-mildew property of antibacterial plastics with ATP (adenosine triphosphate) bioluminescence lgCA-lgIA standard curve method

Abstract

The invention relates to a method for detecting anti-mildew property of antibacterial plastics. The method comprises detection steps as follows: sample preparation and pretreatment; selection of equipment type and preparation of reagents and culture media; strain preservation and activation and preparation of a spore solution; establishment of an OD value-viable content logarithm value lgCB standard curve; establishment of an ATP (adenosine triphosphate) concentration logarithm value lgCA-relative fluorescence intensity logarithm value lgIA standard curve and calibration of viable bacteria CA of an inoculant liquid; inoculation, culture and eluting recovery of samples; IA determination of a recovery liquid and calculation of viable bacteria ATP concentration CA and TA of the recovery liquid; calculation of antibacterial ratio R or antibacterial activity value A; result evaluation. The method is characterized in that an ATP fluorophotometer is applied to accurate and quantitative test of the anti-mildew property of R or A characterized antibacterial plastics. According to the method, a control sample and an antibacterial sample after 0 h contact and 24 h culture are eluted and recovered, and IA of the recovery liquid is measured and R or A is characterized by lgIA and calculated; result evaluation basis is provided. According to the developed ATP bioluminescence lgCA-lgIA standard curve method for detecting the anti-mildew property of the plastics, product quality improvement is supported with a scientific and advanced detection technology.

Description

technical field [0001] The invention relates to a method for testing the anti-mold performance of antibacterial plastics, in particular to an ATP bioluminescent IgC that uses an ATP fluorescence photometer to accurately and quantitatively detect the anti-mold performance of antibacterial plastics characterized by antibacterial rate R or antibacterial activity value A A -lgI A The standard curve method belongs to the technical field of plastic antibacterial function detection. Background technique [0002] Plastic products are widely used in the fields of industry and people's livelihood. Because the additives used in the processing process provide the necessary nutrient source for the growth of bacteria, molds and other microorganisms, the sterility of plastics and their surfaces is very important for reducing the spread of diseases and improving people's health. level is very important. Since the 1990s, developed countries represented by Japan and the United States have b...

AI Technical Summary

Problems solved by technology

In general, there are the following common problems in the technical content and practical application of the anti-mold performance detection method: First, because the mycelium formed during the growth of mold spores cannot be accurately counted, only qualitative judgments can be made, and quantitative tests cannot be performed; Due to the long growth cycle of mold, the cultivation time is at least 2 to 4 weeks, resulting in a long test cycle, high time and economic costs; third, the experimental process is cumbersome and technically difficult; related operations are greatly affected by human factors, resulting in test errors large, incomparable

Driven by the demand, foreign antibacterial material performance testing technology research field aimed at the current development trend of quantification, rapidity and simplification, has drawn on the principle of ATP fluorescence analysis to formulate ISO20743:2007-2013 "Textiles-Determination of antibacterial activity of textile products (textiles- Determination of Antibacterial Performance of Antibacterial Finished Textiles)" and ISO13629-1:2012 "Textiles-Determination of antifungalactivity of textile products. Fluorometric method to characterize the anti-microbial / mold performance, but it is only applicable to textile or microporous materials with water absorption and control sample / mycotic growth value > 0, and it is not suitable for key technologies such as the recovery method of viable bacteria and the effective conditions of the test. Applicable to plastics, glass and other materials with non-absorbent hard surface and mold growth value of the control sample < 0, and does not provide measurement uncertainty evaluation

In addition, the relevant antibacterial performance characterization parameters of this standard method are relatively single, only involving the antibacterial activity value A, while the antibacterial rate R commonly used in my country

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